韁核在視交叉上核—松果體晝夜節(jié)律控制系統(tǒng)中的作用研究
發(fā)布時間：2018-09-04 05:58
【摘要】： 晝夜節(jié)律是生物的基本生命特征之一,它的穩(wěn)定是機(jī)體正常生理功能進(jìn)行的基礎(chǔ)。許多資料證明:外界光照可通過下丘腦視交叉上核(SCN)和松果體(PG)來調(diào)節(jié)晝夜節(jié)律,并使內(nèi)源性晝夜節(jié)律和環(huán)境的24小時晝夜節(jié)律同步,提示SCN和PG在機(jī)體晝夜節(jié)律的調(diào)節(jié)中起重要作用,韁核(Hb)密切聯(lián)系這兩個結(jié)構(gòu),并且Hb本身也有視網(wǎng)膜纖維投射,因此,Hb可能介導(dǎo)SCN—PG晝夜節(jié)律的信息傳遞,與SCN和PG等結(jié)構(gòu)共同組成中樞晝夜節(jié)律控制系統(tǒng)。 本實(shí)驗(yàn)首先應(yīng)用電生理學(xué)方法,觀察電刺激或化學(xué)刺激興奮SCN及化學(xué)阻斷SCN,對外側(cè)韁核(LHb)和內(nèi)側(cè)韁核(MHb)神經(jīng)元自發(fā)放電的影響,從細(xì)胞水平弄清SCN與Hb間的功能聯(lián)系。進(jìn)一步應(yīng)用逆轉(zhuǎn)錄聚合酶鏈法(RT-PCR),在海人酸(KA)損毀LHb2周后,檢測SCN和PG在六個不同時間點(diǎn)的時鐘基因Per2、Bmal1和Clock mRNA的表達(dá)水平,以及褪黑素(MT)合成限速酶N-乙酰轉(zhuǎn)移酶(NAT)及其受體MT1和MT2 mRNA的表達(dá)水平。探討SCN和PG的晝夜節(jié)律相關(guān)基因和物質(zhì)的表達(dá)是否需要有Hb的存在,從另一角度明確Hb的作用。 實(shí)驗(yàn)結(jié)果顯示:50%Hb神經(jīng)元對SCN刺激有反應(yīng),且不同部位的Hb神經(jīng)元對SCN刺激的電生理反應(yīng)不同,即MHb主要表現(xiàn)為抑制,而LHb則沒有明顯趨勢,但SCN被阻斷后對LHb起抑制效應(yīng);LHb損毀后PG和SCN內(nèi)NAT以及MT1、MT2 mRNA在不同時間點(diǎn)的表達(dá)多下調(diào),但其表達(dá)的周期沒有改變;SCN和PG中時鐘基因Per2、Bmal1和Clock的晝夜表達(dá)水平,在損毀組也多顯著下降,但其周期也無變化。以上結(jié)果提示LHb與SCN和PG之間具有密切的功能聯(lián)系,LHb可通過影響PG活動進(jìn)而影響SCN的功能,同時LHb也接受SCN和PG的調(diào)控。LHb可能通過影響PG內(nèi)褪黑素的合成及其受體的表達(dá),進(jìn)而影響時鐘基因的表達(dá),參與中樞晝夜節(jié)律調(diào)節(jié),成為中樞晝夜節(jié)律調(diào)控系統(tǒng)組構(gòu)中的重要成分。
[Abstract]:Circadian rhythm is one of the basic biological characteristics, and its stability is the basis of normal physiological function. Many data have shown that the circadian rhythm can be regulated by (SCN) and (PG) in the supraoptic nucleus of hypothalamus, and the circadian rhythm of the endogenous circadian rhythm is synchronized with the circadian rhythm of the environment in 24 hours. It is suggested that SCN and PG play an important role in the regulation of circadian rhythm. Habenula (Hb) is closely related to these two structures, and Hb itself also has retinal fiber projection. Therefore, Hb may mediate the transmission of SCN-PG circadian rhythm. The central circadian rhythm control system is composed of SCN and PG. In this study, we first observed the effects of electrical or chemical stimulation on the spontaneous discharges of (LHb) and (MHb) neurons in the lateral habenular nucleus and the medial habenular nucleus by electrical or chemical stimulation of excited SCN and chemically blocked SCN, and clarified the functional relationship between SCN and Hb at the cellular level. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect the expression of clock genes Per2,Bmal1 and Clock mRNA in SCN and PG at six different time points after kainic acid (KA) destroyed LHb2 week. And the expression level of melatonin (MT) synthesis rate-limiting enzyme N-acetyltransferase (NAT) and its receptors MT1 and MT2 mRNA. To investigate whether the presence of Hb is necessary for the expression of circadian rhythm related genes and substances of SCN and PG, and to clarify the role of Hb from another perspective. The results showed that the HB neurons in the ratio of 50 to 50 had a response to SCN stimulation, and the electrophysiological responses of Hb neurons in different parts to SCN stimulation were different. That is, MHb was mainly inhibited, but LHb had no obvious tendency. The expression of NAT and MT1,MT2 mRNA in PG and SCN were down-regulated at different time points, but the expression cycle of NAT and Clock in PG and PG did not change the circadian expression level of clock genes Per2,Bmal1 and Clock in PG and PG. It also decreased significantly in the lesion group, but its cycle did not change. These results suggest that there is a close functional relationship between LHb and SCN and PG. LHb may affect the function of SCN by affecting the activity of PG, and LHb also accepts the regulation of SCN and PG. LHb may affect the synthesis of melatonin and the expression of its receptors in PG. Furthermore, the expression of clock gene is affected, and it participates in the regulation of central circadian rhythm and becomes an important component in the system of central circadian rhythm regulation.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2009
【分類號】：R33
本文編號：2221219
[Abstract]:Circadian rhythm is one of the basic biological characteristics, and its stability is the basis of normal physiological function. Many data have shown that the circadian rhythm can be regulated by (SCN) and (PG) in the supraoptic nucleus of hypothalamus, and the circadian rhythm of the endogenous circadian rhythm is synchronized with the circadian rhythm of the environment in 24 hours. It is suggested that SCN and PG play an important role in the regulation of circadian rhythm. Habenula (Hb) is closely related to these two structures, and Hb itself also has retinal fiber projection. Therefore, Hb may mediate the transmission of SCN-PG circadian rhythm. The central circadian rhythm control system is composed of SCN and PG. In this study, we first observed the effects of electrical or chemical stimulation on the spontaneous discharges of (LHb) and (MHb) neurons in the lateral habenular nucleus and the medial habenular nucleus by electrical or chemical stimulation of excited SCN and chemically blocked SCN, and clarified the functional relationship between SCN and Hb at the cellular level. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect the expression of clock genes Per2,Bmal1 and Clock mRNA in SCN and PG at six different time points after kainic acid (KA) destroyed LHb2 week. And the expression level of melatonin (MT) synthesis rate-limiting enzyme N-acetyltransferase (NAT) and its receptors MT1 and MT2 mRNA. To investigate whether the presence of Hb is necessary for the expression of circadian rhythm related genes and substances of SCN and PG, and to clarify the role of Hb from another perspective. The results showed that the HB neurons in the ratio of 50 to 50 had a response to SCN stimulation, and the electrophysiological responses of Hb neurons in different parts to SCN stimulation were different. That is, MHb was mainly inhibited, but LHb had no obvious tendency. The expression of NAT and MT1,MT2 mRNA in PG and SCN were down-regulated at different time points, but the expression cycle of NAT and Clock in PG and PG did not change the circadian expression level of clock genes Per2,Bmal1 and Clock in PG and PG. It also decreased significantly in the lesion group, but its cycle did not change. These results suggest that there is a close functional relationship between LHb and SCN and PG. LHb may affect the function of SCN by affecting the activity of PG, and LHb also accepts the regulation of SCN and PG. LHb may affect the synthesis of melatonin and the expression of its receptors in PG. Furthermore, the expression of clock gene is affected, and it participates in the regulation of central circadian rhythm and becomes an important component in the system of central circadian rhythm regulation.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2009
【分類號】：R33
【引證文獻(xiàn)】
相關(guān)會議論文 前1條
1 曹靜;李萌;鐘奇;沈應(yīng)博;殷立偉;錢婉盈;楊夏云;陳耀星;;組胺在雞視網(wǎng)膜節(jié)細(xì)胞層的分布[A];中國畜牧獸醫(yī)學(xué)會動物解剖學(xué)及組織胚胎學(xué)分會第十七次學(xué)術(shù)研討會論文集（下）[C];2012年
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