丙二醛及晚期糖基化終產(chǎn)物在健康成人血清與唾液中的增齡性變化及相關(guān)性研究
發(fā)布時(shí)間：2018-12-17 23:34
【摘要】： 我國(guó)已經(jīng)進(jìn)入老齡化社會(huì),防衰抗老成為研究的熱點(diǎn)。衰老可分為兩種：生理性衰老和病理性衰老。生理性衰老是指生物體隨著時(shí)間的推移,逐漸發(fā)生的退行性變化而引起的代謝功能的下降,從而出現(xiàn)的衰老狀態(tài)。病理性衰老是指由各種慢性疾病引起的器官退行性變化。因此尋求客觀的反應(yīng)機(jī)體生理性衰老的生物學(xué)標(biāo)志物,成為定量的分析個(gè)體生理性衰老的前提。目前研究者主要是從細(xì)胞水平、分子水平研究衰老生物學(xué)標(biāo)志。氧化應(yīng)激衰老理論和非酶糖基化衰老理論是目前研究的較多的衰老理論。丙二醛(malondialdehyde, MDA)是脂質(zhì)過(guò)氧化反應(yīng)的產(chǎn)物,晚期糖基化終產(chǎn)物(advanced glycosylation end products, AGEs)是非酶糖基化反應(yīng)的終產(chǎn)物。應(yīng)用唾液中的某些成分來(lái)檢測(cè)疾病越來(lái)越得到人們的重視,唾液檢測(cè)與血液相比取樣方便,無(wú)創(chuàng)傷,患者易于接受。以往的研究主要是測(cè)定血液、組織中的MDA、AGEs與衰老的關(guān)系,那么是否能通過(guò)測(cè)定唾液中的含量而檢測(cè)人體的衰老程度呢?關(guān)于唾液中檢測(cè)健康人的MDA、AGEs的增齡性變化尚未見(jiàn)報(bào)導(dǎo)。因此本實(shí)驗(yàn)研究唾液中的MDA、AGEs含量的隨齡變化,探討唾液是否能代替血液成為測(cè)定衰老的生物學(xué)指標(biāo)。 目的：研究不同年齡段健康成人血清、唾液中的MDA、AGEs的隨齡變化,探討其在血清和唾液中的含量與年齡的相關(guān)性,及在血清和唾液中含量的相關(guān)性,為進(jìn)一步研究唾液中衰老的生物學(xué)指標(biāo)提供理論依據(jù)。 方法：健康成人120名,分為20-39歲、40-59歲、60-79歲、80歲以上4組,每組30人�？崭谷§o脈血及唾液,用硫代巴比妥酸比色法、酶聯(lián)免疫吸附試驗(yàn),分別測(cè)定血清和唾液中的MDA、AGEs的含量。 結(jié)果：1.血清和唾液中MDA含量：(1)60-79歲組及80以上組血清、唾液中MDA的含量顯著高于20-39歲與40-59歲組(P0.05),80歲以上組血清和唾液中MDA的含量與60-79歲組的含量無(wú)顯著性差異(p0.05)；40-59歲組健康人血清、唾液中MDA的含量與20-39歲組健康人血清和唾液中MDA的含量無(wú)統(tǒng)計(jì)學(xué)差異(p0.05)。(2)唾液與血清中MDA的含量存在正相關(guān)性,(r=0.79,p0.01)。2.血清和唾液中AGEs含量：(1)80歲以上組血清和唾液中AGEs的含量明顯高于其它各組,有統(tǒng)計(jì)學(xué)意義(p0.05)；40-59歲組、60-79歲組血清和唾液中的AGEs的含量顯著高于20-39歲組,差異有統(tǒng)計(jì)學(xué)意義(p0.05)；40-59歲組與60-79歲組血清和唾液中AGEs的含量無(wú)統(tǒng)計(jì)學(xué)差異(p0.05)。 (2)唾液與血清中AGEs的含量存在正相關(guān)性,(r=0.90,p0.01)。 結(jié)論：(1)血清和唾液中的MDA、AGEs的含量隨著年齡的增長(zhǎng)而升高； (2)唾液中MDA、AGEs水平有望代替血清中的MDA、AGEs水平成為衰老的生物學(xué)指標(biāo)之一。
[Abstract]:China has entered an aging society, anti-aging and anti-aging has become the focus of research. Senescence can be divided into two types: physiological senescence and pathological senescence. Physiological senescence refers to the decline of metabolic function caused by the degenerative changes of organisms over time. Pathological aging refers to the degenerative changes of organs caused by various chronic diseases. Therefore, seeking an objective biomarker of physiological senescence is the premise of quantitative analysis of individual physiological senescence. At present, researchers mainly study the biological markers of senescence at the cell level and molecular level. Oxidative stress senescence theory and non-enzymatic glycosylation theory are many aging theories. Malondialdehyde (malondialdehyde, MDA) is the product of lipid peroxidation, and the late glycation end product (advanced glycosylation end products, AGEs) is the end product of non-enzymatic glycosylation. More and more attention has been paid to the use of saliva to detect diseases. Compared with blood, saliva detection is more convenient, non-invasive and easy to accept by patients. Previous studies have focused on measuring the relationship between MDA,AGEs in blood and tissue and aging. Can we measure the extent of human aging by measuring the content of saliva? The age-related changes of MDA,AGEs in saliva for healthy people have not been reported. In this study, we studied the changes of MDA,AGEs content in saliva with age, and explored whether saliva could replace blood as a biological index for the determination of aging. Objective: to study the changes of MDA,AGEs in serum and saliva of healthy adults of different ages, and to explore the correlation between the content of MDA,AGEs in serum and saliva and age, and the content of MDA,AGEs in serum and saliva. To provide theoretical basis for further study of biological indicators of saliva aging. Methods: 120 healthy adults were divided into 4 groups: 20-39 years old, 40-59 years old, 60-79 years old and 80 years old. Venous blood and saliva were collected on an empty stomach. The MDA,AGEs content in serum and saliva was determined by thiobarbituric acid colorimetry and enzyme-linked immunosorbent assay (Elisa). Results: 1. The content of MDA in serum and saliva: (1) the content of MDA in saliva of 60-79 years old group and over 80 years group was significantly higher than that of 20-39 and 40-59 years old group (P0.05). The content of MDA in serum and saliva of the group over 80 years old was not significantly different from that of the group aged 60-79 years (p0.05). There was no significant difference between the MDA content in saliva and the MDA content in serum and saliva of healthy subjects aged 40 to 59 years old (p0.05). (2). There was a positive correlation between the content of MDA in saliva and that in serum (r = 0.79, r = 0.79). P0.01). The content of AGEs in serum and saliva: (1) the content of AGEs in serum and saliva of the group over 80 years old was significantly higher than that of the other groups (p0.05); The levels of AGEs in serum and saliva of 40-59 years old group and 60-79 years old group were significantly higher than those of 20-39 years old group (p0.05). There was no significant difference in AGEs in serum and saliva between 40 and 59 years old group and 60-79 years old group (p 0.05). (2) there was a positive correlation between saliva and serum AGEs content (r = 0.90, p 0.01). Conclusion: (1) the content of MDA,AGEs in serum and saliva increases with age, (2) the level of MDA,AGEs in saliva may replace the level of MDA,AGEs in serum as one of the biological indexes of aging.
【學(xué)位授予單位】：中國(guó)人民解放軍軍醫(yī)進(jìn)修學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類(lèi)號(hào)】：R363
本文編號(hào)：2384951
[Abstract]:China has entered an aging society, anti-aging and anti-aging has become the focus of research. Senescence can be divided into two types: physiological senescence and pathological senescence. Physiological senescence refers to the decline of metabolic function caused by the degenerative changes of organisms over time. Pathological aging refers to the degenerative changes of organs caused by various chronic diseases. Therefore, seeking an objective biomarker of physiological senescence is the premise of quantitative analysis of individual physiological senescence. At present, researchers mainly study the biological markers of senescence at the cell level and molecular level. Oxidative stress senescence theory and non-enzymatic glycosylation theory are many aging theories. Malondialdehyde (malondialdehyde, MDA) is the product of lipid peroxidation, and the late glycation end product (advanced glycosylation end products, AGEs) is the end product of non-enzymatic glycosylation. More and more attention has been paid to the use of saliva to detect diseases. Compared with blood, saliva detection is more convenient, non-invasive and easy to accept by patients. Previous studies have focused on measuring the relationship between MDA,AGEs in blood and tissue and aging. Can we measure the extent of human aging by measuring the content of saliva? The age-related changes of MDA,AGEs in saliva for healthy people have not been reported. In this study, we studied the changes of MDA,AGEs content in saliva with age, and explored whether saliva could replace blood as a biological index for the determination of aging. Objective: to study the changes of MDA,AGEs in serum and saliva of healthy adults of different ages, and to explore the correlation between the content of MDA,AGEs in serum and saliva and age, and the content of MDA,AGEs in serum and saliva. To provide theoretical basis for further study of biological indicators of saliva aging. Methods: 120 healthy adults were divided into 4 groups: 20-39 years old, 40-59 years old, 60-79 years old and 80 years old. Venous blood and saliva were collected on an empty stomach. The MDA,AGEs content in serum and saliva was determined by thiobarbituric acid colorimetry and enzyme-linked immunosorbent assay (Elisa). Results: 1. The content of MDA in serum and saliva: (1) the content of MDA in saliva of 60-79 years old group and over 80 years group was significantly higher than that of 20-39 and 40-59 years old group (P0.05). The content of MDA in serum and saliva of the group over 80 years old was not significantly different from that of the group aged 60-79 years (p0.05). There was no significant difference between the MDA content in saliva and the MDA content in serum and saliva of healthy subjects aged 40 to 59 years old (p0.05). (2). There was a positive correlation between the content of MDA in saliva and that in serum (r = 0.79, r = 0.79). P0.01). The content of AGEs in serum and saliva: (1) the content of AGEs in serum and saliva of the group over 80 years old was significantly higher than that of the other groups (p0.05); The levels of AGEs in serum and saliva of 40-59 years old group and 60-79 years old group were significantly higher than those of 20-39 years old group (p0.05). There was no significant difference in AGEs in serum and saliva between 40 and 59 years old group and 60-79 years old group (p 0.05). (2) there was a positive correlation between saliva and serum AGEs content (r = 0.90, p 0.01). Conclusion: (1) the content of MDA,AGEs in serum and saliva increases with age, (2) the level of MDA,AGEs in saliva may replace the level of MDA,AGEs in serum as one of the biological indexes of aging.
【學(xué)位授予單位】：中國(guó)人民解放軍軍醫(yī)進(jìn)修學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類(lèi)號(hào)】：R363
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