發(fā)布時間：2018-05-24 05:08

  本文選題：腦缺血再灌注 + 大腦皮質(zhì)�。� 參考：《南昌大學(xué)》2008年碩士論文

【摘要】： 為了研究腦缺血再灌流后大鼠大腦皮質(zhì)微血管的變化。本實驗采用42只SD大鼠,分為對照組、腦缺血15min再灌流6h、12h、24h組與腦缺血2h再灌流6h、12h、24h組。用結(jié)扎左頸總動脈,夾閉右頸總動脈,再恢復(fù)右頸總動脈血流的方法建立大鼠腦缺血再灌流模型。以堿性磷酸酶法顯示微血管并應(yīng)用體視學(xué)方法測算大鼠海馬、齒狀回及頂葉皮質(zhì)微血管長度密度(Lv)、表面積密度(Sv)、體積密度(Vv)的變化。結(jié)果如下: 1、各組大鼠海馬各區(qū)、齒狀回及頂葉皮質(zhì)區(qū)微血管通過堿性磷酸酶法顯示呈點狀、線狀或短樹枝狀。 2、腦缺血15min再灌流后6h、12h和24h組同對照組比較:大鼠大腦皮質(zhì)微血管的Lv、Sv、Vv隨著存活時間的不同而發(fā)生顯著變化,尤其在再灌流后6h、12h。與對照組相比,Sv在6h、12h、24h減小(P＜0.01),Lv在6h極顯著下降(P＜0.01),12h極顯著上升(P＜0.01)。Vv在6h極顯著上升(P＜0.01),12h顯著上升(P＜0.05)。24h組Lv、Vv同對照組相比無顯著性變化(P＞0.05)。與6h相比,12h組齒狀回及頂葉皮質(zhì)區(qū)微血管Vv具極顯著性差異(P＜0.01),24h組大腦皮質(zhì)微血管Vv具極顯著性差異(P＜0.01)。 3、腦缺血2h再灌流后6h、12h、24h組同對照組比較:6h至24h大鼠大腦皮質(zhì)微血管Lv、Sv顯著低于對照組(P＜0.01)。Vv在6h極顯著上升(P＜0.01),至24h持續(xù)高于對照組(P＜0.01)。 4、腦缺血15min與腦缺血2h在同一時相點比較:6h、12h、24h大鼠大腦海馬、齒狀回及頂葉皮質(zhì)微血管Lv、Sv、Vv均有顯著性差異(P＜0.01)。 5、各組中大鼠左右兩側(cè)同區(qū)比較微血管密度均不存在顯著性差異(P＞0.05)。各組中頂葉皮質(zhì)區(qū)微血管密度高于海馬各區(qū)及齒狀回(P＜0.01)。
[Abstract]:To study the changes of cerebral cortex microvessels after cerebral ischemia reperfusion in rats. 42 Sprague-Dawley rats were divided into two groups: the control group, the brain ischemia 15min reperfusion group (6h) and the cerebral ischemia group (12h), the control group (6h) and the control group (12h). The rat model of cerebral ischemia reperfusion was established by ligating the left common carotid artery, clipping the right common carotid artery and restoring the blood flow of the right common carotid artery. The changes of microvascular length density, surface area density and volume density in hippocampus, dentate gyrus and parietal cortex of rats were measured by alkaline phosphatase method. The results are as follows: 1. The microvessels in hippocampus, dentate gyrus and parietal cortex of each group were shown to be punctate, linear or short dendritic by alkaline phosphatase method. 2. Compared with the control group at 12 h and 24 h after 15min reperfusion, the VV v of rat cortical microvessels changed significantly with the survival time, especially at 6 h and 12 h after reperfusion. Compared with the control group, Sv decreased significantly at 6h and 12h and decreased significantly (P < 0.01) at 6h. There was no significant difference in LvVV between the control group and the control group (P < 0.01). There was no significant difference in the LvVV between the two groups (P < 0.01) and the control group (P > 0.05). There was significant difference in VV between 12 h group and 12 h group compared with 6 h group (P < 0.01). There was significant difference in VV value between 12 h group and 24 h group (P < 0.01). 3. Compared with the control group, the Sv of the cerebral cortex microvessel in the control group was significantly lower than that in the control group (P < 0.01) at 6 h to 24 h after cerebral ischemia and reperfusion (P < 0.01), and remained higher than that in the control group (P < 0.01) at 24 h. 4. There was a significant difference between 15min and VV in hippocampus, dentate gyrus and parietal cortex of rats at the same time (P < 0.01). 5. There was no significant difference in microvessel density (P > 0.05). The microvessel density in middle parietal cortex was higher than that in hippocampus and dentate gyrus (P < 0.01).
【學(xué)位授予單位】：南昌大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2008
【分類號】：R361.2

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 黎海濤,應(yīng)大君,何小川,孫建森;犬燒傷早期腦水腫時毛細(xì)血管密度的體視學(xué)研究[J];第三軍醫(yī)大學(xué)學(xué)報;2001年02期

2 應(yīng)希,陰正勤,王一,孫榆;體視學(xué)技術(shù)在視乳頭血管的定量分析[J];第三軍醫(yī)大學(xué)學(xué)報;2004年02期

3 唐震宇,陸雪芬,易詠紅;阿司匹林對大鼠腦缺血再灌注損傷的影響[J];廣州醫(yī)學(xué)院學(xué)報;2002年03期

4 張其梅,李承晏;大腦中動脈阻斷大鼠軟腦膜微血管的早期變化[J];河南實用神經(jīng)疾病雜志;2002年06期

5 李煒如,姚裕家,譚建三,汪清;新生豬缺氧缺血性腦損傷時的腦血流量改變[J];華西醫(yī)科大學(xué)學(xué)報;1998年01期

6 狄政莉,張琳,萬琪,王洪典,馬鐵柱;大鼠全腦缺血再灌注腦微血管損傷與修復(fù)動態(tài)變化的實驗研究[J];神經(jīng)疾病與精神衛(wèi)生;2001年04期

7 劉文瑞,方秀斌;人海馬區(qū)血管顯微解剖[J];解剖學(xué)雜志;2003年04期

8 楊允東,關(guān)寧,羅俊生;大鼠腦缺血模型血腦屏障結(jié)構(gòu)和功能狀態(tài)的研究[J];錦州醫(yī)學(xué)院學(xué)報;2005年05期

9 馮華松,張勇,汪海;ATP敏感性鉀通道開放劑治療腦缺血的前景[J];中華老年心腦血管病雜志;2001年04期

10 皇甫秋霞,王春海,殷鏡海,王世軍;大腦中動脈阻斷對大鼠軟腦膜微血管管徑及血流速度的影響[J];生物醫(yī)學(xué)工程研究;2005年01期

，

本文編號：1927796