【摘要】： 目的: 探討IL-2預(yù)孵育對G-CSF動員后的小鼠脾淋巴細(xì)胞功能的影響,從而為減輕異基因造血干細(xì)胞移植中急性GVHD提供一種新的方法 方法: 1,予rhG-CSF(0.2μg/g.d)動員的C57BL/6小鼠脾細(xì)胞和C57BL/6小鼠na(1|¨)veCD4+T細(xì)胞,BALB/c小鼠異基因抗原分別對其進(jìn)行刺激,測定C57BL/6小鼠脾細(xì)胞和C57BL/6小鼠na(1|¨)veCD4+T細(xì)胞增殖能力和分化方向 2,用IL-2(50U/mL)預(yù)孵育C57BL/6小鼠脾細(xì)胞,檢測脾細(xì)胞SOCS-3基因的表達(dá)變化 3,用IL-2(50U/mL)預(yù)孵育C57BL/6小鼠脾細(xì)胞和C57BL/6小鼠na(1|¨)veCD4+T細(xì)胞,然后BALB/c小鼠異基因抗原分別對其進(jìn)行刺激,測定C57BL/6小鼠脾細(xì)胞和C57BL/6小鼠na(1|¨)veCD4+T細(xì)胞免疫增殖能力和分化方向 4,IL-2(50U/mL)預(yù)孵育經(jīng)rhG-CSF(0.25μg/g.d)動員的C57BL/6小鼠脾細(xì)胞和C57BL/6小鼠na(1|¨)veCD4+T細(xì)胞,然后BALB/c小鼠異基因抗原分別對其進(jìn)行刺激,測定動員后的C57BL/6小鼠脾細(xì)胞和C57BL/6小鼠na(1|¨)veCD4+T細(xì)胞免疫增殖能力和分化方向 結(jié)果: 1,經(jīng)過一定濃度rhG-CSF動員的C57BL/6小鼠脾細(xì)胞和C57BL/6小鼠na(1|¨)veCD4+T細(xì)胞對同種異基因抗原免疫增殖能力減弱,并向Th2方向分化 2,C57BL/6小鼠脾細(xì)胞經(jīng)IL-2預(yù)孵育后,SOCS-3的表達(dá)升高,在6h達(dá)到高峰 3,C57BL/6小鼠脾細(xì)胞和C57BL/6小鼠na(1|¨)veCD4+T細(xì)胞經(jīng)IL-2預(yù)孵育后對同種異基因抗原免疫增殖能力明顯減弱并向Th2方向分化 4,rhG-CSF動員的C57BL/6小鼠脾細(xì)胞和C57BL/6小鼠na(1|¨)veCD4+T細(xì)胞經(jīng)IL-2預(yù)孵育后對同種異基因抗原免疫增殖能力明顯減弱并向Th2方向分化,兩者合用在誘導(dǎo)T淋巴細(xì)胞免疫耐受上有協(xié)同效應(yīng)。
[Abstract]:Objective: to investigate the effect of IL-2 pre-incubation on the function of spleen lymphocytes in mice mobilized with G-CSF, so as to provide a new method for reducing acute GVHD in allogenic hematopoietic stem cell transplantation: 1, spleen cells of C57BL/6 mice mobilized by rhG-CSF (0.2 渭 g / g 路d) and na (1) veCD4 T cells of C57BL/6 mice. The spleen cells of BALB/c mice were stimulated by allogenic antigen. The proliferation and differentiation direction of na (1) veCD4 T cells in C57BL/6 mice and C57BL/6 mice were measured. The spleen cells of C57BL/6 mice were preincubated with IL-2 (50U/mL), and the expression of SOCS-3 gene in spleen cells was detected. Spleen cells of C57BL/6 mice and na (1) veCD4 T cells of C57BL/6 mice were preincubated with IL-2 (50U/mL), and then stimulated by allogenic antigen of BALB/c mice. The immune proliferation ability and differentiation direction of spleen cells of C57BL/6 mice and C57BL/6 mice na (1 / 1) veCD4 T cells were measured. IL-2 (50U/mL) preincubated C57BL/6 mouse spleen cells and C57BL/6 mouse na (1) veCD4 T cells mobilized by rhG-CSF (0.25 渭 g / g 路d), and then BALB/c mouse allogenic antigens stimulated them respectively. the immune proliferation ability and differentiation direction of mobilized C57BL/6 mouse spleen cells and C57BL/6 mouse na (1) veCD4 T cells were measured. The spleen cells of C57BL/6 mice mobilized by a certain concentration of rhG-CSF and na (1) veCD4 T cells of C57BL/6 mice attenuated the immune proliferation ability of allogenic antigen and differentiated into Th2 direction 2. The expression of SOCS-3 increased after preincubated with IL-2 and reached the peak of 3 at 6 h. Spleen cells of C57BL/6 mice and na (1) veCD4 T cells of C57BL/6 mice were preincubated with IL-2 and their immune proliferation ability to allogenic antigen was significantly weakened and differentiated into Th2 direction. Spleen cells of C57BL/6 mice mobilized by RHG and na (1) veCD4 T cells of C57BL/6 mice were significantly attenuated and differentiated into Th2 direction after IL-2 preincubation. The combination of the two had synergistic effect on inducing immune tolerance of T lymphocytes.
【學(xué)位授予單位】：復(fù)旦大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2009
【分類號】：R392

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