年青人骨髓間充質(zhì)干細胞來源生物活性因子對年老人骨髓間充質(zhì)干細胞功能的影響
發(fā)布時間：2019-01-19 13:41
【摘要】：目的探討年青人骨髓間充質(zhì)干細胞(h MSCs)來源生物活性因子對年老人h MSCs功能的影響。方法低氧無血清培養(yǎng)條件下獲得年青及年老h MSCs條件培養(yǎng)基(CM)。將年老h MSCs置于年青h MSCs來源CM中,或相反將年青h MSCs置于年老h MSCs來源CM中。通過Transwell實驗、油紅O染色、茜素紅染色、β半乳糖苷酶染色、qRT-PCR分別檢測不同來源CM中生物活性因子對h MSCs遷移能力、向脂肪細胞及成骨細胞分化能力、衰老、microRNA表達的影響。結(jié)果年青h MSCs來源CM生物活性因子可促進年老h MSCs功能再生,增強遷移能力和向脂肪細胞及成骨細胞分化能力,減少衰老。相反,將年青h MSCs置于年老h MSCs來源CM中,年青h MSCs功能受損,遷移能力下降,向脂肪細胞及成骨細胞分化能力下降,衰老增加。qRT-PCR檢測發(fā)現(xiàn),年青h MSCs來源CM生物活性因子可促進h MSCs中miR-10a表達,而年老h MSCs來源CM生物活性因子可抑制其表達。結(jié)論年青h MSCs來源生物活性因子可促進miR-10a表達,促進h MSCs遷移、分化,減少細胞衰老,促進年老h MSCs功能再生。
[Abstract]:Objective to investigate the effects of bioactive factors derived from (h MSCs) from bone marrow mesenchymal stem cells (BMSCs) on the function of MSCs in the elderly. Methods (CM). Was obtained from young and old MSCs conditioned medium under hypoxic serum-free culture condition. The old h MSCs was placed in the young h MSCs source CM, or the younger h MSCs was placed in the old h MSCs source CM. Transwell assay, oil red O staining, alizarin red staining, 尾 -galactosidase staining and qRT-PCR were used to detect the ability of bioactive factors in CM to migrate to h MSCs, differentiate into adipocytes and osteoblasts, and senescence. The effect of microRNA expression. Results the bioactive factor of CM derived from young h MSCs could promote the functional regeneration of MSCs, enhance the ability of migration and differentiation into adipocytes and osteoblasts, and reduce senescence. On the contrary, the young h MSCs was placed in the aged h MSCs source CM, the function of the young h MSCs was impaired, the migration ability was decreased, the differentiation ability to adipocytes and osteoblasts was decreased, and the senescence was increased. The bioactive factor of CM derived from young h MSCs could promote the expression of miR-10a in h MSCs, but the bioactive factor derived from CM from old h MSCs could inhibit its expression. Conclusion the bioactive factor derived from young h MSCs can promote the expression of miR-10a, promote the migration and differentiation of h MSCs, reduce cell senescence and promote the functional regeneration of h MSCs.
【作者單位】： 廣州醫(yī)科大學附屬第二醫(yī)院廣州心血管疾病研究所;
【基金】：國家自然科學基金資助項目(編號:81401156) 廣州市醫(yī)藥衛(wèi)生科技項目(編號:20141A011085)
【分類號】：R329
[Abstract]:Objective to investigate the effects of bioactive factors derived from (h MSCs) from bone marrow mesenchymal stem cells (BMSCs) on the function of MSCs in the elderly. Methods (CM). Was obtained from young and old MSCs conditioned medium under hypoxic serum-free culture condition. The old h MSCs was placed in the young h MSCs source CM, or the younger h MSCs was placed in the old h MSCs source CM. Transwell assay, oil red O staining, alizarin red staining, 尾 -galactosidase staining and qRT-PCR were used to detect the ability of bioactive factors in CM to migrate to h MSCs, differentiate into adipocytes and osteoblasts, and senescence. The effect of microRNA expression. Results the bioactive factor of CM derived from young h MSCs could promote the functional regeneration of MSCs, enhance the ability of migration and differentiation into adipocytes and osteoblasts, and reduce senescence. On the contrary, the young h MSCs was placed in the aged h MSCs source CM, the function of the young h MSCs was impaired, the migration ability was decreased, the differentiation ability to adipocytes and osteoblasts was decreased, and the senescence was increased. The bioactive factor of CM derived from young h MSCs could promote the expression of miR-10a in h MSCs, but the bioactive factor derived from CM from old h MSCs could inhibit its expression. Conclusion the bioactive factor derived from young h MSCs can promote the expression of miR-10a, promote the migration and differentiation of h MSCs, reduce cell senescence and promote the functional regeneration of h MSCs.
【作者單位】： 廣州醫(yī)科大學附屬第二醫(yī)院廣州心血管疾病研究所;
【基金】：國家自然科學基金資助項目(編號:81401156) 廣州市醫(yī)藥衛(wèi)生科技項目(編號:20141A011085)
【分類號】：R329
【參考文獻】
相關期刊論文 前2條
1 黎佼;陳敏生;楊偉健;張振輝;鐘峗;劉世明;;年輕骨髓間充質(zhì)干細胞可通過細胞融合改善年老骨髓間充質(zhì)干細胞功能[J];中國病理生理雜志;2010年05期
2 黎佼;董s，
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