【摘要】： 人巨細(xì)胞病毒UL 146基因—α趨化因子在巨細(xì)胞病毒感染中作用的研究 目的 研究人巨細(xì)胞病毒(HCMV)UL146基因在巨細(xì)胞病毒感染臨床株中的多態(tài)性,尋找具有臨床診斷、治療價(jià)值的基因及基因的變化,為HCMV感染的臨床診斷和防治提供資料。 方法 選擇經(jīng)QPCR檢測尿HCMV-DNA為陽性的臨床株進(jìn)行UL146基因序列PCR擴(kuò)增,對擴(kuò)增陽性標(biāo)本進(jìn)行序列測定和分析。 結(jié)果 28株擴(kuò)增陽性標(biāo)本序列之間有很大變異,種系樹化分析結(jié)果顯示可以分為3個(gè)型,4個(gè)亞型,G1組氨基序列與Toledo株的同源性為96.6%-98.3%,G2組為10.3%-22.2%,G3組為12.8%-13.7%；ELRCXC的趨化因子功能區(qū)在各序列中高度保守。 結(jié)論 HCMV-UL146基因呈現(xiàn)高度多態(tài)性,其編碼的ELRCXC趨化因子功能區(qū)在各序列中高度保守,HCMV-UL146基因多態(tài)性與HCMV感染不同臨床癥狀之間無明顯的關(guān)系。
[Abstract]:Study on the role of human cytomegalovirus UL 146 gene 偽 chemokine in cytomegalovirus infection objective to study the polymorphism of human cytomegalovirus (HCMV) UL146 gene in clinical cytomegalovirus infection. To search for the gene and gene changes with clinical diagnosis and therapeutic value, and to provide data for clinical diagnosis and prevention and treatment of HCMV infection. Methods the UL146 gene sequence was amplified by PCR from clinical strains which were positive for urinary HCMV-DNA detected by QPCR. The sequence of positive samples was determined and analyzed. Results there was great variation among 28 amplified positive specimens. The results of phylogenetic analysis showed that there were 3 types and 4 subtypes. The amino sequence of G1 group was homologous to Toledo strain, the homology was 96.6- 98.3%. In G2 group, 10.3- 22.2and G3 were 12.8- 13.7; The chemokine functional regions of ELRCXC are highly conserved in each sequence. Conclusion HCMV-UL146 gene is highly polymorphic and its ELRCXC chemokine functional region is highly conserved in each sequence. There is no significant relationship between HCMV-UL146 gene polymorphism and different clinical symptoms of HCMV infection.
【學(xué)位授予單位】：中國醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R373

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