【摘要】：目的探討15Hz、1mT電磁場對骨髓間充質(zhì)干細(xì)胞成軟骨分化指標(biāo)聚集蛋白聚糖(AgcI),II型膠原和Sox9等的影響及初步機(jī)制。 方法體外培養(yǎng)SD大鼠骨髓間充質(zhì)干細(xì)胞,取第3代細(xì)胞,用15Hz、1mT電磁場(EMFs)刺激,8h/d。采用半定量逆轉(zhuǎn)錄聚合酶鏈反應(yīng)(RT-PCR)法檢測甲狀旁腺激素激素受體相關(guān)肽(PTHrp)、Agc I、II型膠原和Sox9等mRNA的表達(dá),用Western Blot檢測II型膠原蛋白的表達(dá)。加入解蛋白激酶A(PKA)抑制劑H-89和蛋白激酶C(PKC)抑制劑Go-6976,并再次用RT-PCR法和Western Blot觀察EMFs對Agc I、II型膠原和Sox9等mRNA的表達(dá)的影響。 結(jié)果EMFs促進(jìn)骨髓間充質(zhì)干細(xì)胞PTHrp、Agc I、II型膠原和Sox9等mRNA的表達(dá),促進(jìn)II型膠原蛋白的表達(dá);在加入H-89(10μm)和Go-6976(12μm)后EMFs的這種促進(jìn)Agc I和II型膠原的效應(yīng)就明顯減弱,但Sox9的表達(dá)不受影響。 結(jié)論EMFs促進(jìn)骨髓間充質(zhì)干細(xì)胞成軟骨分化指標(biāo)AgcI、II型膠原和Sox9等mRNA的表達(dá),這種促進(jìn)作用同PKA和PKC通路有聯(lián)系。
[Abstract]:Aim to investigate the effects of 15Hz and 1mT electromagnetic fields on the aggregation of proteoglycan (AgcI), type II collagen and Sox9 in chondrogenic differentiation of bone marrow mesenchymal stem cells (MSCs). Methods SD rat bone marrow mesenchymal stem cells were cultured in vitro. The third generation cells were stimulated with 15Hz, 1mT electromagnetic field (EMFs) for 8 h. Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression of type II collagen and Sox9, and Western Blot was used to detect the expression of type II collagen in parathyroid hormone receptor-related peptide (PTHrp), Agc I,. The effects of EMFs on the expression of Agc I, type II collagen, Sox9 and other mRNA were observed by RT- PCR and Western Blot. The expression of Agc I, collagen II and Sox9 were observed by RT- PCR and Western Blot. Results EMFs promoted the expression of mRNA such as PTHrp,Agc I, type II collagen and Sox9, and promoted the expression of type II collagen in bone marrow mesenchymal stem cells. The effect of EMFs on Agc type I and type II collagen was significantly decreased after addition of Go-6976 (12 渭 m) and 10 渭 m (10 渭 m), but the expression of Sox9 was not affected by the addition of Hx89 (10 渭 m) and Agc (12 渭 m). Conclusion EMFs promotes the expression of mRNA such as AgcI, type II collagen and Sox9, which is related to PKA and PKC pathway.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R329

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 朱智奇;江建明;冷燕奎;蔡東嶺;;SOX9、結(jié)締組織生長因子促進(jìn)腱骨結(jié)合處愈合的生物力學(xué)研究[J];細(xì)胞與分子免疫學(xué)雜志;2011年07期

2 ;[J];;年期

3 ;[J];;年期

4 ;[J];;年期

5 ;[J];;年期

6 ;[J];;年期

7 ;[J];;年期

8 ;[J];;年期

9 ;[J];;年期

10 ;[J];;年期

相關(guān)會議論文 前10條

1 王朝金;袁振;穆立軍;汪先虎;;北鋼1780熱連軋AGC的實際應(yīng)用[A];2010年全國軋鋼生產(chǎn)技術(shù)會議文集[C];2010年

2 賈永強(qiáng);;五機(jī)架冷連軋AGC厚度控制系統(tǒng)[A];2010年全國軋鋼生產(chǎn)技術(shù)會議文集[C];2010年

3 文成宇;張_g;李e，

本文編號：2450112