本文關鍵詞： 腎囊阻滯 胃內殘留率 小腸推進率 胃動素 一氧化氮　出處：《中南大學》2010年碩士論文　論文類型：學位論文

【摘要】： 目的：以胃腸動力異常的大鼠為研究對象,觀察腎囊阻滯對胃內殘留率、小腸推進率、腦腸肽激素以及腸神經(jīng)遞質的變化。探討腎囊阻滯調節(jié)胃腸動力的規(guī)律及機制。 方法： 1、將72只雄性SD大鼠隨機均分為3大組,組A為非模型組,組B為腹腔注射阿托品制作的大鼠胃腸運動抑制模型,組C為腹腔注射新斯的明制作的大鼠胃腸運動亢進模型。每大組又隨機均分為3小組,每小組8只大鼠,組A1、B1、C1為假手術組,即僅暴露腎臟及腎囊,組A2、B2、C2為腎囊注射生理鹽水組,組A3、B3、C3為腎囊注射利多卡因組。 2、各組大鼠經(jīng)腎囊阻滯后,予以半固體食物灌胃。通過稱胃全重及胃凈重計算胃內殘留率,并用炭末法測量小腸推進率,觀察各組大鼠胃內殘留率、小腸推進率的變化。 3、用放射免疫法檢測胃竇、下丘腦組織中腦腸肽胃動素(MTL)的含量,觀察各組大鼠MTL的變化。 4、用硝酸還原酶法檢測結腸組織中神經(jīng)遞質一氧化氮(NO)的含量,觀察各組大鼠NO的變化。 結果： 1、與組A1相比,組Bl的胃內殘留率顯著升高,小腸推進率、胃竇和下丘腦中MTL含量顯著降低(p0.05),組C1的胃內殘留率顯著降低,小腸推進率、胃竇和下丘腦中MTL含量顯著升高(p0.05)。 2、組A1與組A2、組B1與組B2以及組C1與組C2相比,胃內殘留率、小腸推進率以及胃竇和下丘腦中MTL含量無顯著性差異(p0.05)。 3、在非模型組中,組A1與組A3相比,胃竇MLT含量無顯著性差異(p0.05),組A3的胃內殘留率顯著降低,小腸推進率、下丘腦MTL的含量顯著增高(p0.05),組A2與組A3相比,胃內殘留率、胃竇和下丘腦MLT含量無顯著差異(p0.05),組A3的小腸推進率顯著升高(p0.05)；在抑制模型組中,與組B1和組B2比較,組B3的胃內殘留率顯著降低,小腸推進率、胃竇和下丘腦MTL含量顯著增高(p0.05)；在亢進模型組中,與組C1和組C2比較,組C3的胃內殘留率顯著增高,小腸推進率、胃竇和下丘腦MTL含量顯著降低(p0.05)。 4、各組大鼠結腸NO含量無顯著性差異(p0.05)。 結論： 1、腎囊阻滯可能通過升高胃腸動力低下動物模型體內MTL含量,加快阿托品抑制模型的胃排空、小腸推進,也可降低胃腸動力亢進動物模型體內MTL含量,減慢新斯的明亢進模型的胃排空、小腸推進,提示腎囊阻滯可通過神經(jīng)體液調節(jié)對胃腸運動起到雙向調節(jié)作用。 2、腎囊阻滯對胃腸道正常狀態(tài)氮能神經(jīng)NO含量無明顯影響。
[Abstract]:Aim: to observe the changes of residual rate in stomach, small intestine propulsion rate, brain intestinal peptide hormone and intestinal neurotransmitter in rats with abnormal gastrointestinal motility, and to investigate the regulation and mechanism of renal capsule block on gastrointestinal motility. Methods:. 1. 72 male Sprague-Dawley rats were randomly divided into 3 groups. Group A was a non-model group and group B was a gastrointestinal motility inhibition model made by intraperitoneal injection of atropine. Group C was induced by intraperitoneal injection of neostigmine. Each group was randomly divided into 3 groups (8 rats in each group). Group A _ (1) B _ (1) C _ (1) was sham-operated group, that is, only kidney and renal sac were exposed, group A _ (2) B _ (2) C _ (2) was injected normal saline into renal capsule, and group A _ (2) B _ (2) C _ (2) was injected with normal saline. Group A _ 3 B _ 3 C _ 3 was injected with lidocaine into renal capsule. 2. Rats in each group were fed with semi-solid food after renal capsule block. The gastric residual rate was calculated by weighing the whole stomach weight and the net gastric weight, and the small intestinal propulsion rate was measured by carbon powder method. The changes of gastric residual rate and small intestinal propulsion rate were observed in each group. 3. The contents of motilin motilin in antrum and hypothalamus were detected by radioimmunoassay, and the changes of MTL were observed in each group. 4. Nitrate reductase method was used to detect the content of nitric oxide (no) in colon tissue, and the changes of no in each group were observed. Results:. 1. Compared with group A1, the gastric residual rate of group B1 was significantly increased, the intestinal propulsion rate, the MTL content in gastric antrum and hypothalamus was significantly decreased, the gastric residual rate in group C1 was significantly decreased, the intestinal propulsive rate and MTL content in antrum and hypothalamus were significantly increased (P 0.05). 2There was no significant difference between group A1 and group A2, group B1 with group B2 and group C1 with group C2 in gastric residual rate, intestinal propulsion rate and MTL content in antrum and hypothalamus (p0.05). 3. In the non-model group, there was no significant difference in gastric antral MLT content between group A1 and group A3 (p0.05). The gastric residual rate of group A3 was significantly lower, the rate of small intestinal propulsion and the content of MTL in hypothalamus was significantly higher than that of group A _ 2 and group A _ 3, and the intragastric residual rate of group A _ 2 was significantly higher than that of group A _ 3. There was no significant difference in MLT content between antrum and hypothalamus (p0.05), but the small intestinal propulsive rate of group A _ 3 was significantly higher than that of group B _ 1 and B _ 2.Compared with group B _ 1 and group B _ 2, the gastric residual rate of group B _ 3 was significantly lower than that of group B _ 2, and the rate of small intestinal propulsion was significantly lower in group A _ 3. The contents of MTL in antrum and hypothalamus were significantly increased in the hyperactive model group compared with group C1 and group C2, the gastric residual rate of group C _ 3 was significantly higher, the rate of small intestinal propulsion and the content of MTL in antrum and hypothalamus were significantly lower than those in group C _ 1 and group C _ 2, and the contents of MTL in antrum and hypothalamus were significantly lower than those in group C _ 2. 4, there was no significant difference in no content in colon of rats in each group (P 0.05). Conclusion:. 1. Renal sac block may increase the MTL content in the gastrointestinal hypokinetic animal model, accelerate the gastric emptying and intestinal propulsion of the atropine inhibition model, and decrease the MTL content in the hypergastrointestinal hyperkinetic animal model. The decrease of gastric emptying and small intestinal propulsion in neostigmine model suggests that renal capsule block can regulate gastrointestinal motility through neurohumoral regulation. 2. Renal capsule block had no effect on no content in normal gastrointestinal tract.
【學位授予單位】：中南大學
【學位級別】：碩士
【學位授予年份】：2010
【分類號】：R333

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