【摘要】：目的建立肝郁脾虛型腸易激綜合征(IBS)模型組和對(duì)照組結(jié)腸組織蛋白質(zhì)雙向電泳圖譜,結(jié)合質(zhì)譜技術(shù)鑒定并分析差異表達(dá)蛋白,對(duì)候選表達(dá)差異蛋白進(jìn)行蛋白質(zhì)功能和相關(guān)研究,進(jìn)一步闡明肝郁脾虛型IBS病理機(jī)制。方法采用雙向凝膠電泳法分離IBS模型組和正常對(duì)照組結(jié)腸粘膜差異蛋白,運(yùn)用MALDI TOF/TOF串聯(lián)飛行時(shí)間質(zhì)譜儀進(jìn)行鑒定,運(yùn)用Gene Ontology和Pathway Studio生物信息學(xué)方法將所鑒定的差異蛋白進(jìn)行功能分類,分析IBS病理機(jī)制中關(guān)鍵蛋白。結(jié)果首次運(yùn)用蛋白組學(xué)的方法尋找IBS模型組和正常對(duì)照組結(jié)腸的差異表達(dá)蛋白特征譜,結(jié)果發(fā)現(xiàn)差異倍數(shù)在2.5倍以上的差異蛋白有15個(gè),其中2個(gè)蛋白表達(dá)上調(diào),13個(gè)蛋白表達(dá)下調(diào)。結(jié)論熱休克蛋白、硫氧還蛋白、氯離子通道蛋白等可能是肝郁脾虛型IBS病理機(jī)制中潛在分子標(biāo)志物。
[Abstract]:Objective to establish a two-dimensional electrophoresis map of colon protein in (IBS) model group and control group of irritable bowel syndrome with liver stagnation and spleen deficiency, and to identify and analyze the differentially expressed proteins by mass spectrometry. Protein function and related studies of candidate differentially expressed proteins were carried out to further elucidate the pathological mechanism of IBS with liver stagnation and spleen deficiency. Methods differential proteins of colon mucosa in IBS model group and normal control group were separated by two dimensional gel electrophoresis and identified by MALDI TOF/TOF series time of flight mass spectrometer. The differential proteins were classified by Gene Ontology and Pathway Studio bioinformatics, and the key proteins in the pathological mechanism of IBS were analyzed. Results the differential expression protein characteristic profiles of IBS model group and normal control group were searched by proteomics for the first time. The results showed that 15 differentially expressed proteins were more than 2.5 times different, 2 of which were up-regulated. 13 proteins were down-regulated. Conclusion Heat shock protein, thioredoxin and chloride channel protein may be potential molecular markers in the pathogenesis of IBS with liver stagnation and spleen deficiency.
【作者單位】： 華南腫瘤學(xué)國家重點(diǎn)實(shí)驗(yàn)室/中山大學(xué)附屬腫瘤醫(yī)院藥學(xué)部;廣州中醫(yī)藥大學(xué)第一附屬醫(yī)院藥學(xué)部;
【基金】：國家自然科學(xué)基金(No.81073160) 廣東省自然科學(xué)基金(No.S2012010010539)
【分類號(hào)】：R-332;R228

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本文編號(hào)：2405930