本文關(guān)鍵詞： 飲食限制 細(xì)胞自噬 生存分析 氧化應(yīng)激 抗衰老　出處：《山西醫(yī)科大學(xué)》2010年碩士論文　論文類型：學(xué)位論文

【摘要】： 目的：觀察不同飲食方式下雌性老齡大鼠發(fā)生細(xì)胞自噬的差異,同時(shí)研究飲食限制對(duì)雌性老齡大鼠血清超氧化物歧化酶(superoxide dismutase,SOD)活性和丙二醛(malondialdehyde, MDA)含量的影響,分析實(shí)驗(yàn)動(dòng)物的自然壽命及其生存曲線,探討飲食限制抗衰老的生物學(xué)效應(yīng)及機(jī)制。 方法：45只24月齡雌性健康SD大鼠隨機(jī)、平均分成三組：對(duì)照組(正常飲食組)、隔日禁食組(一日禁食一日進(jìn)食組)和兩日禁食組(持續(xù)禁食兩日,持續(xù)進(jìn)食五日組)。不同飲食方式干預(yù)三月后采集標(biāo)本。透射電鏡觀察肝臟細(xì)胞自噬泡并對(duì)細(xì)胞的自噬活性進(jìn)行定量分析；試劑盒測(cè)定大鼠血清SOD活性和MDA含量；繪制生存曲線,進(jìn)行生存分析；對(duì)細(xì)胞自噬活性和大鼠生存時(shí)間進(jìn)行關(guān)聯(lián)性分析；對(duì)血清SOD活性、MDA含量和大鼠生存時(shí)間進(jìn)行關(guān)聯(lián)性分析。 結(jié)果：與對(duì)照組比較,隔日禁食組和兩日禁食組大鼠肝臟細(xì)胞自噬泡占胞漿總面積的比值增高,差異均有統(tǒng)計(jì)學(xué)意義(P0.01),而此兩組間差別無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。與對(duì)照組比較,隔日禁食組SOD活性增高(P0.01),MDA含量降低(P0.01)；兩日禁食組SOD活性增高(P0.05),MDA含量降低(P0.01),而此兩組間SOD活性及MDA含量差別均無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。生存分析結(jié)果顯示三組生存曲線不同,與對(duì)照組比較,隔日禁食組和兩日禁食組生存率增高,差異均有統(tǒng)計(jì)學(xué)意義(P0.01),而此兩組間差別無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。對(duì)細(xì)胞自噬活性和大鼠生存時(shí)間進(jìn)行關(guān)聯(lián)性分析,結(jié)果為rS=0.903,表明細(xì)胞自噬活性和大鼠生存時(shí)間之間呈線性正相關(guān)。對(duì)血清SOD活性和大鼠生存時(shí)間進(jìn)行關(guān)聯(lián)性分析,結(jié)果為rS=0.717,同時(shí)對(duì)MDA含量和大鼠生存時(shí)間進(jìn)行關(guān)聯(lián)性分析,結(jié)果為rS=—0.745,表明SOD活性和大鼠生存時(shí)間之間呈線性正相關(guān),而MDA含量和大鼠生存時(shí)間之間呈線性負(fù)相關(guān)。 結(jié)論：1細(xì)胞自噬呈高活性狀態(tài)有利于大鼠壽命的延長(zhǎng)；飲食限制導(dǎo)致的壽命延長(zhǎng)與細(xì)胞自噬相關(guān)。2對(duì)老年大鼠進(jìn)行飲食限制,能明顯提高SOD活性,降低MDA含量,改善氧化應(yīng)激水平。3飲食限制通過誘導(dǎo)細(xì)胞自噬、改善氧化應(yīng)激等生物學(xué)機(jī)制發(fā)揮其抗衰老效應(yīng)。
[Abstract]:Objective: to observe the difference of autophagy in aged female rats under different dietary patterns, and to study the effects of dietary restriction on the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDAs) in serum of aged female rats. The natural life span and survival curve of experimental animals were analyzed, and the biological effects and mechanism of dietary restriction on anti-aging were discussed. Methods 45 healthy female SD rats aged 24 months were randomly divided into three groups: control group (normal diet group), fasting group (1st fasting 1st group) and two day fasting group (two days fasting for two days), the control group (normal diet group), the second day fasting group (1st fasting 1st group) and the two day fasting group (two days). The liver cells were observed by transmission electron microscope (TEM) and the autophagy activity was quantitatively analyzed. The serum SOD activity and MDA content were measured by kit, and the survival curve was drawn. The relationship between autophagy activity and survival time of rats was analyzed, and the relationship between serum SOD activity and survival time of rats was analyzed. Results: compared with the control group, the ratio of hepatic autophagy to total cytosolic area in the two groups was significantly higher than that in the control group, but there was no significant difference between the two groups. The activity of SOD was increased in the fasting group on the next day and the content of SOD was decreased in the fasting group on the following day. The activity of SOD in the two groups was increased and the content of MDA was decreased in the two groups. There was no significant difference in the activity of SOD and the content of MDA between the two groups. The survival analysis showed that the survival curves of the three groups were different, the results of survival analysis showed that the survival curves of the three groups were different. Compared with the control group, the survival rate of the two groups was significantly higher than that of the control group (P 0.01), but there was no significant difference between the two groups. The relationship between the cell autophagy activity and the survival time of rats was analyzed. The results showed that there was a linear positive correlation between autophagy activity and survival time of rats. The correlation between serum SOD activity and survival time of rats was analyzed. The results were as follows: rS0. 717. The correlation between the content of MDA and the survival time of rats was also analyzed. The results showed that there was a positive linear correlation between the activity of SOD and the survival time of rats, but a negative correlation between the content of MDA and the survival time of rats. Conclusion the hyperactive state of autophagy of 1 / 1 cell is beneficial to the prolongation of the life span of rats, and the prolongation of life span caused by dietary restriction and the dietary restriction on the aged rats induced by dietary restriction can obviously increase the activity of SOD and decrease the content of MDA. Improving oxidative stress level 3. 3 dietary restriction exerts its anti-aging effect by inducing autophagy and improving oxidative stress and other biological mechanisms.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：R339.38

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 高嘉彬;羅亮;黃昶荃;;老齡2型糖尿病大鼠維生素D與胰島素抵抗的關(guān)系[J];中國(guó)老年學(xué)雜志;2011年17期

2 程s，

本文編號(hào)：1497960