【摘要】：目的：研究低強(qiáng)度紫外線UVB（Ultraviolet B，280-315nm）照射HaCaT細(xì)胞后，對(duì)HaCaT細(xì)胞凋亡和增殖的影響及其影響Akt信號(hào)通路的潛在機(jī)制。 方法：3mJ/cm2、5mJ/cm2和7mJ/cm2強(qiáng)度的UVB分別照射HaCaT細(xì)胞18，36和72小時(shí)后，收集細(xì)胞進(jìn)行細(xì)胞凋亡和增殖檢測(cè)；選取5mJ/cm2強(qiáng)度的UVB分別照射HaCaT細(xì)胞18小時(shí)后，收集細(xì)胞進(jìn)行增殖檢測(cè)；提取照射后HaCaT細(xì)胞的總蛋白進(jìn)行免疫印跡實(shí)驗(yàn)，檢測(cè)Akt，Bad和Bad-p136的表達(dá)情況。 結(jié)果：3mJ/cm2、5mJ/cm2和7mJ/cm2強(qiáng)度的UVB照射HaCaT細(xì)胞18，36和72小時(shí)后均不影響HaCaT細(xì)胞的凋亡水平。5mJ/cm2和7mJ/cm2強(qiáng)度的UVB照射HaCaT細(xì)胞18和36小時(shí)后不影響HaCaT細(xì)胞的增殖能力，而5mJ/cm2和7mJ/cm2強(qiáng)度的UVB照射72小時(shí)后可以顯著促進(jìn)HaCaT細(xì)胞的增殖。免疫印跡實(shí)驗(yàn)證實(shí)5mJ/cm2UVB照射后18小時(shí)后，，Bad的表達(dá)量基本維持正常，而AKT和Bad-p136表達(dá)水平顯著升高。 結(jié)論：3mJ/cm2、5mJ/cm2和7mJ/cm2強(qiáng)度的UVB照射不影響細(xì)胞的凋亡水平，然而長(zhǎng)時(shí)間5mJ/cm-2強(qiáng)度的UVB照射能促進(jìn)HaCaT細(xì)胞的增殖，且這種作用可能是通過(guò)Akt信號(hào)通路進(jìn)行調(diào)節(jié)，這可能是紫外線照射相關(guān)皮膚腫瘤發(fā)生發(fā)展的潛在危險(xiǎn)因素。
[Abstract]:Aim: to study the effects of low-intensity ultraviolet UVB (Ultraviolet Bon 280-315 nm irradiation on apoptosis and proliferation of HaCaT cells and the potential mechanism of affecting Akt signaling pathway. Methods: HaCaT cells were irradiated with 3mJ / cm2 5mJ / cm2 and UVB with 7mJ/cm2 intensity for 1836 hours and 72 hours, respectively. Cell apoptosis and proliferation were detected. After the HaCaT cells were irradiated with UVB with 5mJ/cm2 intensity for 18 hours, the cells were collected for proliferation detection, and the total protein of HaCaT cells was extracted for Western blot assay to detect the expression of Akt,Bad and Bad-p136. Results: the apoptosis level of HaCaT cells was not affected by 3mJ / cm2 and 7mJ/cm2 intensity UVB irradiation for 1836 and 72 hours, but the proliferation ability of HaCaT cells was not affected by 5mJ/cm2 and 7mJ/cm2 UVB irradiation on HaCaT cells for 18 and 36 hours. The proliferation of HaCaT cells was significantly promoted by 5mJ/cm2 and 7mJ/cm2 intensive UVB irradiation for 72 hours. Western blot analysis showed that the expression of Bad remained normal after 18 hours of 5mJ/cm2UVB irradiation, while the expression of AKT and Bad-p136 increased significantly. Conclusion: UVB irradiation with 3mJ / cm2 and 7mJ/cm2 intensity does not affect the apoptosis level of HaCaT cells. However, UVB irradiation with 5mJ/cm-2 intensity for a long time can promote the proliferation of HaCaT cells, and this effect may be regulated by Akt signaling pathway. This may be a potential risk factor for the development of skin tumors associated with ultraviolet radiation.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R329.2

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 王淑安;潘建玲;孫瑞;;環(huán)氧化酶-2的表達(dá)與皮膚鱗狀細(xì)胞癌的關(guān)系[J];哈爾濱醫(yī)藥;2007年03期

2 朱東寧;簡(jiǎn)強(qiáng);薛柯;劉博強(qiáng);王玲;李承新;;UVB照射對(duì)PIG1細(xì)胞增殖活性的影響[J];中國(guó)美容醫(yī)學(xué);2011年06期

本文編號(hào)：2410125