發(fā)布時間：2018-03-11 07:21

  本文選題：免疫耐受　切入點：CD45RB抗體　出處：《暨南大學(xué)》2010年碩士論文　論文類型：學(xué)位論文

【摘要】： 目的：研究抗CD45RB抗體在誘導(dǎo)免疫耐受中對不同T淋巴細(xì)胞亞群的影響,以闡明T細(xì)胞亞群在抗CD45RB抗體誘導(dǎo)的免疫耐受中可能發(fā)揮的作用機制。 方法：將T淋巴細(xì)胞與ConA或抗CD45RB抗體共培養(yǎng)72小時,檢測淋巴細(xì)胞增殖被抑制能力。取BALB/c鼠脾細(xì)胞作為刺激細(xì)胞,與C57BL/6鼠T淋巴細(xì)胞或抗CD45RB抗體共培養(yǎng)72小時后收集混合細(xì)胞和培養(yǎng)上清。建立小鼠心臟移植模型,觀察抗CD45RB抗體對移植物生存期的影響。在移植術(shù)后第0、1、3、5、7天時取外周血、脾臟組織和移植心臟。流式細(xì)胞術(shù)分析混合細(xì)胞和外周血中Th1 (CD3+CD8"IFN-y+)、Th2 (CD3+CD8'IL-4+)、Treg (CD4+CD25+CD127-)、Ts(CD8+CD28")、Tm(CD4+CD62L-CD44hi)變化；ELISA法檢測培養(yǎng)上清和血清中IL-2、IL-4、IL-10、IFN-y含量；Real-Time PCR法檢測混合細(xì)胞和脾臟組織Foxp3 mRNA;病理學(xué)觀察移植心臟組織學(xué)變化。 結(jié)果：抗CD45RB抗體顯著延長移植物存活時間,對ConA刺激引起的T淋巴細(xì)胞增殖具有明顯的抑制能力。與對照組相比,抗CD45RB抗體能明顯上調(diào)混合細(xì)胞和外周血中Th2細(xì)胞、Treg細(xì)胞和TS細(xì)胞,下調(diào)Th1細(xì)胞和Tm細(xì)胞；可使細(xì)胞因子IL-2和IFN-γ的水平降低,IL-4和IL-10的含量升高；增加Foxp3 mRNA的表達(dá)量。病理學(xué)顯示對照組移植心臟出現(xiàn)典型細(xì)胞免疫性損傷病理改變,而實驗組中幾乎無炎性細(xì)胞浸潤現(xiàn)象。 結(jié)論：抗CD45RB抗體能顯著明顯延長移植物存活時間,其誘導(dǎo)免疫耐受可能與上調(diào)Th2細(xì)胞、Treg細(xì)胞和TS細(xì)胞,下調(diào)Th1細(xì)胞和Tm細(xì)胞,增加Foxp3 mRNA表達(dá)量有關(guān)。
[Abstract]:Aim: to study the effect of anti CD45RB antibody on different T lymphocyte subsets in inducing immune tolerance, and to elucidate the role of T cell subsets in the immune tolerance induced by anti CD45RB antibody. Methods: t lymphocytes were co-cultured with ConA or anti CD45RB antibody for 72 hours to detect the ability of inhibiting lymphocyte proliferation. Spleen cells of BALB/c mice were used as stimulators. Mixed cells and supernatants were collected and cultured after 72 hours co-culture with C57BL / 6 T lymphocytes or anti CD45RB antibody. The mouse heart transplantation model was established to observe the effect of anti CD45RB antibody on graft survival. Splenic tissue and heart grafts. Flow cytometry analysis of the changes of Th1 T cell line CD3 CD8 "IFN-y Th _ 2 T _ 3 CD8'IL-4" T _ 4 CD25 CD _ (127-T) CD _ (8) CD28 "T _ m T _ m CD _ 4 ~ + CD _ 62L-CD44hi. Elisa for the detection of IL-2IL-4IL-10IL-10IFN-y in culture supernatant and serum; Real-Time PCR method) for detection of Foxp3 in mixed and splenic tissues. MRNAs; histopathological changes of transplanted heart. Results: anti CD45RB antibody significantly prolonged the survival time of grafts and significantly inhibited the proliferation of T lymphocytes stimulated by ConA. Anti CD45RB antibody could significantly up-regulate Th2 cells, Treg cells and TS cells, down-regulate Th1 cells and TM cells, decrease the levels of cytokines IL-2 and IFN- 緯 and increase the contents of IL-4 and IL-10 in mixed cells and peripheral blood cells. The expression of Foxp3 mRNA was increased and the pathological changes of typical cellular immunological injury were observed in the control group, but there were almost no inflammatory cell infiltration in the experimental group. Conclusion: anti CD45RB antibody can significantly prolong the survival time of grafts and its induction of immune tolerance may be related to up-regulating the expression of Foxp3 mRNA in Th2 cells and TS cells down-regulating Th1 cells and TM cells and increasing the expression of Foxp3 mRNA.
【學(xué)位授予單位】：暨南大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R392

【參考文獻】

相關(guān)期刊論文 前2條

1 王繼納;朱同玉;;藥物在誘導(dǎo)臨床移植免疫耐受中的應(yīng)用[J];中國臨床藥學(xué)雜志;2007年05期

2 ;Changes of CD8+CD28-T regulatory cells in rat model of colitis induced by 2,4-dinitrofluorobenzene[J];World Journal of Gastroenterology;2003年11期

，

本文編號：1597135