水浸應(yīng)激型胃潰瘍寒熱證模型的建立與相關(guān)性方劑對其影響的研究
發(fā)布時間：2018-11-21 11:01
【摘要】： 目的：將水浸應(yīng)激型胃潰瘍疾病模型與中醫(yī)寒、熱證相結(jié)合，探討應(yīng)激型潰瘍寒、熱證形成機(jī)制，揭示應(yīng)激型潰瘍寒、熱證候?qū)嵸|(zhì)；觀察寒性方劑大黃黃連瀉心湯、熱性方劑理中丸對應(yīng)激型潰瘍寒、熱證模型的作用機(jī)理及方證相應(yīng)規(guī)律。 方法：采用寒因素(冰水、0．3mol／L氫氧化鈉)及熱因素(8％辣椒粉、60％乙醇)建立胃寒、胃熱證模型，結(jié)合國內(nèi)外較為公認(rèn)的束縛水浸應(yīng)激法創(chuàng)建應(yīng)激型潰瘍寒、熱證結(jié)合模型，寒性方劑大黃黃連瀉心湯、熱性方劑理中丸灌胃治療。1、束縛水浸應(yīng)激7h后處死各模型及給藥組大鼠，測定各模型及給藥組大鼠潰瘍指數(shù)及血清中腫瘤壞死因子(TNF-a)、白介素-8(IL-8)、內(nèi)皮素(ET)、一氧化氮(NO)、一氧化氮合酶(INOS)含量；2、胃組織勻漿檢測表皮生長因子(EGF)、轉(zhuǎn)化生長因子(TGF-a)、超氧化物歧化酶(SOD)、丙二醛(MDA)含量；3、腦組織勻漿檢測5-羥色胺(5-HT)、去甲腎上腺素(NE)含量。 結(jié)果：1、在潰瘍指數(shù)方面，寒水組、熱水組與單水組比，明顯升高(P＜0．01)；寒水理、熱水大分別與寒水、熱水組比，明顯降低(P＜0．01)。2、在SOD、EGF及TGF-a方面，寒水、熱水與空白組比較，，均有不同程度的下降(P＜0．01)，熱水組TGF-a含量低于寒水組(P＜0．01)；寒水理與寒水、熱水大與熱水比較，含量均明顯升高(P＜0．01或P＜0．05)。3、在MDA、NO、INOS、IL-8、TNF-a及NE方面，熱水與空白、寒水組比較，含量升高(P＜0．01或P＜0．05)；大黃黃連瀉心湯治療后，熱水大與熱水比較，含量降低(P＜0．05或P＜0．01)。4、在ET、5-HT方面，寒水組與空白、熱水組比較，升高明顯(P＜0．01)；理中丸治療后，寒水理與寒水比較，含量明顯降低(P＜0．01)。 結(jié)論： 1、水浸應(yīng)激型潰瘍寒、熱證的發(fā)病機(jī)制既與整體因素如神經(jīng)-內(nèi)分泌失調(diào)有關(guān)，又與局部因素如胃黏膜保護(hù)功能減弱和損傷因素增強(qiáng)有關(guān)。 2、水浸應(yīng)激型潰瘍熱證與超氧化物歧化酶(SOD)、表皮生長因子(EGF)、轉(zhuǎn)化生長因子(TGF-a)含量降低有關(guān)；與丙二醛(MDA)、一氧化氮(NO)、一氧化氮合酶(INOS)、腫瘤壞死因子(TNF-a)、白介素-8(IL-8)、去甲腎上腺素(NE)含量升高有關(guān)。 3、水浸應(yīng)激型潰瘍寒證與超氧化物歧化酶(SOD)、一氧化氮(NO)、表皮生長因子(EGF)、轉(zhuǎn)化生長因子(TGF-a)含量降低有關(guān)；與內(nèi)皮素(ET)、5-羥色胺(5-HT)含量升高有關(guān)。 4、寒性方劑大黃黃連瀉心湯預(yù)防水浸應(yīng)激型潰瘍熱證的作用機(jī)制可能與提高SOD活性、降低MDA含量、減少NO、INO S的炎性反應(yīng)、刺激胃黏膜EGF、TGF-a釋放、抑制炎性因子IL-8、TNF-a產(chǎn)生、降低交感-腎上腺髓質(zhì)機(jī)能活動而減少NE含量有關(guān)。 5、熱性方劑理中丸預(yù)防水浸應(yīng)激型潰瘍寒證的作用機(jī)制可能與提高SOD活性、提高NO含量、降低ET含量而改善胃黏膜血流量、刺激胃黏膜EGF、TGF-a釋放、提高交感-腎上腺髓質(zhì)機(jī)能活動而降低5-HT含量有關(guān)。
[Abstract]:Objective: to explore the mechanism of stress ulcer cold and heat syndrome and reveal the essence of stress ulcer cold and heat syndrome by combining the water-immersion stress gastric ulcer disease model with traditional Chinese medicine cold and heat syndrome. To observe the action mechanism and the corresponding law of formula of rhubarb, Huanglian Xiexin decoction and Lizhong Pill on stress type ulcer model of cold and heat syndromes, and to observe the effect of rhubarb and Huanglian Xiexin decoction on the model of stress ulcer and heat syndrome. Methods: cold factors (ice water, 0.3mol/L sodium hydroxide) and heat factors (8% capsicum powder, 60% ethanol) were used to establish gastric cold and gastric heat syndrome model. Heat syndrome combined model, cold formula rhubarb, Huanglian Xiexin decoction, heat prescription Lizhong pills were treated by gastric perfusion. 1. After 7 hours of restraint water immersion stress, the rats of each model and drug group were killed. The ulcer index and serum levels of tumor necrosis factor (TNF-a), interleukin-8 (IL-8), endothelin (ET),) nitric oxide (NO), synthase (INOS) were measured in each model and administration group. 2. The contents of epidermal growth factor (EGF), transforming growth factor (TGF-a) and superoxide dismutase (SOD),) malondialdehyde (MDA) were detected in gastric homogenate. 3. The contents of 5-hydroxytryptamine (5-HT) and norepinephrine (NE) were measured in brain homogenate. Results: 1, the ulcer index of cold water group, hot water group and single water group were significantly higher than that of single water group (P < 0.01). Compared with cold water group and hot water group, cold water and hot water decreased significantly (P < 0. 01). 2. In SOD,EGF and TGF-a, cold water and hot water decreased in different degrees compared with blank group (P < 0. 01). The content of TGF-a in hot water group was lower than that in cold water group (P < 0. 01). The contents of cold water, hot water and hot water were significantly higher than those of hot water (P < 0. 01 or P < 0. 05). 3. In MDA,NO,INOS,IL-8,TNF-a and NE, hot water was compared with blank and cold water group. The content increased (P < 0.01 or P < 0.05). After treatment with rhubarb Huanglian Xiexin decoction, the content of hot water was lower than that of hot water (P < 0. 05 or P < 0. 01). 4. In ET,5-HT, compared with blank and hot water group, the content of rhubarb in cold water group was significantly higher than that in hot water group (P < 0. 01). Compared with cold water, the content of cold water decreased significantly (P < 0.01). Conclusion: 1. The pathogenesis of water-immersion stress type ulcers is not only related to the overall factors such as neuroendocrine disorders, but also to local factors such as weakened gastric mucosal protection and increased injury factors. 2, the heat syndrome of water-immersion stress ulcer was related to the decrease of the content of superoxide dismutase (SOD), epidermal growth factor (EGF), transforming growth factor (TGF-a); It was associated with the increase of (INOS), tumor necrosis factor (TNF-a), interleukin-8 (IL-8) and norepinephrine (NE) in malondialdehyde (MDA), nitric oxide (NO),) nitric oxide synthase (INOS),). 3. The cold syndrome of water-immersion stress ulcer was related to the decrease of the content of superoxide dismutase (SOD), nitric oxide (NO), epidermal growth factor (EGF), transforming growth factor (TGF-a). Endothelin 5-hydroxytryptamine (5-HT) is associated with an increase in endothelin 5-hydroxytryptamine (5-HT) content. 4. The mechanism of rhubarb Huanglian Xiexin decoction in preventing heat syndrome of water-immersion stress ulcer may be related to increasing SOD activity, decreasing MDA content, reducing inflammatory reaction of NO,INO S and stimulating EGF,TGF-a release from gastric mucosa. Inhibition of inflammatory factor IL-8,TNF-a and decrease of sympathetic-adrenomedullary activity and decrease of NE content. 5. The mechanism of Lizhong Pill in preventing cold syndrome of water-immersion stress ulcer may be related to increasing SOD activity, increasing NO content, decreasing ET content, improving gastric mucosal blood flow and stimulating gastric mucosal EGF,TGF-a release. Increased sympathetic-adrenomedullary activity and decreased 5-HT content.
【學(xué)位授予單位】：黑龍江中醫(yī)藥大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2009
【分類號】：R-332;R285
本文編號：2346776
[Abstract]:Objective: to explore the mechanism of stress ulcer cold and heat syndrome and reveal the essence of stress ulcer cold and heat syndrome by combining the water-immersion stress gastric ulcer disease model with traditional Chinese medicine cold and heat syndrome. To observe the action mechanism and the corresponding law of formula of rhubarb, Huanglian Xiexin decoction and Lizhong Pill on stress type ulcer model of cold and heat syndromes, and to observe the effect of rhubarb and Huanglian Xiexin decoction on the model of stress ulcer and heat syndrome. Methods: cold factors (ice water, 0.3mol/L sodium hydroxide) and heat factors (8% capsicum powder, 60% ethanol) were used to establish gastric cold and gastric heat syndrome model. Heat syndrome combined model, cold formula rhubarb, Huanglian Xiexin decoction, heat prescription Lizhong pills were treated by gastric perfusion. 1. After 7 hours of restraint water immersion stress, the rats of each model and drug group were killed. The ulcer index and serum levels of tumor necrosis factor (TNF-a), interleukin-8 (IL-8), endothelin (ET),) nitric oxide (NO), synthase (INOS) were measured in each model and administration group. 2. The contents of epidermal growth factor (EGF), transforming growth factor (TGF-a) and superoxide dismutase (SOD),) malondialdehyde (MDA) were detected in gastric homogenate. 3. The contents of 5-hydroxytryptamine (5-HT) and norepinephrine (NE) were measured in brain homogenate. Results: 1, the ulcer index of cold water group, hot water group and single water group were significantly higher than that of single water group (P < 0.01). Compared with cold water group and hot water group, cold water and hot water decreased significantly (P < 0. 01). 2. In SOD,EGF and TGF-a, cold water and hot water decreased in different degrees compared with blank group (P < 0. 01). The content of TGF-a in hot water group was lower than that in cold water group (P < 0. 01). The contents of cold water, hot water and hot water were significantly higher than those of hot water (P < 0. 01 or P < 0. 05). 3. In MDA,NO,INOS,IL-8,TNF-a and NE, hot water was compared with blank and cold water group. The content increased (P < 0.01 or P < 0.05). After treatment with rhubarb Huanglian Xiexin decoction, the content of hot water was lower than that of hot water (P < 0. 05 or P < 0. 01). 4. In ET,5-HT, compared with blank and hot water group, the content of rhubarb in cold water group was significantly higher than that in hot water group (P < 0. 01). Compared with cold water, the content of cold water decreased significantly (P < 0.01). Conclusion: 1. The pathogenesis of water-immersion stress type ulcers is not only related to the overall factors such as neuroendocrine disorders, but also to local factors such as weakened gastric mucosal protection and increased injury factors. 2, the heat syndrome of water-immersion stress ulcer was related to the decrease of the content of superoxide dismutase (SOD), epidermal growth factor (EGF), transforming growth factor (TGF-a); It was associated with the increase of (INOS), tumor necrosis factor (TNF-a), interleukin-8 (IL-8) and norepinephrine (NE) in malondialdehyde (MDA), nitric oxide (NO),) nitric oxide synthase (INOS),). 3. The cold syndrome of water-immersion stress ulcer was related to the decrease of the content of superoxide dismutase (SOD), nitric oxide (NO), epidermal growth factor (EGF), transforming growth factor (TGF-a). Endothelin 5-hydroxytryptamine (5-HT) is associated with an increase in endothelin 5-hydroxytryptamine (5-HT) content. 4. The mechanism of rhubarb Huanglian Xiexin decoction in preventing heat syndrome of water-immersion stress ulcer may be related to increasing SOD activity, decreasing MDA content, reducing inflammatory reaction of NO,INO S and stimulating EGF,TGF-a release from gastric mucosa. Inhibition of inflammatory factor IL-8,TNF-a and decrease of sympathetic-adrenomedullary activity and decrease of NE content. 5. The mechanism of Lizhong Pill in preventing cold syndrome of water-immersion stress ulcer may be related to increasing SOD activity, increasing NO content, decreasing ET content, improving gastric mucosal blood flow and stimulating gastric mucosal EGF,TGF-a release. Increased sympathetic-adrenomedullary activity and decreased 5-HT content.
【學(xué)位授予單位】：黑龍江中醫(yī)藥大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2009
【分類號】：R-332;R285
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