【摘要】：目的在新西蘭兔腰5椎體下終板種植結(jié)核分枝桿菌H37Rv標(biāo)準(zhǔn)菌株,構(gòu)建兔脊柱結(jié)核模型。方法65只新西蘭兔隨機(jī)分為3組,實(shí)驗(yàn)組30只,對(duì)照組30只,空白對(duì)照組5只。弗氏完全佐劑致敏1月后,在腰5椎體下終板鉆孔,實(shí)驗(yàn)組注入1×107CFU·m L~(-1)結(jié)核桿菌混懸液0.1m L,對(duì)照組注入等量生理鹽水,空白對(duì)照組不作任何處理。術(shù)后觀察兔一般情況,術(shù)后1個(gè)月、2個(gè)月對(duì)造模兔行X線、CT檢查,術(shù)后2個(gè)月處死動(dòng)物,行大體解剖觀察,病椎取材作石蠟切片,對(duì)CT為陽性表現(xiàn)的兔椎旁肉芽組織進(jìn)行結(jié)核桿菌培養(yǎng)。結(jié)果實(shí)驗(yàn)組25只、對(duì)照組28只,空白對(duì)照組5只兔完成實(shí)驗(yàn)。X線檢查:術(shù)后1個(gè)月,實(shí)驗(yàn)組36.0%(9/25)的兔腰5、6椎體出現(xiàn)輕度骨質(zhì)破壞;術(shù)后2個(gè)月,實(shí)驗(yàn)組52.0%(13/25)的兔腰5、6椎體骨質(zhì)破壞較前加重,1只出現(xiàn)脊柱后凸畸形;對(duì)照組未見椎體骨質(zhì)破壞,椎間隙無變化。CT掃描:術(shù)后1個(gè)月,實(shí)驗(yàn)組48.0%(12/25)的兔腰5、6椎體骨質(zhì)破壞,病椎內(nèi)細(xì)小死骨形成;術(shù)后2個(gè)月,實(shí)驗(yàn)組72.0%(18/25)的兔腰5、6椎體骨質(zhì)破壞較前加重,5只椎旁膿腫形成;對(duì)照組未見椎體骨質(zhì)破壞,無椎旁膿腫形成。術(shù)后2個(gè)月,實(shí)驗(yàn)組18只病椎石蠟切片蘇木素-伊紅(HE)染色顯示有類上皮樣細(xì)胞、結(jié)核壞死灶形成。結(jié)合影像學(xué)與組織病理學(xué),術(shù)后2個(gè)月,兔脊柱結(jié)核造模成功率為72.0%(18/25)。18只兔的椎旁肉芽組織(CT陽性表現(xiàn))結(jié)核桿菌培養(yǎng)陽性率為38.9%(7/18)。結(jié)論弗氏完全佐劑致敏的新西蘭兔腰5椎體局部種植H37Rv標(biāo)準(zhǔn)菌株,可成功構(gòu)建兔脊柱結(jié)核模型。
[Abstract]:Objective to establish a spinal tuberculosis model in New Zealand rabbits by planting Mycobacterium tuberculosis H37Rv standard strain in the lower lumbar endplate of 5 vertebrae. Methods Sixty-five New Zealand rabbits were randomly divided into 3 groups: experimental group (n = 30), control group (n = 30) and blank control group (n = 5). One month after sensitization with Freund's complete adjuvant, 1 脳 107CFU ml ~ (-1) Mycobacterium tuberculosis suspension was injected into the lumbar 5 vertebrae lower endplate in the experimental group, the control group was injected with the same amount of normal saline, and the blank control group was not treated. After 1 month and 2 months after operation, the model rabbits were examined by X-ray and CT. The animals were killed 2 months after operation, the animals were observed by general anatomy, and the diseased vertebrae were taken as paraffin sections. Mycobacterium tuberculosis was cultured in paravertebral granulation tissue of rabbits with positive CT. Results 25 rabbits in the experimental group, 28 rabbits in the control group and 5 rabbits in the blank control group completed the experiment. X-ray examination showed that 36.0% (9 / 25) of the rabbits in the experimental group had slight bone destruction in the lumbar vertebrae of the experimental group 1 month after operation. 2 months after operation, 52.0% (13 / 25) of the rabbits in the experimental group suffered more serious bony destruction in the lumbar vertebrae, and 1 had kyphosis deformity. CT scan: 1 month after operation, 48.0% (12 / 25) of the rabbits' lumbar vertebrae in the experimental group suffered from bone destruction, and small dead bones were formed in the diseased vertebrae. Two months after operation, 72.0% (18 / 25) of the rabbits in the experimental group had more serious bone destruction and 5 paravertebral abscesses in the lumbar spine of the experimental group than before, while in the control group, there was no bony destruction and no paravertebral abscess. At 2 months after operation, hematoxylin-eosin (HE) staining in paraffin sections of 18 diseased vertebrae in the experimental group showed epithelioid cells and necrotic tuberculous foci. Combined with imaging and histopathology, the success rate of spinal tuberculosis modeling was 72.0% (18 / 25) at 2 months after operation, and the positive rate of Mycobacterium tuberculosis culture in paravertebral granulation (CT) was 38.9% (7 / 18) in 18 rabbits. Conclusion the H37Rv standard strain in the lumbar 5 vertebrae of New Zealand rabbits sensitized with Freund's complete adjuvant can successfully construct the spinal tuberculosis model in rabbits.
【作者單位】： 寧夏醫(yī)科大學(xué)總醫(yī)院骨科;寧夏醫(yī)科大學(xué);寧夏醫(yī)科大學(xué)總醫(yī)院醫(yī)學(xué)檢驗(yàn)中心;
【基金】：國家自然科學(xué)基金(30960391)
【分類號(hào)】：R529.2;R-332

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