【摘要】：目的探討上海市青浦區(qū)流行的結(jié)核分枝桿菌(MTB)的主要基因型及其與耐藥表型的關(guān)系。方法分別提取266株MTB DNA,應(yīng)用多重聚合酶鏈反應(yīng)(PCR)對(duì)其進(jìn)行分型(北京基因型/非北京基因型)鑒定;采用比例法對(duì)此266株菌株進(jìn)行利福平、異煙肼、鏈霉素和乙胺丁醇的藥物敏感性試驗(yàn);運(yùn)用SPSS 17.0統(tǒng)計(jì)軟件分析基因分型與患者性別、年齡的關(guān)系,以及基因分型與菌株耐藥性之間的關(guān)系。結(jié)果 266株菌株中北京基因型242株(90.98%),非北京基因型24株(9.02%)。北京基因型菌株的總耐藥率為23.96%,耐多藥率為9.91%,非北京基因型的總耐藥率和耐多藥率分別為29.16%和8.33%,兩者差異無統(tǒng)計(jì)學(xué)意義(P均0.05)。結(jié)論北京基因型MTB可能是上海市青浦區(qū)的主要流行株,尚未發(fā)現(xiàn)北京基因型菌株和非北京基因型菌株在耐藥方面有明顯差異。
[Abstract]:Objective to investigate the main genotypes of Mycobacterium tuberculosis (MTB) and its relationship with drug resistance phenotype in Qingpu District, Shanghai. Methods 266 MTB DNA, strains were identified by multiplex polymerase chain reaction (PCR), and rifampicin and isoniazid were detected by proportional method. The drug sensitivity test of streptomycin and ethambutanol, the relationship between genotyping and patient sex, age, and the relationship between genotyping and drug resistance were analyzed by SPSS 17.0 software. Results among 266 strains, 242 (90.98%) were Beijing genotypes and 24 (9.02%) were non-Beijing genotypes. The total drug resistance rate of Beijing genotype was 23.96, the multi-drug resistance rate was 9.91, the total drug resistance rate and multi-drug resistance rate of non-Beijing genotype were 29.16% and 8.33%, respectively. There was no significant difference between them (P 0.05). Conclusion Beijing genotype MTB may be the main epidemic strain in Qingpu district of Shanghai, but there is no obvious difference between Beijing genotype strain and non-Beijing genotype strain in drug resistance.
【作者單位】： 上海市青浦區(qū)中心醫(yī)院檢驗(yàn)科;
【基金】：上海市青浦區(qū)中心醫(yī)院院級(jí)課題(QY2012-12)
【分類號(hào)】：R378.911

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