本文選題：獻(xiàn)血者 + HIV感染��； 參考：《山東大學(xué)》2008年碩士論文

【摘要】： 第一部分濟(jì)南地區(qū)無(wú)償獻(xiàn)血者中血源性傳播病毒感染的流行病學(xué)調(diào)查 目的:檢測(cè)濟(jì)南地區(qū)無(wú)償獻(xiàn)血者血漿中乙型肝炎病毒表面抗原(HBsAg)、抗丙型肝炎病毒(HCV)抗體、抗人類免疫缺陷病毒(HIV)抗體以及抗人皰疹病毒8型(HHV-8)IgG抗體,分析我國(guó)濟(jì)南地區(qū)獻(xiàn)血人群中相關(guān)病毒的感染情況和流行病學(xué)特點(diǎn);為濟(jì)南地區(qū)無(wú)償獻(xiàn)血人群的選擇和制定招募安全血源的策略提供理論依據(jù)。 方法: 1.血清學(xué)檢測(cè) 1.1利用ELISA方法對(duì)2006年1月～2006年12月無(wú)償獻(xiàn)血者的血漿標(biāo)本(43904份)進(jìn)行HBsAg、抗-HCV、抗-HIV的檢測(cè);抗-HIV檢測(cè)呈陽(yáng)性者送山東省疾病預(yù)防控制中心進(jìn)行Western-Blot確認(rèn)。 1.2利用ELISA方法對(duì)其中520份獻(xiàn)血者血漿進(jìn)行抗-HHV-8 IgG抗體的檢測(cè),包被抗原為HHV-8結(jié)構(gòu)蛋白ORF K8.1合成多肽。 2.統(tǒng)計(jì)學(xué)處理 2006年1月～2006年12月的獻(xiàn)血者基本資料和各項(xiàng)試驗(yàn)檢測(cè)的結(jié)果應(yīng)用SPSS10.0統(tǒng)計(jì)軟件包進(jìn)行統(tǒng)計(jì)學(xué)分析。統(tǒng)計(jì)學(xué)方法采用x~2檢驗(yàn)。 結(jié)果: 1.43904份獻(xiàn)血者血漿中HBsAg檢出率為2.04%(898/43904),抗-HCV檢出率為0.78%(344/43904),抗-HIV檢出率為0.0045%(2/43904)。520份獻(xiàn)血者血漿中抗-HHV-8檢出率為5.962%(31/520)。 2.統(tǒng)計(jì)學(xué)分析結(jié)果顯示,HBsAg、抗-HCV檢出率在不同年齡組間的差異無(wú)統(tǒng)計(jì)學(xué)意義(P＞0.05),在不同職業(yè)間的差異有統(tǒng)計(jì)學(xué)意義(P＜0.05);男性獻(xiàn)血者中HBsAg的檢出率高于女性,差異有統(tǒng)計(jì)學(xué)意義(P＜0.05);不同性別、年齡獻(xiàn)血者血漿中抗-HHV-8的檢出率差異無(wú)統(tǒng)計(jì)學(xué)意義(P＞0.05)。 結(jié)論:濟(jì)南市無(wú)償獻(xiàn)血者中存在一定比率的HCV感染者及相對(duì)較高的HBV、HHV-8感染者,同時(shí)尚存在個(gè)別HIV感染者。在以后的血液篩查工作中有必要進(jìn)一步提高檢測(cè)方法的準(zhǔn)確度以及加強(qiáng)招募前的篩查工作,以不斷提高輸血安全。 第二部分人皰疹病毒8型ORF73C基因原核表達(dá)載體的構(gòu)建 目的: 構(gòu)建人皰疹病毒8型ORF73C基因原核表達(dá)載體,為下一步的基因表達(dá)及重組蛋白診斷試劑盒的制備奠定基礎(chǔ)。 方法: 根據(jù)HHV-8 ORF73C基因序列和相關(guān)文獻(xiàn)設(shè)計(jì)PCR引物,在引物的5'端分別引入限制酶Bam HI和HindⅢ酶切位點(diǎn),以含有HHV-8 ORF73基因片段的重組質(zhì)粒pcDNAHis.LANA1為模板,利用PCR方法擴(kuò)增HHV-8 ORF73C基因,克隆至pGEM T-easy載體,并進(jìn)一步克隆至原核表達(dá)載體pET30(a+)中,構(gòu)建原核表達(dá)質(zhì)粒pETORF73C。 結(jié)果: 1.瓊脂糖凝膠電泳證實(shí)PCR擴(kuò)增產(chǎn)物為目的基因(HHV-8 ORF73C)。 2.DNA測(cè)序證實(shí)HHV-8 ORF73C原核表達(dá)載體pETORF73C構(gòu)建成功。 結(jié)論本研究成功構(gòu)建了含有HHV-8 ORF73C抗原表位的原核表達(dá)質(zhì)粒pETORF73C,為以后的重組蛋白制備奠定了基礎(chǔ)。
[Abstract]:Part I Epidemiological investigation of blood-borne sexually transmitted virus infection among unpaid blood donors in Jinan Objective: to detect hepatitis B virus surface antigen (HBsAg), anti-hepatitis C virus (HCV) antibody, anti-human immunodeficiency virus (HIV) antibody and anti-human herpesvirus 8 (HHV-8) IgG in plasma of blood donors in Jinan area. To analyze the infection and epidemiological characteristics of related viruses in blood donors in Jinan, and to provide a theoretical basis for the selection of free blood donors in Jinan and the formulation of strategies for recruiting safe blood sources. Methods: 1. Serological detection 1.1 A total of 43904 plasma samples from unpaid blood donors from January 2006 to December 2006 were tested for HBsAg, anti-HCV and anti-HIV by ELISA method, and those who were positive for anti-HIV were sent to Shandong Center for Disease Prevention and Control for Western-Blot confirmation. 1.2 the anti-HHV-8 IgG antibody was detected in 520 blood donor plasma by ELISA method. The antigen was HHV-8 structural protein ORF K8.1 synthetic polypeptide. 2. Statistical processing From January 2006 to December 2006, the basic data of blood donors and the results of various tests were statistically analyzed by SPSS10.0 statistical software package. The statistical method was x2 test. Results: The detection rate of HBsAg in plasma of 1.43904 blood donors was 2.04. The positive rate of anti-HCV was 0.78. The detection rate of anti-HCV was 344 / 43904. The detection rate of anti-HIV was 0.0045%. The detection rate of anti-HHV-8 was 5.962%. 2. The results of statistical analysis showed that there was no significant difference in detection rate of HBsAg and anti-HCV among different age groups (P > 0.05), but there was significant difference among different occupations (P < 0.05), and the detection rate of HBsAg in male blood donors was higher than that in women. The difference was statistically significant (P < 0.05), but there was no significant difference in the detection rate of anti-HHV-8 in blood donors of different sex and age (P > 0.05). Conclusion: there are a certain percentage of HCV infection and a relatively high rate of HBV HHV-8 infection among unpaid blood donors in Jinan City, and there are still individual HIV infected individuals. In order to improve the safety of blood transfusion, it is necessary to further improve the accuracy of detection methods and the screening work before recruitment in the future blood screening work. Construction of prokaryotic expression vector of human herpesvirus type 8 ORF73C gene Objective: The prokaryotic expression vector of human herpesvirus type 8 ORF73C gene was constructed, which laid a foundation for the next step of gene expression and preparation of recombinant protein diagnostic kit. Methods: According to the sequence of HHV-8 ORF73C gene and related literatures, PCR primers were designed. Restriction enzyme Bam HI and Hind 鈪，

本文編號(hào)：1927564