本文選題：小鼠胚胎 + AGM時期��； 參考：《華中科技大學(xué)》2009年博士論文

【摘要】： 第一部分 [目的]探討AGM時期(E10～E12)小鼠造血部位的解剖結(jié)構(gòu)。 [方法]將雌、雄小鼠以3: 1合籠喂養(yǎng),觀察小鼠陰道栓獲得準(zhǔn)確孕期的小鼠胚胎,收集E10, E10.5, E11, E11.5和E12小鼠胚胎,去掉胎膜和胎盤,用4%甲醛固定3h后,進(jìn)行乙醇脫水,石蠟包埋,通過胚胎正中矢狀面組織切片,進(jìn)行HE染色,在顯微鏡下連續(xù)觀察造血部位的組織結(jié)構(gòu)。 [結(jié)果]胚胎正中矢狀面切片,自E10到E12,在腹部背側(cè)并沒有見到典型的性腺,即中腎和主動脈構(gòu)成的空腔管狀結(jié)構(gòu),卻發(fā)現(xiàn)在E10時心臟已經(jīng)發(fā)育,表現(xiàn)為圓球形,內(nèi)部由心肌細(xì)胞及表面的內(nèi)皮細(xì)胞構(gòu)成網(wǎng)狀的空間結(jié)構(gòu),可以看到E10小鼠胚胎已經(jīng)建立循環(huán),血管管腔和心臟內(nèi)部可見少量胞體較大、胞漿豐富的造血干細(xì)胞。E11的心臟組織切片形態(tài)較E10.5并無明顯變化,但此時期心腔內(nèi)的造血細(xì)胞數(shù)量明顯增多,容易見到造血細(xì)胞與心臟內(nèi)皮細(xì)胞接觸。隨著胚胎的發(fā)育,心臟的形態(tài)很快變化,內(nèi)部網(wǎng)狀結(jié)構(gòu)逐漸減少,在E12已具備成體心臟的特點,成為空腔性結(jié)構(gòu)。小鼠E10胚胎中胎肝已經(jīng)發(fā)育,起初為實質(zhì)的結(jié)構(gòu),由胎肝細(xì)胞緊密聯(lián)系而構(gòu)成,內(nèi)部管腔少,隨著胚胎的發(fā)育,胎肝內(nèi)部血管增多。 [結(jié)論]通過E10～E12胚胎正中矢狀面連續(xù)切片和HE染色,并未發(fā)現(xiàn)典型的AGM區(qū)結(jié)構(gòu),可能與組織切片的局限性有關(guān);心臟起初內(nèi)部呈復(fù)雜的空間網(wǎng)狀結(jié)構(gòu),且心肌細(xì)胞表面覆蓋有一層內(nèi)皮細(xì)胞,在造血干細(xì)胞發(fā)育過程中構(gòu)成了巨大的表面積。 第二部分 [目的]研究E10～E12期間小鼠胚胎心臟內(nèi)皮細(xì)胞特性及與造血的發(fā)育相關(guān)的分子機(jī)制。 [方法]將E10～E12小鼠胚胎的組織切片做免疫組化,檢測胚胎心臟是否表達(dá)血管特異性標(biāo)志CD34蛋白;利用已制備好的石蠟切片,通過免疫組化檢測各時間點胚胎心臟和胎肝中Notch信號通路相關(guān)分子,Notch 1和Jagged1蛋白的表達(dá)水平;并且用免疫磁珠分選出c-kit陽性細(xì)胞,通過免疫熒光的方法檢測其細(xì)胞表面Notch 1蛋白的表達(dá)情況。分別取出E10, E10.5, E11, E11.5和E12的胚胎心臟、胎肝和AGM區(qū)組織,經(jīng)機(jī)械碾磨、膠原酶消化和過濾制備單個細(xì)胞懸液,采用差異貼壁法及貼壁培養(yǎng)獲得基質(zhì)細(xì)胞,觀察各組織貼壁細(xì)胞形態(tài),檢測CD31表達(dá)情況。 [結(jié)果] E10～E12的小鼠胚胎心臟的心肌細(xì)胞及其表面的內(nèi)皮細(xì)胞均表達(dá)血管特異性標(biāo)志CD34蛋白,以E11表達(dá)強(qiáng)度最高,同時可以見到循環(huán)的造血細(xì)胞也大多表達(dá)CD34抗原。對組織切片進(jìn)行了Notch信號通路免疫組化的研究,發(fā)現(xiàn)E10心臟心肌細(xì)胞及其表面的內(nèi)皮細(xì)胞形成的中空的網(wǎng)狀結(jié)構(gòu)都表達(dá)Jagged1蛋白和Notch 1蛋白,前者表達(dá)水平高于后者,以E10.5時水平最高,且隨著心臟的發(fā)育和形態(tài)改變,表達(dá)水平逐漸下降,到E12時幾乎消失。同時發(fā)現(xiàn)血管內(nèi)皮細(xì)胞也表達(dá)Jagged1,但是不表達(dá)Notch1蛋白。用c-kit單抗的免疫磁珠分選出c-kit陽性細(xì)胞,通過免疫熒光,顯示這群細(xì)胞表面均勻的表達(dá)Notch 1蛋白。原代心臟貼壁細(xì)胞由體積較大、胞漿豐富、呈不規(guī)則形的心肌細(xì)胞和細(xì)長梭形、胞漿較少的內(nèi)皮細(xì)胞構(gòu)成,容易看到心臟內(nèi)皮細(xì)胞貼附在心肌細(xì)胞表面,呈均一的細(xì)長梭形排列,表達(dá)CD31抗原。胎肝貼壁細(xì)胞形態(tài)相對均一,均為胞體較大、胞漿豐富的細(xì)胞。同期AGM區(qū)貼壁細(xì)胞在原代細(xì)胞中也可見兩個細(xì)胞群體,包括胞體較大的貼壁細(xì)胞和細(xì)長梭形的內(nèi)皮細(xì)胞,形態(tài)與心臟貼壁細(xì)胞相似。 [結(jié)論]胚胎E10～E12期間心臟的心肌細(xì)胞及其內(nèi)皮細(xì)胞均表達(dá)CD34抗原,具備血管內(nèi)皮細(xì)胞特性,網(wǎng)狀結(jié)構(gòu)構(gòu)成巨大的表面積,并且高表達(dá)Jagged1和Notch 1蛋白,形成胚胎造血良好的微環(huán)境,可能參與此時期造血干細(xì)胞的發(fā)育和擴(kuò)增。 第三部分 [目的]E10～E12時期造血的相關(guān)部位Notch信號通路的變化水平及對胚胎中期HSCs擴(kuò)增部位的初探。 [方法]通過實時定量PCR連續(xù)觀察E10, E10.5, E11, E11.5和E12心臟內(nèi)皮細(xì)胞和胎肝基質(zhì)細(xì)胞Notch 1及Jagged1表達(dá)及變化水平。用流式單標(biāo)檢測各時間點心臟和胎肝中c-kit+細(xì)胞比率。將培養(yǎng)24h后的E11心臟懸浮細(xì)胞分別置入由同期的心臟內(nèi)皮細(xì)胞、胎肝基質(zhì)細(xì)胞和AGM區(qū)基質(zhì)細(xì)胞構(gòu)成的共培養(yǎng)體系中,于0h, 24h和48h分別檢測c-kit+細(xì)胞比率,研究各組織基質(zhì)細(xì)胞對HSCs擴(kuò)增的影響。 [結(jié)果]盡管傳代對心臟貼壁細(xì)胞形態(tài)有影響,但是傳代和原代細(xì)胞中Jagged1和Notch 1基因的表達(dá)水平?jīng)]有變化。我們發(fā)現(xiàn):心臟貼壁細(xì)胞的Jagged1基因在E10.5到最高,跟E10的基因水平相比較,為E10的(5.46±2.2)倍,E11時的表達(dá)下降,幾乎與E10相同,但是E11.5和E12時心臟內(nèi)皮細(xì)胞Jagged1表達(dá)均很低,分別為E10的(0.15±0.44)倍和(0.12±0.50)倍。但是胎肝基質(zhì)細(xì)胞Jagged1基因除E11的表達(dá)與E10的心臟內(nèi)皮細(xì)胞相近外,其余胚胎時期均呈低水平表達(dá)。通過流式檢測c-kit+細(xì)胞我們發(fā)現(xiàn),胚胎期心臟和胎肝c-kit+細(xì)胞比率幾乎一致,E11時c-kit+細(xì)胞明顯增多,分別高達(dá)(12.6±3.2)%和(9.6±2.8)%,但E12時心臟中c-kit+細(xì)胞明顯減少,僅為(3.4±1.2)%,此時胎肝中c-kit+細(xì)胞為(11.6±4.1)%。E11的懸浮細(xì)胞與心臟內(nèi)皮細(xì)胞和AGM區(qū)基質(zhì)共培養(yǎng)24h時,c-kit+細(xì)胞數(shù)量保持穩(wěn)定,但在胎肝基質(zhì)細(xì)胞中c-kit+細(xì)胞數(shù)量明顯減少,在共培養(yǎng)48h,三組中的c-kit+細(xì)胞均呈較低水平,組間沒有顯著性差異。 [結(jié)論]心臟內(nèi)皮細(xì)胞中Jagged1基因表達(dá)是變化的,在E10.5時表達(dá)最高,伴隨著c-kit+細(xì)胞數(shù)量的擴(kuò)增。Notch信號通路在胎肝中HSCs的發(fā)育并不起主要作用。通過c-kit+細(xì)胞分別與各種同時期基質(zhì)細(xì)胞共培養(yǎng),推測E11胚胎心臟和AGM區(qū)具備HSCs擴(kuò)增的環(huán)境,E11的胎肝可能并不是造血擴(kuò)增的重要部位。
[Abstract]:Part one
[Objective] to explore the anatomical structure of hematopoietic sites in AGM (E10 ~ E12) mice.
[Methods] the female and male mice were fed with 3: 1 cage to observe the mouse embryos of the exact pregnancy by observing the mouse vaginal suppository, collecting the E10, E10.5, E11, E11.5 and E12 mouse embryos, removing the placenta and placenta, using 4% formaldehyde to fix the 3h, the ethanol was dehydrated, the paraffin was embedded and stained through the median sagittal plane of embryo fetal, and HE staining was carried out under the microscope. Under the microscope, the microscope was continuous under the microscope. Under the microscope, the microscope was continuous under the microscope. Under the microscope, continuous microscope was continuous under microscope. Under microscope, continuous microscope was continuous under microscope. Under microscope, continuous microscope was continuous under microscope. Under microscope, continuous microscope was continuous under microscope. Under microscope, continuous microscope was continuous under microscope. Under microscope, continuous microscope was continuous under microscope Observe the structure of the hematopoietic tissue.
[results] the median sagittal section of the embryo, from E10 to E12, did not see the typical gonad on the dorsal side of the abdomen, that is, the cavity tubular structure of the middle kidney and the aorta, but found that the heart developed at the time of E10, showing a circular sphere, and the internal structure of the endothelium of the myocardium and the surface of the endothelial cells, which could be seen in the E10 mouse embryo. A small number of cells in the vascular lumen and heart can be seen in the fetus. There is no obvious change in the morphology of the cardiac tissue section of.E11 with abundant cytoplasmic hematopoietic stem cells than that of E10.5. However, the number of hematopoietic cells in the heart cavity increases significantly at this time, and it is easy to see the contact between the hematopoietic cells and the inner heart endothelial cells. With the development of the embryo, the heart is developed. The shape of the internal reticular formation is gradually reduced, and the internal reticular structure is gradually reduced, and the E12 has the characteristics of the adult heart and becomes a cavity structure. The fetal liver of the mouse E10 embryo is developed at first, which is a solid structure, which is made up of the fetal liver cells closely connected, and the internal cavities are few. With the development of the embryo, the blood vessels in the fetal liver increase.
[Conclusion] the typical AGM region structure was not found through continuous slicing in the median sagittal plane of E10 to E12 embryos and HE staining. It may be related to the limitation of the tissue section. At first, the heart had a complex spatial network structure and a layer of endothelial cells covered the surface of the cardiac myocytes, which formed a huge surface during the development of hematopoietic stem cells. Product.
Part two
[Objective] to study the characteristics of mouse embryonic heart endothelial cells during E10 ~ E12 and the molecular mechanism related to hematopoietic development.
[Methods] the tissue section of E10 ~ E12 mouse embryos was immunohistochemical, and the blood vessel specific marker CD34 protein was detected by the embryo heart. By using the prepared paraffin section, the expression level of Notch signaling pathway related molecules in the fetal heart and fetal liver, the expression level of Notch 1 and Jagged1 protein were detected by immunohistochemistry. C-kit positive cells were selected by immunomagnetic beads. The expression of Notch 1 protein on the surface of the cells was detected by immunofluorescence. The embryonic heart of E10, E10.5, E11, E11.5 and E12, fetal liver and AGM region tissues were prepared by mechanical milling, collagenase digestion and filtration to prepare single cell suspension. The differential adherence method and adherent culture were used. The stromal cells were observed, the morphology of adherent cells in each tissue was observed, and the expression of CD31 was detected.
[results] the cardiac myocytes and the endothelial cells on the surface of the mouse embryonic heart of E10 ~ E12 all express the vascular specific marker CD34 protein, and the expression of E11 is the highest, while the circulating hematopoietic cells can also express most of the CD34 antigens. The immunohistochemical study of Notch signal in the tissue sections has been carried out to detect the cardiac muscle fine of E10. The hollow reticular formation of endothelial cells on the cell and its surface all express Jagged1 protein and Notch 1 protein. The expression level of the former is higher than that of the latter, with the highest level at E10.5, and with the development and morphologic changes of the heart, the expression level gradually decreases, and it is almost disappeared at E12. It is found that the vascular endothelial cells also express Jagged1, but not at the same time. Expression of Notch1 protein. C-kit positive cells were separated by the immunomagnetic beads of c-kit monoclonal antibody. By immunofluorescence, the expression of Notch 1 protein on the surface of the group was revealed. The primary cardiac parietal cells were composed of large volume, rich cytoplasm, irregular cardiomyocytes, elongated spindle shaped, less cytoplasmic endothelial cells, and easy to see the heart. The endothelial cells were attached to the surface of the cardiac myocytes, showing a uniform elongated spindle arrangement and expression of CD31 antigen. The morphology of the fetal liver adherent cells was relatively uniform, both of which were larger and rich in cytoplasm. At the same time, two cell groups were also found in the primary cells, including the larger cell adherent cells and spindly spindle endothelial cells in the AGM area. The morphology is similar to that of the cardiac parietal cells.
[Conclusion] the cardiac myocytes and endothelial cells of the heart from E10 to E12 all express CD34 antigen, and possess the characteristics of vascular endothelial cells, the reticular structure constitutes a huge surface area, and the high expression of Jagged1 and Notch 1 proteins to form a good microenvironment for embryo hematopoiesis, which may be involved in the development and expansion of hematopoietic stem cells at this time.
The third part
[Objective] to investigate the level of Notch signaling pathway in hematopoietic related parts from]E10 to E12 and to explore the location of HSCs amplification in the middle stage of embryo.
[Methods] the expression and change levels of E10, E10.5, E11, E11.5 and E12 cardiac endothelial cells and fetal liver stromal cells Notch 1 and Jagged1 were continuously observed by real-time quantitative PCR. The ratio of c-kit+ cells in the heart and fetal liver at each time point was detected by flow single standard. The E11 cardiac suspension cells after the culture of 24h were placed in the same period of cardiac endothelial cells, respectively. In the co culture system of fetal liver stromal cells and AGM region stromal cells, the ratio of c-kit+ cells was detected by 0h, 24h and 48h, and the effect of matrix cells on the amplification of HSCs was studied.
[results] the expression level of Jagged1 and Notch 1 genes in the passages and primary cells did not change in spite of the influence on the morphology of the cardiac parietal cells. We found that the Jagged1 gene of the heart adherent cells was the highest in E10.5, compared with the E10 gene level, which was (5.46 + 2.2) times of that of E10, and the expression of E11 decreased, almost the same as E10. But the expression of Jagged1 in E11.5 and E12 was very low, which was E10 (0.15 + 0.44) times and (0.12 + 0.50) times respectively. But the expression of Jagged1 gene in fetal liver stromal cells, except E11, was similar to that of E10's heart endothelial cells. All the other embryonic stages were low level expression. Through flow cytometry, we found that the embryonic heart was the heart of c-kit+ cells. The ratio of c-kit+ cells to fetal liver was almost identical, and the number of c-kit+ cells increased significantly at the time of E11, up to (12.6 + 3.2)% and (9.6 + 2.8)% respectively, but the c-kit+ cells in the heart decreased significantly at the time of E12, only (3.4 + 1.2)%. At this time, c-kit+ cells in fetal liver (11.6 + 4.1)%.E11 were co cultured 24h with cardiac endothelial cells and AGM region matrix, and the number of c-kit+ cells The amount of c-kit+ cells in the fetal liver stromal cells decreased significantly, and in co culture of 48h, the c-kit+ cells in the three groups were all low, and there was no significant difference between the groups.
[Conclusion] the expression of Jagged1 gene in the cardiac endothelial cells is changed and the expression is highest at E10.5. The development of HSCs in fetal liver is not the main function of the amplification of.Notch signal pathway with the number of c-kit+ cells. C-kit+ cells are co cultured with a variety of simultaneous stromal cells, and the E11 embryo heart and AGM region have HSCs amplification. Environment, fetal liver of E11 may not be an important part of hematopoietic expansion.

【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2009
【分類號】：R329

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 甘志華;陳鈺;;Notch信號通路和多發(fā)性骨髓瘤[J];中國實驗血液學(xué)雜志;2009年05期

2 甘志華;陳鈺;閻驊;王侃侃;;人Jagged-1蛋白在真核細(xì)胞中的表達(dá)及穩(wěn)定株的建立[J];中國實驗血液學(xué)雜志;2010年04期

3 鞠桂芝;纖維酯膜(CEM)上的造血 (Ⅰ)造血微環(huán)境細(xì)胞的功能特征[J];國際輸血及血液學(xué)雜志;1978年01期

4 楊崇禮;;造血微環(huán)境——文獻(xiàn)綜述[J];國際輸血及血液學(xué)雜志;1983年03期

5 陳春華;舍英;;細(xì)胞化學(xué)觀察體外培養(yǎng)骨髓基質(zhì)細(xì)胞的羥脯氨酸-2-表異構(gòu)酶活性[J];內(nèi)蒙古醫(yī)學(xué)院學(xué)報;1990年01期

6 葛志紅,趙英;成纖維祖細(xì)胞的培養(yǎng)及其意義[J];青海醫(yī)學(xué)院學(xué)報;1995年02期

7 劉真;蔣天倫;范婭涵;趙樹銘;黎儒青;;豬造血微環(huán)境體外模型對人HSC分化作用的實驗研究[J];第三軍醫(yī)大學(xué)學(xué)報;2006年23期

8 張曦;司英健;陳幸華;劉耀;高蕾;高力;彭賢貴;王慶余;;裸鼠造血微環(huán)境損傷實驗動物模型的建立[J];西部醫(yī)學(xué);2008年01期

9 程益華;王娟;陳秀華;;Notch信號通路在腫瘤干細(xì)胞中的作用及其抑制劑研究[J];世界臨床藥物;2010年10期

10 高樝;肖揚(yáng);蔣祖軍;李力;李勇華;張小明;李利;龐妍;歐陽玲;;骨髓間充質(zhì)干細(xì)胞治療再生障礙性貧血的療效及安全性[J];廣東醫(yī)學(xué);2010年21期

相關(guān)會議論文 前10條

1 周詠春;李康樗;張杰;劉軍葉;郭國禎;;EMP對小鼠胚胎肢芽細(xì)胞的影響[A];中國毒理學(xué)會第二屆全國中青年學(xué)者科技論壇會議論文集[C];2007年

2 謝輝蓉;夏國良;卜淑敏;雷蕾;汪建紅;;促減數(shù)分裂甾醇可能參與調(diào)節(jié)小鼠胚胎卵巢卵泡發(fā)生[A];中國生理學(xué)會第21屆全國代表大會暨學(xué)術(shù)會議論文摘要匯編[C];2002年

3 徐平;;實驗大鼠、小鼠胚胎的緩慢冷凍和玻璃化冷凍的比較研究[A];中國實驗動物學(xué)會青年科技協(xié)會第二屆學(xué)術(shù)研討會論文匯編[C];2001年

4 劉烈剛;劉恭平;姚平;郝麗萍;楊雪峰;孫秀發(fā);朱清華;;維生素A缺乏對小鼠胚胎Hox-3.5mRNA表達(dá)的影響及其與胚胎發(fā)育關(guān)系的研究[A];中國營養(yǎng)學(xué)會第九次全國營養(yǎng)學(xué)術(shù)會議論文摘要匯編[C];2004年

5 孔英;石英;陳巍;燕秋;朱正美;;小鼠胚胎Le~Y寡糖抗原的分泌及其功能意義[A];第八屆全國復(fù)合糖生物化學(xué)與分子生物學(xué)學(xué)術(shù)會議論文摘要論文集[C];2004年

6 謝利劍;黃國英;趙曉晴;齊春華;彭濤;周國民;;Cx40、Cx45在Cx43基因敲除小鼠胚胎心臟時表達(dá)的研究[A];中華醫(yī)學(xué)會第十四次全國兒科學(xué)術(shù)會議論文匯編[C];2006年

7 倪華;;小鼠胚胎著床的分子調(diào)控[A];中國生理學(xué)會張錫鈞基金會第八屆全國青年優(yōu)秀生理學(xué)學(xué)術(shù)論文綜合摘要[C];2003年

8 謝利劍;黃國英;趙曉晴;齊春華;彭濤;周國民;;Cx40、Cx45在Cx43基因敲除小鼠胚胎心臟時空表達(dá)的研究[A];2006（第三屆）江浙滬兒科學(xué)術(shù)會議暨浙江省兒科學(xué)術(shù)年會論文匯編[C];2006年

9 邢巖江;谷甜甜;黃治軍;賀洪娟;吳瓊;;UbcM2基因在小鼠胚胎中的表達(dá)分析[A];“細(xì)胞活動 生命活力”——中國細(xì)胞生物學(xué)學(xué)會全體會員代表大會暨第十二次學(xué)術(shù)大會論文摘要集[C];2011年

10 黃麗芳;周琨;胡采紅;王芳;孫漢英;劉文勵;;E10～E12期間小鼠胚胎心臟內(nèi)皮細(xì)胞特性及與造血干細(xì)胞擴(kuò)增相關(guān)分子變化水平的研究[A];第12屆全國實驗血液學(xué)會議論文摘要[C];2009年

相關(guān)重要報紙文章 前10條

1 北京軍區(qū)總醫(yī)院 教授 張禹;胃的解剖結(jié)構(gòu)[N];家庭醫(yī)生報;2003年

2 北京軍區(qū)總醫(yī)院內(nèi)科 教授 張禹;食管的解剖結(jié)構(gòu)[N];家庭醫(yī)生報;2003年

3 李曉紅;慢性再障的三步療法[N];健康報;2006年

4 楊孝文 任秋凌;人類“走出非洲”嗎？[N];中國國土資源報;2005年

5 遠(yuǎn)深;神經(jīng)內(nèi)鏡“排通”腦積水顯神奇[N];中國醫(yī)藥報;2008年

6 河北醫(yī)大附屬以嶺醫(yī)院醫(yī)學(xué)博士 李曉紅;慢性再生障礙性貧血的“三步療法”[N];健康報;2008年

7 尹立雪;讓心臟完整展現(xiàn)[N];健康報;2006年

8 熊和生邋俞楠;我省學(xué)者探究中醫(yī)學(xué)氣質(zhì)原理[N];貴州日報;2008年

9 李七渝 張紹祥;關(guān)鍵技術(shù)：步步為營[N];計算機(jī)世界;2003年

10 保健時報實習(xí)記者 王朝;治三尖瓣下移靠“裁縫”[N];保健時報;2007年

相關(guān)博士學(xué)位論文 前10條

1 黃麗芳;小鼠胚胎E10～E12造血擴(kuò)增部位的探尋及其分子作用機(jī)制的研究[D];華中科技大學(xué);2009年

2 蘇永鋒;間充質(zhì)干細(xì)胞調(diào)控選擇性活化樹突狀細(xì)胞發(fā)育[D];中國人民解放軍軍事醫(yī)學(xué)科學(xué)院;2009年

3 田蕾;Adam10基因條件剔除小鼠的培育及ADAM10在胸腺細(xì)胞發(fā)育中調(diào)控Notch信號通路的研究[D];復(fù)旦大學(xué);2007年

4 李劍平;Notch信號通路在同基因造血干/祖細(xì)胞移植誘導(dǎo)免疫耐受中的作用[D];第四軍醫(yī)大學(xué);2008年

5 杜宏;Notch及相關(guān)信號傳導(dǎo)體系對細(xì)胞增殖及分化的調(diào)控作用[D];中國協(xié)和醫(yī)科大學(xué);2008年

6 史濤坪;Ⅰ. Notch信號通路基因在膀胱癌中的表達(dá)及預(yù)后分析研究 Ⅱ. 早期留置雙J管在腎結(jié)核治療中的臨床應(yīng)用研究[D];華中科技大學(xué);2009年

7 鈕煒西;小鼠胚胎臍血管的造血功能研究[D];第三軍醫(yī)大學(xué);2012年

8 譚著明;紅汁乳菇生物學(xué)特性與半人工栽培技術(shù)研究[D];湖南農(nóng)業(yè)大學(xué);2005年

9 井然;Cypher和ENH在小鼠早期心臟發(fā)育中的作用及其分子機(jī)制[D];中南大學(xué);2012年

10 劉世彪;絞股藍(lán)結(jié)構(gòu)和發(fā)育與其總皂甙積累相關(guān)性的研究[D];西北大學(xué);2005年

相關(guān)碩士學(xué)位論文 前10條

1 謝蒼桑;DAPT抑制Notch信號通路對神經(jīng)母細(xì)胞瘤細(xì)胞增殖的影響[D];福建醫(yī)科大學(xué);2009年

2 佘林;Notch信號通路在涎腺腺樣囊性癌中的表達(dá)及意義[D];福建醫(yī)科大學(xué);2009年

3 孫明洲;用結(jié)構(gòu)學(xué)方法對中國北方鳶尾屬植物的分類學(xué)研究[D];東北師范大學(xué);2004年

4 韋麗君;部分蘇鐵類植物的解剖結(jié)構(gòu)特征及其與環(huán)境的適應(yīng)性研究[D];廣西大學(xué);2005年

5 李萼;降塵對棉花生長發(fā)育的影響[D];湖南農(nóng)業(yè)大學(xué);2005年

6 季青;華山新麥草營養(yǎng)器官的解剖學(xué)研究[D];西北農(nóng)林科技大學(xué);2004年

7 馮國祿;爬山虎的形態(tài)解剖結(jié)構(gòu)觀察[D];湖南農(nóng)業(yè)大學(xué);2007年

8 陳敏瑩;微螺釘種植體植入?yún)^(qū)域頜骨解剖的CBCT測量分析[D];暨南大學(xué);2008年

9 趙蕾;野生歐洲李抗寒特性研究[D];新疆農(nóng)業(yè)大學(xué);2009年

10 鄭鶯鳳;TGF-α誘導(dǎo)人神經(jīng)膠質(zhì)瘤U251 Notch靶基因Hes1的表達(dá)[D];福建醫(yī)科大學(xué);2007年

，

本文編號：1801355