本文選題：導(dǎo)肽　切入點(diǎn)：線(xiàn)粒體　出處：《浙江大學(xué)》2009年博士論文　論文類(lèi)型：學(xué)位論文

【摘要】：氫化酶體是厭氧原蟲(chóng)或真菌細(xì)胞內(nèi)的亞細(xì)胞結(jié)構(gòu),可通過(guò)丙酮酸代謝產(chǎn)ATP和分子氫,現(xiàn)有的研究認(rèn)為氫化酶體與線(xiàn)粒體具有共同的起源。蛋白組分的確定對(duì)深入了解質(zhì)體的生化代謝和進(jìn)化過(guò)程有重要的意義。雖然目前已有多種分子生物學(xué)、蛋白組學(xué)和計(jì)算預(yù)測(cè)的方法應(yīng)用于線(xiàn)粒體蛋白的定位分析中,但氫化酶體相關(guān)領(lǐng)域的研究進(jìn)展卻相對(duì)緩慢。本文在氫化酶體和線(xiàn)粒體進(jìn)化關(guān)系和蛋白轉(zhuǎn)運(yùn)機(jī)制的研究基礎(chǔ)上,通過(guò)歸納線(xiàn)粒體蛋白與氫化酶體蛋白導(dǎo)肽共有的生物學(xué)特征,運(yùn)用簡(jiǎn)單規(guī)則和特異性模式構(gòu)建與搜索的方法,對(duì)陰道滴蟲(chóng)的氫化酶體蛋白進(jìn)行預(yù)測(cè),為進(jìn)一步通過(guò)實(shí)驗(yàn)方法鑒定氫化酶體蛋白提供了有效的候選蛋白。 應(yīng)用本文提出的HdPred方法共預(yù)測(cè)獲得70個(gè)可能的氫化酶體蛋白,對(duì)該結(jié)果的深入評(píng)價(jià)發(fā)現(xiàn),其中50%的組分通過(guò)實(shí)驗(yàn)方法或在其它預(yù)測(cè)方法得到了確認(rèn),表明該方法能較為有效地對(duì)氫化酶體蛋白進(jìn)行富集。本文的研究報(bào)道了多個(gè)能量代謝相關(guān)的氫化酶體蛋白,進(jìn)一步說(shuō)明了氫化酶體在能量代謝過(guò)程中的重要作用,同時(shí)本文的方法發(fā)現(xiàn)氫化酶體中可能具有的脂肪酸代謝相關(guān)的組分,為全面了解該質(zhì)體的生化代謝途徑提供了明確的目標(biāo)基因。此外,通過(guò)比較不同方法對(duì)導(dǎo)肽序列的預(yù)測(cè)結(jié)果,顯示本文采用的方法在導(dǎo)肽序列的預(yù)測(cè)上具有更高的準(zhǔn)確性。 本文首次全面地對(duì)線(xiàn)粒體蛋白導(dǎo)肽的起源和進(jìn)化方式進(jìn)行歸納,并按照其起源方式分為外源性獲得和自身起源兩個(gè)類(lèi)型。在蛋白質(zhì)組學(xué)對(duì)陰道滴蟲(chóng)的氫化酶體蛋白鑒定結(jié)果的基礎(chǔ)上,本文對(duì)其中Hsp60蛋白的導(dǎo)肽的起源和進(jìn)化進(jìn)行了探討,分析表明其導(dǎo)肽可能通過(guò)基因水平上的復(fù)制獲得,從而對(duì)現(xiàn)有關(guān)于導(dǎo)肽起源的研究進(jìn)行了有益的補(bǔ)充。
[Abstract]:Hydrogenosome is anaerobic protozoa or fungi subcellular structures within the cell, through the pyruvate metabolism and molecular hydrogen production of ATP, the existing research that the hydrogenosomes and mitochondria had a common origin. Protein components of certain understanding has important significance of biochemical metabolism and evolution of plastids. Although there have been a variety of molecular biology location analysis, proteomics and computational prediction method is applied to the mitochondrial protein, but the research progress of hydrogenosomes in related fields is relatively slow. In this paper, based on the transfer mechanism in hydrogenosomes and evolutionary relationship of the mitochondrial protein and, through the induction of mitochondrial protein and hydrogenase protein peptide common biologic guide the characteristics, use simple rules and specific mode and search method of hydrogenosomes protein on Trichomonas vaginalis predicted by experimental method for further reference The hydrogenated enzyme protein provides an effective candidate protein.
HdPred using the method presented in this paper is obtained to predict the 70 possible hydrogenosomes protein, found in-depth evaluation of the results, of which 50% of the components by experimental methods or other prediction methods in the confirmation, show that this method can be effective to hydrogenosomes in protein enrichment. This research reports a plurality of energy metabolism related protein hydrogenosomes, further demonstrated the important role of hydrogenosomes in energy metabolism, and this method may have found hydrogenosomes in fatty acid metabolism related components, provides a clear target gene to understand the biochemical metabolic pathways of the body. In addition, through the comparison of different methods to guide peptide sequence prediction. The results show this method has higher accuracy in predicting the guide peptide sequence.
For the first time full of mitochondrial origin and evolution of peptides were summarized, and according to their mode of origin is divided into exogenous and its origin two types. Based on vaginal trichomonad hydrogenosomes protein identification results in proteomics, the origin and evolution of leader peptide of Hsp60 protein has carried on the discussion, analysis showed that the peptide may guide through genetic level replication, which is a useful supplement to the existing research on the origin of the guide peptide.

【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2009
【分類(lèi)號(hào)】：R341

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