【摘要】： 目的:分析1型糖尿病患者外周血漿細胞樣樹突狀細胞(PDC)數量及功能變化。 方法:采用流式細胞分析技術檢測22例1型糖尿病患者(T1DM)及20例健康人體內PDC細胞的比例及數量;用免疫刺激序列(CpG ODN-2216)刺激外周血單個核細胞(PBMCs)并與PBMCs共培養(yǎng),通過檢測PBMCs產生的IFN-α水平評估PDC的功能。 結果:與健康對照組比較,1型糖尿病患者組外周血PDC的百分比和數量均顯著下降(p＜0.01);PDC經CpG ODN-2216刺激后產生IFN-α的能力較健康對照組也有顯著下降(p＜0.05);相關性分析表明,經CpG ODN-2216刺激后IFN-α的產量與PDC數量呈正相關關系(r=0.439,p＜0.05)。 結論:1型糖尿病患者體內PDC數量和功能異常,說明PDC可能積極參與了患者機體的免疫紊亂過程;PDC產生的IFN-α減少,可能使患者對感染的控制能力降低而易感。
[Abstract]:Objective: to analyze the changes of (PDC) number and function of plasma cell like dendritic cells (PBMC) in type 1 diabetes mellitus (DM). Methods: the proportion and quantity of PDC cells in 22 patients with type 1 diabetes mellitus (T1DM) and 20 healthy people were detected by flow cytometry. Peripheral blood mononuclear cells (PBMCs) were stimulated by immunostimulatory sequence (CpG ODN-2216) and co-cultured with PBMCs. The function of PDC was evaluated by detecting the level of IFN- 偽 produced by PBMCs. Results: compared with the healthy control group, the percentage and quantity of PDC in peripheral blood of type 1 diabetic patients decreased significantly (p < 0. 01). The ability of PDC stimulated by CpG ODN-2216 to produce IFN- 偽 was significantly lower than that of control group (p < 0. 05). Correlation analysis showed that the yield of IFN- 偽 was positively correlated with the quantity of PDC after CpG ODN-2216 stimulation (r = 0. 439, p < 0. 05). Conclusion: the abnormal number and function of PDC in patients with type 1 diabetes mellitus indicate that PDC may participate in the immune disorder process of the patients, and the decrease of IFN- 偽 produced by PDC may make the patients' ability to control infection lower and susceptible to infection.
【學位授予單位】：廣西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2008
【分類號】：R392;R587.1

【參考文獻】

相關期刊論文 前6條

1 張永亮,曹雪濤;漿細胞樣樹突狀細胞免疫調節(jié)功能研究進展[J];現代免疫學;2005年02期

2 張程亮;向明;鄒曉蕾;彭佳蓓;;髓源性樹突狀細胞過繼轉移誘導1型糖尿病小鼠免疫耐受及其機制[J];世界華人消化雜志;2006年33期

3 賈慶哲;葛均波;梁春;羅育坤;黃東;王克強;陳灝珠;;糖基化終產物對人單核細胞源樹突狀細胞糖基化終產物受體表達的研究[J];中國病理生理雜志;2006年01期

4 段學章,李捍衛(wèi),王敏,王福生;乙型肝炎病毒感染者樹突狀細胞(DC)亞群的變化及意義[J];中華微生物學和免疫學雜志;2004年11期

5 姚康;葛均波;孫愛軍;洪曉武;施鴻毓;黃榕，

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