【摘要】： 細胞間黏附分子-1(ICAM-1)作為淋巴細胞功能相關(guān)抗原-1 (LFA-1)的配體可介導白細胞與血管內(nèi)皮細胞的黏附及白細胞穿出血管壁,同時促使更多中性粒細胞和嗜酸性粒細胞轉(zhuǎn)移至炎癥區(qū),釋放更多的氧化自由基、蛋白酶和花生四烯酸等代謝產(chǎn)物,加重組織損傷程度,進而造成病理損傷,在炎癥性疾病的發(fā)生與發(fā)展過程中發(fā)揮重要作用,ICAM-1成為炎癥相關(guān)性疾病治療新的靶點。 本研究旨在利用噬菌體展示技術(shù)應用Tomlinson I+J噬菌體抗體庫制備人源抗ICAM-1單鏈抗體,并對工程菌進行表達條件優(yōu)化、目的蛋白純化工藝研究及生物學活性研究。人血管內(nèi)皮細胞-單核細胞黏附抑制實驗表明,制備的人源抗ICAM-1單鏈抗體能夠有效抑制血管內(nèi)皮細胞與單核細胞之間的黏附,對炎癥性病理反應有較強的抑制作用,本課題研究為臨床炎癥相關(guān)性疾病的初步治療奠定基礎(chǔ)。
[Abstract]:Intercellular adhesion molecule-1 (ICAM-1), as a ligand of lymphocyte function-related antigen-1 (LFA-1), mediates leukocyte adhesion to vascular endothelial cells and leukocytes through the vascular wall. At the same time, it causes more neutrophils and eosinophils to transfer to the inflammatory zone and releases more metabolites such as oxidative free radicals, protease and arachidonic acid, which aggravates the degree of tissue damage and then causes pathological damage. ICAM-1 plays an important role in the occurrence and development of inflammatory diseases, and it has become a new target for the treatment of inflammatory-related diseases. The aim of this study was to prepare human anti-ICAM-1 scFv by phage display technique using Tomlinson I J phage antibody library, and to optimize the expression conditions of the recombinant strain, and to study the purification technology and biological activity of the protein. Human vascular endothelial cell-monocyte adhesion inhibition test showed that the prepared human anti-ICAM-1 single chain antibody could effectively inhibit the adhesion between vascular endothelial cells and monocytes, and had a strong inhibitory effect on inflammatory pathological response. This study lays a foundation for the preliminary treatment of clinical inflammation-related diseases.
【學位授予單位】：吉林大學
【學位級別】：碩士
【學位授予年份】：2009
【分類號】：R392.12

【相似文獻】

相關(guān)期刊論文 前10條

1 許建英,杜永成,徐永健;吸煙的慢性支氣管炎患者肺泡巨噬細胞核因子-κB和細胞間黏附分子-1的表達[J];中華內(nèi)科雜志;2003年05期

2 祁s，

本文編號：2460219