【摘要】：目的：本實驗通過檢測鹽酸/硫酸氨基葡萄糖治療兔膝骨關(guān)節(jié)炎時關(guān)節(jié)液中IL-1和TNF-α含量來探討鹽酸/硫酸氨基葡萄糖(glucosamine hydrochloride,OTL/glucosamine sulfate,WGL)對骨性關(guān)節(jié)炎的作用及機制。 方法：選取健康雄性成年大白兔36只,體重2.0-2.5kg。隨機分為3組(n=12)：對照組(A組)、鹽酸氨基葡萄糖組(B組)、硫酸氨基葡萄糖組(C組)。三組均按改良Hulth法制備膝骨性關(guān)節(jié)炎模型；B、C組于造模術(shù)后第2開始每天分別灌胃鹽酸/硫酸氨基葡萄糖(90mg/kg),連續(xù)8周；A組自由飲食。傷口愈合后每天驅(qū)趕所有兔子行走2次,30min/次。給藥8周后拍攝對照組兔子雙側(cè)膝關(guān)節(jié)DR片,對比雙側(cè)膝關(guān)節(jié)影像學(xué)變化；空氣栓塞法處死所有實驗兔,收集右膝關(guān)節(jié)液,用ELISA法檢測關(guān)節(jié)液中IL-1和TNF-α含量；解剖對照組兔雙側(cè)膝關(guān)節(jié),觀察雙側(cè)股骨髁肉眼改變并評分。 結(jié)果：實驗動物全部存活,傷口愈合良好。對照組(A組)雙側(cè)膝關(guān)節(jié)DR片對比可見右膝關(guān)節(jié)周軟組織腫脹較明顯,內(nèi)側(cè)關(guān)節(jié)間隙狹窄,呈膝骨性關(guān)節(jié)炎改變。肉眼觀A組右股骨髁軟骨面剝脫、缺損嚴(yán)重,可見軟骨下骨質(zhì)外露,以股骨內(nèi)側(cè)髁為重；A組左側(cè)股骨髁軟骨面光滑、色澤正常；雙側(cè)膝關(guān)節(jié)軟骨評分有統(tǒng)計學(xué)差異(P0.05)。三組關(guān)節(jié)液中均可檢測出IL-1和TNF-α,與A組比較,B組與C組關(guān)節(jié)液中IL-1和TNF-α均下降,有統(tǒng)計學(xué)意義(P0.05),B組與C組比較無明顯差異(P0.05)。 結(jié)論：改良Hulth造模方法可以制備出膝骨性關(guān)節(jié)炎模型；鹽酸氨基葡萄糖與硫酸氨基葡萄糖可能通過抑制關(guān)節(jié)液中炎性因子IL-1和TNF-α的表達(dá),延緩了軟骨病變的速度,鹽酸氨基葡萄糖與硫酸氨基葡萄糖無明顯差異。
[Abstract]:Objective: to investigate the content of IL-1 and TNF- 偽 in articular fluid of rabbit knee osteoarthritis treated with hydrochloric acid / glucosamine sulfate (glucosamine hydrochloride,OTL/glucosamine sulfate,). The effect and mechanism of WGL on osteoarthritis. Methods: 36 healthy male adult white rabbits were selected, weighing 2.0-2.5 kg. They were randomly divided into 3 groups: control group (group A), glucosamine hydrochloride group (group B) and glucosamine sulfate group (group C). The knee osteoarthritis model was established by modified Hulth method in all the three groups, the rats in group C were given hydrochloric acid / glucosamine sulfate (90mg/kg) daily for 8 weeks, group A was fed with free diet. After wound healing, all rabbits were driven to walk twice a day, 30min/. After 8 weeks of administration, the DR film of bilateral knee joint of the control group was taken and the imaging changes of bilateral knee joint were compared. All the experimental rabbits were killed by air embolization, and the contents of IL-1 and TNF- 偽 in the joint fluid were detected by ELISA method, and the right knee joint fluid was collected. The bilateral knee joint of the control group was dissected and the changes of bilateral femoral condyle were observed and scored. Results: all the experimental animals survived and the wound healed well. In the control group (group A), the soft tissue swelling around the right knee joint was obvious, the medial joint space was narrow, and the knee osteoarthritis was observed on DR film of bilateral knee joint. In group A, the right femoral condyle cartilage was dissected, the defect was serious, the subchondral bone was exposed, the medial femoral condyle was the most serious, the left femoral condylar cartilage in group A was smooth and the color was normal. Bilateral knee cartilage score was statistically different (P0.05). IL-1 and TNF- 偽 were detected in all three groups. Compared with group A, IL-1 and TNF- 偽 in group B and group C decreased significantly (P0.05). There was no significant difference between group), B and group C (P0.05). Conclusion: the model of knee osteoarthritis can be made by modified Hulth method. Glucosamine hydrochloride and glucosamine sulfate may inhibit the expression of inflammatory factors IL-1 and TNF- 偽 in articular fluid, and delay the speed of cartilage lesion. There is no significant difference between glucosamine hydrochloride and glucosamine sulfate.
【學(xué)位授予單位】：遵義醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R-332;R684.3;R965

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