本文關(guān)鍵詞：TGF-β1、bFGF在胎兒角膜表達(dá)的免疫組化研究　出處：《山東大學(xué)》2008年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 胎兒 角膜 轉(zhuǎn)化生長(zhǎng)因子-β1 堿性成纖維細(xì)胞生長(zhǎng)因子

【摘要】： [目的]研究轉(zhuǎn)化生長(zhǎng)因子-β1(transforming growth factor-β1,TGF-β1)和堿性成纖維細(xì)胞生長(zhǎng)因子(basic fibroblast growth factor,bFGF)在不同胎齡胎兒角膜的表達(dá)及兩種因子間表達(dá)的差異,了解TGF-β1和bFGF在正常角膜胚胎發(fā)育過程中的作用及變化規(guī)律,為角膜的胚胎發(fā)育提供相關(guān)資料。 [方法]收集11～20周各種方法引產(chǎn)的胎兒23例共46只眼,采用免疫組織化學(xué)鏈酶親和素—生物素—過氧化物酶復(fù)合物(streptavdin-biotin-complex,SABC)的方法,檢測(cè)各胎齡胎兒角膜中TGF-β1和bFGF的表達(dá),并分析TGF-β1與bFGF的相關(guān)性。 [結(jié)果]1、TGF-β1和bFGF在各胎齡角膜上皮層均有表達(dá)。 2、TGF-β1和bFGF在各胎齡角膜其它層次內(nèi)均未見表達(dá)。 3、TGF-β1和bFGF在角膜上皮層的表達(dá)均隨胎齡的增加而減少,兩者比較差異具有統(tǒng)計(jì)學(xué)意義(P＜0.05)。 4、相同胎齡胎兒角膜上皮層內(nèi)TGF-β1與bFGF的表達(dá)有顯著性差異,TGF-β1的表達(dá)水平高于bFGF的表達(dá)水平,兩者比較差異具有統(tǒng)計(jì)學(xué)意義(P＜0.05)。 5、經(jīng)Pearson相關(guān)性分析:TGF-β1與bFGF呈正相關(guān)(r=0.8037),具有統(tǒng)計(jì)學(xué)意義(P＜0.05)。 [結(jié)論]1、各個(gè)胎齡的胎兒角膜上皮層均有TGF-β1和bFGF的表達(dá),說明二者均在胎兒角膜上皮發(fā)育中發(fā)揮一定的作用。 2、各個(gè)胎齡的胎兒角膜其它層次內(nèi)未見TGF-β1和bFGF的表達(dá),具體機(jī)制不清。 3、TGF-β1和bFGF在胎兒角膜上皮層的表達(dá)均隨胎齡的增加而減少。 4、相同胎齡胎兒角膜上皮層內(nèi)TGF-β1的表達(dá)高于bFGF的表達(dá)。 5、TGF-β1和bFGF呈正相關(guān),說明TGF-β1和bFGF在胎兒角膜上皮的發(fā)育中有協(xié)同作用。
[Abstract]:[Objective] Study on transforming growth factor beta 1 (transforming growth factor- TGF- beta 1, beta 1) and basic fibroblast growth factor (basic fibroblast, growth factor, bFGF) differential expression expression in fetal cornea and two kinds of factors, to understand the process in normal cornea and the role in embryonic development the regularity of TGF- beta 1 and bFGF, to provide relevant information for corneal embryo development.
[Methods] from 11 to 20 weeks of induction of various methods of fetal 23 cases of 46 eyes, using immunohistochemical streptavidin - biotin - peroxidase complex (streptavdin-biotin-complex, SABC) method, the expression of the fetal cornea in TGF- beta 1 and bFGF, and correlation analysis of TGF- beta 1 with bFGF.
[results]1, TGF- beta 1 and bFGF were expressed in the corneal epithelium at all fetal age.
2, TGF- beta 1 and bFGF were not expressed in the other layers of the cornea of all gestational ages.
3, the expression of TGF- beta 1 and bFGF in the corneal epithelium decreased with the increase of fetal age, and the difference was statistically significant (P < 0.05).
4, there was a significant difference in the expression of TGF- beta 1 and bFGF in the corneal epithelium of the same gestational age. The expression level of TGF- beta 1 was higher than that of bFGF, and the difference between them was statistically significant (P < 0.05).
5, after Pearson correlation analysis, TGF- beta 1 was positively correlated with bFGF (r=0.8037), and was statistically significant (P < 0.05).
[conclusion]1, the expression of TGF- beta 1 and bFGF in all fetal corneal epithelium at all fetal age shows that all two of them play a role in the development of fetal corneal epithelium.
2, there is no expression of TGF- beta 1 and bFGF in the other layers of fetal cornea at all fetal age, and the specific mechanism is not clear.
3, the expression of TGF- beta 1 and bFGF in the epithelial layer of fetal cornea decreased with the increase of fetal age.
4, the expression of TGF- beta 1 in the corneal epithelium of the fetus of the same gestational age was higher than that of bFGF.
5, there is a positive correlation between TGF- beta 1 and bFGF, indicating that TGF- beta 1 and bFGF have synergistic effects in the development of fetal corneal epithelium.

【學(xué)位授予單位】：山東大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2008
【分類號(hào)】：R772;R321.5

【引證文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前1條

1 王志軍;TGF-β1/Smad4在氧誘導(dǎo)小鼠視網(wǎng)膜病變中的作用研究[D];暨南大學(xué);2010年

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本文編號(hào)：1379803