發(fā)布時間：2019-01-09 18:15

【摘要】：目的建立肝郁脾虛型腸易激綜合征(IBS)模型組和對照組結(jié)腸組織蛋白質(zhì)雙向電泳圖譜,結(jié)合質(zhì)譜技術(shù)鑒定并分析差異表達蛋白,對候選表達差異蛋白進行蛋白質(zhì)功能和相關(guān)研究,進一步闡明肝郁脾虛型IBS病理機制。方法采用雙向凝膠電泳法分離IBS模型組和正常對照組結(jié)腸粘膜差異蛋白,運用MALDI TOF/TOF串聯(lián)飛行時間質(zhì)譜儀進行鑒定,運用Gene Ontology和Pathway Studio生物信息學方法將所鑒定的差異蛋白進行功能分類,分析IBS病理機制中關(guān)鍵蛋白。結(jié)果首次運用蛋白組學的方法尋找IBS模型組和正常對照組結(jié)腸的差異表達蛋白特征譜,結(jié)果發(fā)現(xiàn)差異倍數(shù)在2.5倍以上的差異蛋白有15個,其中2個蛋白表達上調(diào),13個蛋白表達下調(diào)。結(jié)論熱休克蛋白、硫氧還蛋白、氯離子通道蛋白等可能是肝郁脾虛型IBS病理機制中潛在分子標志物。
[Abstract]:Objective to establish a two-dimensional electrophoresis map of colon protein in (IBS) model group and control group of irritable bowel syndrome with liver stagnation and spleen deficiency, and to identify and analyze the differentially expressed proteins by mass spectrometry. Protein function and related studies of candidate differentially expressed proteins were carried out to further elucidate the pathological mechanism of IBS with liver stagnation and spleen deficiency. Methods differential proteins of colon mucosa in IBS model group and normal control group were separated by two dimensional gel electrophoresis and identified by MALDI TOF/TOF series time of flight mass spectrometer. The differential proteins were classified by Gene Ontology and Pathway Studio bioinformatics, and the key proteins in the pathological mechanism of IBS were analyzed. Results the differential expression protein characteristic profiles of IBS model group and normal control group were searched by proteomics for the first time. The results showed that 15 differentially expressed proteins were more than 2.5 times different, 2 of which were up-regulated. 13 proteins were down-regulated. Conclusion Heat shock protein, thioredoxin and chloride channel protein may be potential molecular markers in the pathogenesis of IBS with liver stagnation and spleen deficiency.
【作者單位】： 華南腫瘤學國家重點實驗室/中山大學附屬腫瘤醫(yī)院藥學部;廣州中醫(yī)藥大學第一附屬醫(yī)院藥學部;
【基金】：國家自然科學基金(No.81073160) 廣東省自然科學基金(No.S2012010010539)
【分類號】：R-332;R228

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