【摘要】： 目的 妊娠期合理的營養(yǎng)攝入能增加子代健康的機會,孕期營養(yǎng)對子代的發(fā)育和遠期影響越來越成為人們關注的熱門話題。本研究擬通過在圍孕期給母鼠服用益生菌制劑金雙歧,來進一步觀察圍孕期應用益生菌是否對子代大鼠腸道免疫功能成熟有促進作用。 方法 健康雄性大鼠6只,健康雌性大鼠30只隨機分組(按雌:雄=5:1)合籠交配。分別于交配前7天(5只)、妊娠早期(第1天始,5只),妊娠中期(第8天始,5只),妊娠晚期(第15天,5只),分娩母乳期(5只)應用益生菌制劑金雙歧(雙歧桿菌、乳桿菌、嗜熱鏈球菌三聯活菌片)連續(xù)灌胃給藥;對照組(不用益生菌,普通喂養(yǎng)5只)。在分娩后第1,2,3,7,14天,采取逼迫法取子代大鼠新鮮糞便,(每只母鼠隨機抽取5只子鼠)接種到改良MRS培養(yǎng)基上,進行厭氧培養(yǎng),觀察結果,記錄菌落數。同時在21天采用ELISA方法測定子代大鼠(每組隨機抽取14只子鼠)血清中IL-4和IFN—r水平,與對照組進行比較。 結果 1.在分娩后48小時內試驗組和對照組子鼠腸道菌群中均未見雙歧桿菌定植,但72小時內均發(fā)現有雙歧桿菌定植。 2.懷孕前或懷孕早期服用益生菌的母鼠所分娩的子鼠血清中IL-4和IFN—r濃度高于對照組子鼠(P＜0.05),僅母乳期應用益生菌幾乎不影響子代IL—4和IFN—r水平(P＞0.05)。 結論 1.孕前、妊娠期和母乳期應用益生菌其子代腸道菌群的提前定植作用不明顯。 2.圍孕期應用益生菌可升高子代血清IL-4和IFN—r含量,隨用藥時間的延長總體上血清中IL-4和IFN—r濃度有升高趨勢,但幅度不同,用藥時間短的和未用藥的血清中IL-4和IFN—r濃度差別不是很大。 3.孕婦在孕前和妊娠期服用益生菌制劑對新生兒生后腸道免疫功能成熟有一定的促進作用。
[Abstract]:Objective reasonable nutrition intake during pregnancy can increase the chances of offspring health, and the influence of nutrition during pregnancy on the development and long-term development of offspring has become a hot topic. The aim of this study was to observe whether probiotics could promote the maturation of intestinal immune function in pregnant rats by taking the bifidus of probiotics preparation during the period of gestation in order to further observe the effect of probiotics on the maturation of intestinal immune function in the offspring rats during peri-pregnancy. Methods six healthy male rats and 30 healthy female rats were randomly divided into two groups (male = 5:1). At 7 days before mating (5), early pregnancy (1 day, 5), middle pregnancy (8 days, 5), third trimester (15 days, 5), The probiotics preparation gold bifidobacterium (Bifidobacterium, Lactobacillus, Streptococcus thermophilus triple viable bacterial tablets) was administered continuously during the milk delivery period (n = 5). Control group (not probiotics, ordinary feeding 5). On the 1st, 2nd, 3rd, 7th, 14th day after delivery, the fresh feces of the offspring rats were taken by forced method and inoculated into the modified MRS medium at random. The results were observed and the number of colonies was recorded. At the same time, the levels of IL-4 and IFN-r in the serum of 14 offspring rats were measured by ELISA method on the 21st day and compared with those of the control group. Outcome 1. Bifidobacterium colonization was not found in the intestinal flora of the test group and control group within 48 hours after delivery, but bifidobacterium colonization was found within 72 hours. 2. The serum levels of IL-4 and IFN-r in prepregnant and early pregnant mother mice were higher than those in control group (P < 0.05). Probiotics only had no effect on the levels of IL-4 and IFN-r in their offspring (P > 0.05). Conclusion 1. Before pregnancy, preimplantation of probiotics during pregnancy and breast-feeding had no significant effect on the early colonization of the intestinal flora of their offspring. 2. Probiotics could increase the levels of serum IL-4 and IFN-r in offspring during perinatal period. The concentrations of IL-4 and IFN-r in serum tended to increase with the prolongation of the treatment time, but the range was different. The concentrations of IL-4 and IFN-r in the serum of patients with and without medication were not significantly different. 3. The use of probiotics in pregnant women before and during pregnancy can promote the maturation of intestinal immune function in newborn infants.
【學位授予單位】：中國醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2008
【分類號】：R371;R715

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