【摘要】：體外BMSCs分化為心肌細胞過程中,細胞在形態(tài)及相關(guān)基因的表達上發(fā)生明顯的變化。本研究以心肌組織裂解液誘導(dǎo)BMSCs,觀察分化過程中細胞形態(tài)變化,根據(jù)心肌細胞發(fā)生過程中細胞形態(tài)變化規(guī)律,選定5個檢測點,行PCR檢測細胞中Nkx2.5的表達,以探究分化過程中細胞形態(tài)變化與相關(guān)基因表達規(guī)律及其之間的相關(guān)性。實驗結(jié)果表明BMSCs分化為心肌細胞的過程中,細胞形態(tài)變化呈現(xiàn)明顯規(guī)律:由單個大核,胞漿飽滿,折光性好的紡錘形;逐漸演變?yōu)槎虠U狀;形成突起并連接成網(wǎng)狀結(jié)構(gòu);細胞端相接呈"竹節(jié)樣"改變;最后形成肌管樣結(jié)構(gòu);誘導(dǎo)前細胞不表達Nkx2.5,誘導(dǎo)過程中隨著細胞形態(tài)的不斷變化Nkx2.5持續(xù)表達并逐漸增強。說明間充質(zhì)干細胞分化為心肌細胞的過程中,Nkx2.5表達相對量的變化與細胞的形態(tài)變化可能有一定的相關(guān)。
[Abstract]:During the differentiation of BMSCs into cardiomyocytes in vitro, there were obvious changes in morphology and expression of related genes. In this study, BMSCs, induced by myocardial lysate was used to observe the changes of cell morphology during differentiation. According to the changes of cell morphology during cardiomyogenesis, five detection points were selected and the expression of Nkx2.5 in the cells was detected by PCR. The purpose of this study was to explore the relationship between the morphological changes of cells and the expression of related genes in the process of differentiation. The results showed that during the process of differentiation of BMSCs into cardiomyocytes, the morphological changes of the cells showed obvious regularity: from a single nucleus, full cytoplasm and good refractive spindle, gradually evolved into short rod, formed protuberance and joined into reticular structure, and developed into a short rod, formed a protuberance and joined together into a reticular structure. The terminal phase of the cells was changed as "bamboo node-like", and finally the myotubule-like structure was formed. During the induction process, the cells did not express Nkx2.5, and the Nkx2.5 continued to express and gradually increased with the continuous changes of the cell morphology. These results suggest that the relative amount of Nkx2.5 expression may be related to the morphological changes of MSCs in the process of differentiation into cardiomyocytes.
【作者單位】： 廣西醫(yī)科大學(xué)組織學(xué)與胚胎學(xué)教研室;
【基金】：廣西自然科學(xué)基金(2012GXNSFAA053126) 廣西科技廳資助項目(桂科自0832157)、廣西科技廳資助項目(桂科攻0592007-1G)、廣西科技廳資助項目(桂科青0229009)共同資助
【分類號】：R329.2

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