本文選題：小鼠 + 外周血單核巨噬細(xì)胞��； 參考：《首都醫(yī)科大學(xué)學(xué)報(bào)》2015年04期

【摘要】：目的在L929條件培養(yǎng)基的作用下體外分離、培養(yǎng)小鼠外周血單核巨噬細(xì)胞,并進(jìn)行鑒定。方法密度梯度離心法分離小鼠外周血單核巨噬細(xì)胞,用L929條件培養(yǎng)基培養(yǎng)7 d。采用免疫熒光的方法檢測(cè)F4/80的表達(dá)以及細(xì)胞的吞噬作用,用流式細(xì)胞術(shù)進(jìn)一步檢測(cè)細(xì)胞吞噬率。結(jié)果用L929條件培養(yǎng)基誘導(dǎo)培養(yǎng)7 d后小鼠外周血單核細(xì)胞表達(dá)F4/80,加入細(xì)胞吞噬珠之后,在不同時(shí)間點(diǎn)用熒光顯微鏡可以看到紅色熒光顆粒聚集在小鼠外周血單核巨噬細(xì)胞核周圍,用流式細(xì)胞儀檢測(cè)細(xì)胞的吞噬率為24.8%±0.79%。結(jié)論該方法是一種簡(jiǎn)單、易操作的體外分離培養(yǎng)和鑒定小鼠外周血單核巨噬細(xì)胞的方法。
[Abstract]:Objective to isolate and identify murine peripheral blood mononuclear macrophages in L929 conditioned medium.Methods Mouse peripheral blood mononuclear macrophages were isolated by density gradient centrifugation and cultured on L929 conditioned medium for 7 days.The expression of F4 / 80 and the phagocytosis of F4 / 80 were detected by immunofluorescence, and the phagocytosis rate was further detected by flow cytometry.Results the peripheral blood monocytes of mice were induced to express F4 / 80 in L929 conditioned medium for 7 days, and the cells were added to phagocytic beads.At different time points, red fluorescent particles gathered around the nucleus of mononuclear macrophages in peripheral blood of mice, and the phagocytosis rate of the cells detected by flow cytometry was 24.8% 鹵0.79.Conclusion this method is a simple and easy to operate method for isolation, culture and identification of mouse peripheral blood mononuclear macrophages.
【作者單位】： 首都醫(yī)科大學(xué)細(xì)胞生物學(xué)系肝臟保護(hù)與再生調(diào)節(jié)北京市重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金(81430013) 北京市屬高等學(xué)校創(chuàng)新團(tuán)隊(duì)建設(shè)與教師職業(yè)發(fā)展計(jì)劃項(xiàng)目(IDHT20150502)~~
【分類號(hào)】：R392

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 章必成;高建飛;;M2型巨噬細(xì)胞:腫瘤治療的新靶點(diǎn)[J];臨床誤診誤治;2012年04期

2 穆s，

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