不同的幼年成長環(huán)境對小鼠學(xué)習(xí)記憶的影響
發(fā)布時間：2018-11-05 14:12
【摘要】： 豐富的環(huán)境對人和動物的學(xué)習(xí)記憶能力具有促進(jìn)作用,并可補償腦損傷導(dǎo)致的障礙及神經(jīng)退化,而對中樞神經(jīng)系統(tǒng)發(fā)生影響的生理機(jī)制仍不清楚。本研究探討了豐富和貧乏的環(huán)境對幼年昆明小鼠(Mus musculus Km)的空間性學(xué)習(xí)記憶、情緒性學(xué)習(xí)記憶的影響及其內(nèi)在機(jī)制。 3周齡幼年昆明小鼠被分為以下3組:對照組,豐富環(huán)境組,貧乏環(huán)境組。從環(huán)境處理第4周開始,每周從各組中隨機(jī)選取5只動物,每天腹腔注射5-溴尿嘧啶(5-BrdU)(100mg/Kg)1次,連續(xù)5天,第6天斷頭取材進(jìn)行新生神經(jīng)元的標(biāo)記,并用TUNEL法檢測各組動物凋亡神經(jīng)元的數(shù)量,連續(xù)檢測3周,第7周采用水迷宮及電跳臺方法對小鼠的空間和情緒性學(xué)習(xí)與記憶能力進(jìn)行測試,并測定小鼠的顳皮層及海馬勻漿中的MDA(丙二醛)及SOD(超氧化物歧化酶)含量,以此來研究不同環(huán)境處理對小鼠行為學(xué)改變及機(jī)體內(nèi)氧化應(yīng)激水平變化之間的關(guān)系。 研究結(jié)果發(fā)現(xiàn):(1)貧乏環(huán)境暴露后,小鼠前期活動力下降,后期攻擊力增強,易激怒。而豐富環(huán)境暴露的小鼠則比較溫順。(2)水迷宮測試中,與對照組相比,貧乏環(huán)境組(impoverished growth environment,IGE)找到平臺的潛伏期僅在第3天明顯延長;與豐富環(huán)境組(enriched growth environment,EGE)相比,第2天、第3天IGE組找到平臺的潛伏期均比EGE組明顯延長。電跳臺測試中,與對照組相比,IGE組第1天跳上平臺的潛伏期明顯高于后者;對錯誤次數(shù)的統(tǒng)計結(jié)果表明,第1天與對照組相比,IGE組的錯誤次數(shù)明顯更多,第2天雖然發(fā)生錯誤的次數(shù)有所減少,但仍比對照組多;此外,與EGE組相比,IGE組在發(fā)生錯誤的次數(shù)上明顯高于后者。(3)取10%顳皮層及海馬組織勻漿后測得的SOD值與對照組相比,EGE組明顯升高,IGE組明顯下降;此外,IGE組與EGE組相比SOD值顯著下降。在10%顳皮層及海馬組織勻漿后測得的MDA值,與對照組相比,EGE組明顯下降,IGE組明顯升高;此外,IGE組與EGE組相比MDA值顯著升高。(4)采用5-BrdU標(biāo)記法檢測海馬的CA3區(qū)新生神經(jīng)元,EGE組有較多數(shù)量的陽性細(xì)胞著色:IGE組則基本沒有陽性細(xì)胞著色,大多為空泡;對照組則不明顯。采用TUNEL法檢測凋亡神經(jīng)元,在海馬的CA3區(qū),IGE組有較多數(shù)量的陽性細(xì)胞著色;EGE組則基本沒有;對照組則僅少量或個別陽性細(xì)胞著色。 上述結(jié)果表明,不同的幼年成長環(huán)境對小鼠的空間及情緒性學(xué)習(xí)記憶能力可發(fā)生顯著的影響,其機(jī)制可能與神經(jīng)元氧化應(yīng)激作用和神經(jīng)元的新生、凋亡有關(guān)。
[Abstract]:The rich environment can promote the learning and memory ability of human and animal, and can compensate for the disturbance and nerve degeneration caused by brain injury. However, the physiological mechanism of the effect on the central nervous system is still unclear. The purpose of this study was to investigate the effects of rich and poor environment on spatial learning and memory, emotional learning and memory in young Kunming mice. Three-week old Kunming mice were divided into three groups: control group, rich environment group and poor environment group. From the 4th week of environmental treatment, 5 animals were randomly selected from each group every week. 5 brominouracil (5-BrdU) (100mg/Kg) was injected intraperitoneally once a day for 5 days. The number of apoptotic neurons in each group was detected by TUNEL method, and the ability of spatial and emotional learning and memory in mice was tested by water maze and electric platform method in 7 weeks. The contents of MDA (malondialdehyde) and SOD (superoxide dismutase) in temporal cortex and hippocampal homogenate of mice were measured to study the relationship between the changes of behavior and the level of oxidative stress in mice treated with different environments. The results were as follows: (1) after exposure to poor environment, the activity of mice decreased in Prophase, and increased in later stage. (2) in the water maze test, compared with the control group, the incubation period of the poor environment group (impoverished growth environment,IGE) to find the platform was significantly prolonged on the third day. The latency of finding platform in IGE group was significantly longer than that in EGE group on day 2 and day 3 compared with that in enriched environment group (enriched growth environment,EGE). Compared with the control group, the latency of jumping on the platform in the IGE group was significantly higher than that in the control group on the first day. The results showed that the number of errors in the IGE group was significantly higher than that in the control group on the first day, and the number of errors on the second day was lower, but still more than that in the control group. In addition, the frequency of errors in IGE group was significantly higher than that in EGE group. (3) compared with control group, SOD value of 10% temporal cortex and hippocampal homogenate was significantly increased in EGE group and decreased significantly in IGE group. In addition, the SOD value of IGE group was significantly lower than that of EGE group. Compared with the control group, the MDA values measured after 10% homogenate of temporal cortex and hippocampal tissue were significantly decreased in EGE group and increased in IGE group. In addition, the MDA value of IGE group was significantly higher than that of EGE group. (4) 5-BrdU labeling method was used to detect the new neurons in the CA3 area of hippocampus. In the EGE group, a large number of positive cells were stained. In the IGE group, there were almost no positive cells staining, most of them were vacuoles. The control group was not obvious. TUNEL method was used to detect apoptotic neurons. In the CA3 area of hippocampus, there were a large number of positive cells in IGE group, but no positive cells in EGE group, and only a few or individual positive cells in control group. These results suggest that the spatial and emotional learning and memory abilities of mice can be significantly affected by different juvenile growth environments, and the mechanism may be related to oxidative stress, neuronal regeneration and apoptosis.
【學(xué)位授予單位】：華中師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2008
【分類號】：R33
本文編號：2312323
[Abstract]:The rich environment can promote the learning and memory ability of human and animal, and can compensate for the disturbance and nerve degeneration caused by brain injury. However, the physiological mechanism of the effect on the central nervous system is still unclear. The purpose of this study was to investigate the effects of rich and poor environment on spatial learning and memory, emotional learning and memory in young Kunming mice. Three-week old Kunming mice were divided into three groups: control group, rich environment group and poor environment group. From the 4th week of environmental treatment, 5 animals were randomly selected from each group every week. 5 brominouracil (5-BrdU) (100mg/Kg) was injected intraperitoneally once a day for 5 days. The number of apoptotic neurons in each group was detected by TUNEL method, and the ability of spatial and emotional learning and memory in mice was tested by water maze and electric platform method in 7 weeks. The contents of MDA (malondialdehyde) and SOD (superoxide dismutase) in temporal cortex and hippocampal homogenate of mice were measured to study the relationship between the changes of behavior and the level of oxidative stress in mice treated with different environments. The results were as follows: (1) after exposure to poor environment, the activity of mice decreased in Prophase, and increased in later stage. (2) in the water maze test, compared with the control group, the incubation period of the poor environment group (impoverished growth environment,IGE) to find the platform was significantly prolonged on the third day. The latency of finding platform in IGE group was significantly longer than that in EGE group on day 2 and day 3 compared with that in enriched environment group (enriched growth environment,EGE). Compared with the control group, the latency of jumping on the platform in the IGE group was significantly higher than that in the control group on the first day. The results showed that the number of errors in the IGE group was significantly higher than that in the control group on the first day, and the number of errors on the second day was lower, but still more than that in the control group. In addition, the frequency of errors in IGE group was significantly higher than that in EGE group. (3) compared with control group, SOD value of 10% temporal cortex and hippocampal homogenate was significantly increased in EGE group and decreased significantly in IGE group. In addition, the SOD value of IGE group was significantly lower than that of EGE group. Compared with the control group, the MDA values measured after 10% homogenate of temporal cortex and hippocampal tissue were significantly decreased in EGE group and increased in IGE group. In addition, the MDA value of IGE group was significantly higher than that of EGE group. (4) 5-BrdU labeling method was used to detect the new neurons in the CA3 area of hippocampus. In the EGE group, a large number of positive cells were stained. In the IGE group, there were almost no positive cells staining, most of them were vacuoles. The control group was not obvious. TUNEL method was used to detect apoptotic neurons. In the CA3 area of hippocampus, there were a large number of positive cells in IGE group, but no positive cells in EGE group, and only a few or individual positive cells in control group. These results suggest that the spatial and emotional learning and memory abilities of mice can be significantly affected by different juvenile growth environments, and the mechanism may be related to oxidative stress, neuronal regeneration and apoptosis.
【學(xué)位授予單位】：華中師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2008
【分類號】：R33
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