【摘要】： 開發(fā)可培養(yǎng)的嗜鹽微生物并檢測(cè)它們潛在的抗菌和抗腫瘤活性,從中發(fā)現(xiàn)新穎的化合物,將具有重要的理論意義和應(yīng)用價(jià)值。 對(duì)從山東省威海鹽場(chǎng)采集的底泥和海水樣品中存在的微生物進(jìn)行了分離。測(cè)定了各種嗜鹽菌株的生長特性,并依據(jù)其嗜鹽特性進(jìn)行了分類。通過形態(tài)觀察和分子生物學(xué)方法對(duì)嗜鹽菌株進(jìn)行了分類鑒定和系統(tǒng)發(fā)育分析。對(duì)嗜鹽菌株進(jìn)行抑菌活性篩選,并測(cè)定嗜鹽菌株的粗提取物對(duì)腫瘤細(xì)胞生長的抑制作用。 從鹽場(chǎng)樣品中共分離到45株細(xì)菌、28株真菌和38株放線菌,都具有中度嗜鹽特性。嗜鹽細(xì)菌的系統(tǒng)發(fā)育分析表明,17個(gè)菌株屬于厚壁菌門(Firmicutes),分屬于芽孢桿菌屬(Bacillus)、喜鹽芽孢桿菌屬(Halobacillus)、動(dòng)性球菌屬(Planococcus)和鹽水球菌屬(Salinicoccus);其他28個(gè)菌株均屬于γ-變形細(xì)菌綱(γ-Proteobacteria)的鹽單胞菌屬(Halomonas)。嗜鹽真菌均屬于真菌門(Eumycota)的半知菌亞門(Deuteromycotina),9個(gè)菌株屬于曲霉屬(Aspergillus),19個(gè)菌株屬于青霉屬(Penicillium)。嗜鹽放線菌屬于放線菌門(Actinobacteria),主要以鏈霉菌屬(Streptomyces)為主。 嗜鹽細(xì)菌對(duì)革蘭氏陽性菌、多種人病原真菌和植物病原真菌都表現(xiàn)出較強(qiáng)的抑制作用;嗜鹽真菌對(duì)革蘭氏陽性和陰性細(xì)菌都有明顯的抑制作用。14株細(xì)菌、13株真菌和20株放線菌的粗提物對(duì)腫瘤細(xì)胞都表現(xiàn)出很強(qiáng)的抑制作用。 結(jié)果表明,嗜鹽微生物可作為發(fā)現(xiàn)新穎生物活性物質(zhì)的潛在資源。
[Abstract]:The development of culturable halophilic microorganisms and the detection of their potential antimicrobial and antitumor activities, and the discovery of novel compounds will be of great theoretical significance and application value. Microbes were isolated from sediment and seawater samples collected from Weihai Salt Farm, Shandong Province. The growth characteristics of various halophilic strains were determined and classified according to their halophilic characteristics. The halophilic strains were identified and phylogenetic analysis by morphological observation and molecular biology. The antimicrobial activity of halophilic strain was screened, and the inhibitory effect of crude extract of halophilic strain on tumor cell growth was determined. A total of 45 strains of bacteria, 28 strains of fungi and 38 strains of actinomycetes were isolated from salt field samples. Phylogenetic analysis of halophilic bacteria shows that 17 strains belong to the phylum (Firmicutes), of Bacillus (Bacillus), Bacillus, (Bacillus), Bacillus, (Halobacillus), (Planococcus), and the other 28 strains of Salina, (Salinicoccus);, all belong to (Halomonas)., which belongs to 緯 -Proteobacteria. Halophilic fungi belong to (Eumycota) subphylum (Deuteromycotina), 9 strains belong to Aspergillus (Aspergillus), 19 strains belong to Penicillium (Penicillium). Halophilic actinomycetes belong to (Actinobacteria), of actinomycetes, mainly Streptomyces (Streptomyces). Halophilic bacteria showed strong inhibition on Gram-positive bacteria, various human pathogenic fungi and plant pathogenic fungi. The crude extracts of 14 strains of bacteria, 13 strains of fungi and 20 strains of actinomycetes showed strong inhibitory effects on tumor cells. The results showed that halophilic microorganisms could be used as potential resources for the discovery of novel bioactive substances.
【學(xué)位授予單位】：中國科學(xué)院研究生院（海洋研究所）
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2009
【分類號(hào)】：R371

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本文編號(hào)：2218300