發(fā)布時間：2018-03-10 00:04

  本文選題：miR-基因敲減　切入點(diǎn)：腸系膜淋巴結(jié)　出處：《中國免疫學(xué)雜志》2015年09期 　論文類型：期刊論文

【摘要】：目的:研究miR-7(MicroRNA-7,miRNA-7)對小鼠腸系膜淋巴結(jié)αβT淋巴細(xì)胞比例的影響并初步探討其意義。方法:利用miR-7基因敲減(Knockdown,KD)小鼠模型,觀察其腸系膜淋巴結(jié)大小、重量和淋巴結(jié)細(xì)胞總數(shù),以及HE染色的病理學(xué)變化;流式細(xì)胞術(shù)(FACS)檢測淋巴結(jié)中αβT淋巴細(xì)胞亞群(CD3+T細(xì)胞、CD4+T細(xì)胞和CD8+T細(xì)胞)以及CD4+T細(xì)胞和CD8+T細(xì)胞的活化相關(guān)分子CD69、CD62L和功能相關(guān)因子IFN-γ表達(dá)的情況。結(jié)果:與野生型(Wild type,WT)小鼠相比,miR-7KD小鼠腸系膜淋巴結(jié)大小、重量指數(shù)和淋巴結(jié)細(xì)胞總數(shù)均顯著增高(P0.05),且HE染色顯示MLNs有病理性改變;腸系膜淋巴結(jié)中αβCD3+T細(xì)胞比例增加顯著,其中以CD4+T細(xì)胞比例變化最為明顯(P0.05),而CD19+B淋巴細(xì)胞比例明顯下調(diào)(P0.05),但各亞群細(xì)胞總數(shù)均明顯增加(P0.05);最后,CD4+T細(xì)胞和CD8+T細(xì)胞中的CD62L+細(xì)胞的比例均顯著下調(diào)(P0.05),而CD69+細(xì)胞和IFN-γ+細(xì)胞比例均顯著上調(diào)(P0.05)。結(jié)論:miR-7敲減后可顯著影響腸系膜淋巴結(jié)中αβT淋巴細(xì)胞的組成比例,提示其對腸系膜淋巴結(jié)中淋巴細(xì)胞的組成和功能具有重要的調(diào)控作用。
[Abstract]:Objective: to study the effect of miR-7 microRNA-7 miRNA-7 on the percentage of 偽 尾 T lymphocytes in mesenteric lymph nodes of mice and its significance. Methods: the size, weight and total number of mesenteric lymph nodes were observed by using miR-7 gene knockdown KD- mouse model. And pathological changes of HE staining; Flow cytometry (FACS) was used to detect the expression of activation molecules CD69, CD62L and IFN- 緯 in 偽 尾 T lymphocyte subsets (CD 4 T cells and CD8 T cells), CD4 T cells and CD8 T cells in lymph nodes. The mesenteric lymph nodes of wild type WT-mice were larger than those of miR-7KD mice. The weight index and the total number of lymph node cells were significantly increased, and HE staining showed that MLNs had pathological changes, and the percentage of 偽 尾 CD3 T cells in mesenteric lymph nodes increased significantly, and the percentage of 偽 尾 CD3 T cells in mesenteric lymph nodes increased significantly. The percentage of CD4 T cells was the most obvious, while the proportion of CD19 B lymphocytes was down-regulated, but the total number of cells in each subgroup increased significantly. Finally, the percentage of CD62L cells in CD4 T cells and CD8 T cells decreased significantly, while the percentage of CD62L cells in CD8 T cells decreased significantly. The proportion of CD69 cells and IFN- 緯 cells were significantly up-regulated (P 0.05). Conclusion the proportion of 偽 尾 T lymphocytes in mesenteric lymph nodes can be significantly affected by the knockout of miR-7. The results suggest that it plays an important role in regulating the composition and function of lymphocytes in mesenteric lymph nodes.
【作者單位】： 遵義醫(yī)學(xué)院免疫學(xué)教研室暨貴州省免疫學(xué)研究生教育創(chuàng)新基地;遵義醫(yī)學(xué)院醫(yī)學(xué)物理學(xué)教研室;
【基金】：國家自然科學(xué)基金(No.31370918) 教育部“新世紀(jì)優(yōu)秀人才計劃”項(xiàng)目(NCET-12-0661)資助
【分類號】：R392

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7 陳鑫s，

本文編號：1590852