【摘要】： 開發(fā)可培養(yǎng)的嗜鹽微生物并檢測它們潛在的抗菌和抗腫瘤活性,從中發(fā)現(xiàn)新穎的化合物,將具有重要的理論意義和應用價值。 對從山東省威海鹽場采集的底泥和海水樣品中存在的微生物進行了分離。測定了各種嗜鹽菌株的生長特性,并依據(jù)其嗜鹽特性進行了分類。通過形態(tài)觀察和分子生物學方法對嗜鹽菌株進行了分類鑒定和系統(tǒng)發(fā)育分析。對嗜鹽菌株進行抑菌活性篩選,并測定嗜鹽菌株的粗提取物對腫瘤細胞生長的抑制作用。 從鹽場樣品中共分離到45株細菌、28株真菌和38株放線菌,都具有中度嗜鹽特性。嗜鹽細菌的系統(tǒng)發(fā)育分析表明,17個菌株屬于厚壁菌門(Firmicutes),分屬于芽孢桿菌屬(Bacillus)、喜鹽芽孢桿菌屬(Halobacillus)、動性球菌屬(Planococcus)和鹽水球菌屬(Salinicoccus);其他28個菌株均屬于γ-變形細菌綱(γ-Proteobacteria)的鹽單胞菌屬(Halomonas)。嗜鹽真菌均屬于真菌門(Eumycota)的半知菌亞門(Deuteromycotina),9個菌株屬于曲霉屬(Aspergillus),19個菌株屬于青霉屬(Penicillium)。嗜鹽放線菌屬于放線菌門(Actinobacteria),主要以鏈霉菌屬(Streptomyces)為主。 嗜鹽細菌對革蘭氏陽性菌、多種人病原真菌和植物病原真菌都表現(xiàn)出較強的抑制作用;嗜鹽真菌對革蘭氏陽性和陰性細菌都有明顯的抑制作用。14株細菌、13株真菌和20株放線菌的粗提物對腫瘤細胞都表現(xiàn)出很強的抑制作用。 結(jié)果表明,嗜鹽微生物可作為發(fā)現(xiàn)新穎生物活性物質(zhì)的潛在資源。
[Abstract]:The development of culturable halophilic microorganisms and the detection of their potential antimicrobial and antitumor activities, and the discovery of novel compounds will be of great theoretical significance and application value. Microbes were isolated from sediment and seawater samples collected from Weihai Salt Farm, Shandong Province. The growth characteristics of various halophilic strains were determined and classified according to their halophilic characteristics. The halophilic strains were identified and phylogenetic analysis by morphological observation and molecular biology. The antimicrobial activity of halophilic strain was screened, and the inhibitory effect of crude extract of halophilic strain on tumor cell growth was determined. A total of 45 strains of bacteria, 28 strains of fungi and 38 strains of actinomycetes were isolated from salt field samples. Phylogenetic analysis of halophilic bacteria shows that 17 strains belong to the phylum (Firmicutes), of Bacillus (Bacillus), Bacillus, (Bacillus), Bacillus, (Halobacillus), (Planococcus), and the other 28 strains of Salina, (Salinicoccus);, all belong to (Halomonas)., which belongs to 緯 -Proteobacteria. Halophilic fungi belong to (Eumycota) subphylum (Deuteromycotina), 9 strains belong to Aspergillus (Aspergillus), 19 strains belong to Penicillium (Penicillium). Halophilic actinomycetes belong to (Actinobacteria), of actinomycetes, mainly Streptomyces (Streptomyces). Halophilic bacteria showed strong inhibition on Gram-positive bacteria, various human pathogenic fungi and plant pathogenic fungi. The crude extracts of 14 strains of bacteria, 13 strains of fungi and 20 strains of actinomycetes showed strong inhibitory effects on tumor cells. The results showed that halophilic microorganisms could be used as potential resources for the discovery of novel bioactive substances.
【學位授予單位】：中國科學院研究生院（海洋研究所）
【學位級別】：博士
【學位授予年份】：2009
【分類號】：R371

【參考文獻】

相關期刊論文 前5條

1 劉愛民;嗜鹽菌的研究進展[J];安徽師范大學學報(自然科學版);2002年02期

2 汪天虹,侯運華;海洋極端微生物和極端酶分子生物學研究[J];科技導報;2004年09期

3 唐蜀昆;姜怡;職曉陽;婁愷;李文均;徐麗華;;嗜鹽放線菌分離方法[J];微生物學通報;2007年02期

4 董錫文,薛春梅,吳玉德;極端微生物及其適應機理的研究進展[J];微生物學雜志;2005年01期

5 張永光,李文均,姜成林,張忠澤;嗜鹽放線菌的研究進展[J];微生物學雜志;2002年04期

，

本文編號：2218300