發(fā)布時(shí)間：2018-04-17 15:27

  本文選題：熱量限制 + SIRT抑制劑�。� 參考：《首都醫(yī)科大學(xué)學(xué)報(bào)》2015年05期

【摘要】：目的觀察SIRT1抑制劑EX527對(duì)熱量限制處理的人神經(jīng)母細(xì)胞瘤株SH-SY5Y細(xì)胞的影響。方法體外培養(yǎng)SHSY5Y細(xì)胞,分為正常對(duì)照組(NC組)、熱量限制組(CR組)和熱量限制加SIRT1抑制劑組(CR+EX527組),光鏡下觀察細(xì)胞形態(tài)學(xué)改變,測(cè)定各組細(xì)胞噻唑藍(lán)(thiazolyl blue tetrazolium bromide,MTT)代謝率及乳酸脫氫酶(lactate dehydrogenase,LDH)漏出率,應(yīng)用蛋白免疫印跡(Western blotting)法測(cè)定SIRT1表達(dá)量。結(jié)果與NC組相比,CR組細(xì)胞形態(tài)好,表現(xiàn)為細(xì)胞胞體飽滿、突起伸展、貼壁牢固,MTT代謝率升高(P0.05),LDH漏出率有降低趨勢(shì),CR組和CR+EX527組SIRT1表達(dá)均增加(P0.05);與CR組相比,CR+EX527組細(xì)胞形態(tài)較差,突起回縮,貼壁性不好,MTT代謝率降低(P0.05),LDH漏出率有升高趨勢(shì),SIRT1表達(dá)略減少。結(jié)論熱量限制對(duì)神經(jīng)細(xì)胞具有保護(hù)作用,而SIRT1抑制劑EX527能夠減弱熱量限制的神經(jīng)保護(hù)作用,提示熱量限制對(duì)SH-SY5Y細(xì)胞的保護(hù)作用可能是由SIRT1介導(dǎo)的。
[Abstract]:Objective to observe the effect of SIRT1 inhibitor EX527 on human neuroblastoma cell line SH-SY5Y treated with heat restriction.Methods SHSY5Y cells were cultured in vitro and divided into normal control group (NC group), caloric restriction group (CR group) and caloric restriction plus SIRT1 inhibitor group (CR EX527 group). Morphological changes were observed under light microscope.The metabolic rate of thiazolyl blue tetrazolium bromidede (MTT) and the leakage rate of lactate dehydrogenase (LDH) were measured. The expression of SIRT1 was detected by Western blotting.Results compared with NC group, the cell morphology of CR group was better than that of NC group, which showed that the cell body was plump, the protuberance was extended, the metabolic rate of adherent to the cell wall was increased, the leakage rate of SIRT1 was decreased in CR group and CR EX527 group, the expression of SIRT1 in CR group and CR EX527 group was higher than that in CR group, and the cell morphology in CR EX527 group was worse than that in CR group.Protruding back, MTT metabolic rate decreased, the leakage rate of LDH increased and the expression of SIRT1 decreased slightly.Conclusion caloric restriction has protective effect on nerve cells, while SIRT1 inhibitor EX527 can attenuate the neuroprotective effect of caloric restriction on SH-SY5Y cells, suggesting that the protective effect of caloric restriction on SH-SY5Y cells may be mediated by SIRT1.
【作者單位】： 首都醫(yī)科大學(xué)宣武醫(yī)院中心實(shí)驗(yàn)室;北京市老年病醫(yī)療研究中心;北京腦重大疾病研究院阿爾茨海默病研究所;神經(jīng)變性病教育部重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金(81472007) 北京市自然科學(xué)基金(7132044)~~
【分類號(hào)】：R329.2

【參考文獻(xiàn)】

相關(guān)期刊論文 前4條

1 趙靜姝;王蓉;張景燕;吳燕川;姬志娟;;不同能量的培養(yǎng)條件對(duì)人神經(jīng)母細(xì)胞瘤株SH-SY5Y細(xì)胞生長(zhǎng)的影響[J];中國(guó)比較醫(yī)學(xué)雜志;2012年02期

2 陳娟;張景燕;王蓉;趙靜姝;郭瑾;吳燕川;趙志煒;;熱量限制對(duì)SH-SY5Y細(xì)胞氧化損傷的影響[J];中國(guó)比較醫(yī)學(xué)雜志;2012年10期

3 王聰杰;牛平;陳鑫;劉寶茹;辛志強(qiáng);;白藜蘆醇對(duì)帕金森病大鼠黑質(zhì)多巴胺能神經(jīng)元損傷的保護(hù)作用[J];中國(guó)醫(yī)科大學(xué)學(xué)報(bào);2012年01期

4 趙紅宇;蔡煒嵩;李帥;笪祖科;孫寒雪;馬良;林耀新;智德豹;;人類神經(jīng)母細(xì)胞瘤骨轉(zhuǎn)移模型的建立及鑒定[J];中國(guó)醫(yī)科大學(xué)學(xué)報(bào);2013年02期

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 馬麗娜;王蓉;;熱量限制與沉默信息調(diào)節(jié)因子2相關(guān)酶1[J];中華老年心腦血管病雜志;2013年11期

2 游娜;王雅云;董成龍;馮亞敏;繆珩;丁大法;魯一兵;劉超;;白藜蘆醇對(duì)高糖狀態(tài)下大鼠腎小管上皮細(xì)胞增殖、凋亡的影響及機(jī)制[J];南京醫(yī)科大學(xué)學(xué)報(bào)(自然科學(xué)版);2015年04期

3 史小翠;馮小燕;宋然;楊龍燕;賀俊崎;;帕金森大鼠消化道組織中蛋白質(zhì)NHERF家族和CAL的表達(dá)變化[J];首都醫(yī)科大學(xué)學(xué)報(bào);2013年02期

4 李加興;吳越;陳選;涂媛;周炎輝;;多烯酸植物油及其保健功效研究進(jìn)展[J];食品科學(xué);2014年21期

5 趙志煒;馬麗娜;王蓉;張旭;王玉蘭;吳燕川;陳娟;張景燕;;熱量限制對(duì)C57小鼠學(xué)習(xí)記憶能力及胰島素信號(hào)通路相關(guān)蛋白的影響[J];首都醫(yī)科大學(xué)學(xué)報(bào);2014年06期

6 金玲;金香淑;;紅參皂苷Rg5對(duì)MPP~+所致SH-SY5Y細(xì)胞氧化應(yīng)激損傷的保護(hù)作用[J];時(shí)珍國(guó)醫(yī)國(guó)藥;2015年04期

7 趙志煒;馬麗娜;王蓉;張旭;陳娟;張景燕;;熱量限制對(duì)C57小鼠糖脂代謝的影響[J];中國(guó)比較醫(yī)學(xué)雜志;2013年08期

8 張忠霞;馬曉偉;顧平;李曉麗;王彥永;王銘維;;年齡對(duì)MPTP作用于快速老化小鼠SAMP8環(huán)氧化酶2表達(dá)的影響[J];中國(guó)醫(yī)科大學(xué)學(xué)報(bào);2013年08期

9 王雨o，

本文編號(hào)：1764172