【摘要】：目的依牧民飲茶習(xí)慣建立大鼠慢性磚茶型氟鋁聯(lián)合中毒和低氟低鋁磚茶模型,觀察分析磚茶中氟鋁對(duì)大鼠體重、學(xué)習(xí)記憶、神經(jīng)系統(tǒng)、血液系統(tǒng)、骨骼系統(tǒng)的影響及各組織的氟鋁含量,探討磚茶型氟鋁聯(lián)合中毒的病理生理機(jī)制。為根治磚茶型氟鋁聯(lián)合中毒提供理論依據(jù)。方法離乳4周的雄性Wistar大鼠24只、雌性36只,隨機(jī)分為6組�？瞻讓�(duì)照組(飲自來水)、單鋁組(飲水含鋁159mg/L)、單氟組(飲水含氟100mg/L)、氟鋁聯(lián)合組(飲水含鋁159mg/L、含氟100mg/L)、磚茶組(飲磚茶水)、低氟普洱茶組(飲低氟普洱茶水)。各組皆以配制的飲品為飲水唯一來源,自由飲食,實(shí)驗(yàn)中每三個(gè)月測(cè)大鼠體重；實(shí)驗(yàn)最后兩個(gè)月對(duì)其學(xué)習(xí)記憶行為評(píng)價(jià)；實(shí)驗(yàn)結(jié)束(一年)后處死,測(cè)其腦組織中生長(zhǎng)抑素含量；觀察腦、肝、脾、腎的微觀結(jié)構(gòu)；并測(cè)血常規(guī)及血清、腎、肝、骨、牙、腦中的氟、鋁含量。實(shí)驗(yàn)所得數(shù)據(jù)以均數(shù)±標(biāo)準(zhǔn)差(X±S)表示,用SPSS13.0軟件進(jìn)行統(tǒng)計(jì)學(xué)分析處理,檢驗(yàn)水準(zhǔn)取α=0.05。結(jié)果鋁可增加大鼠體重(p0.05)；氟鋁聯(lián)合比單鋁的作用慢但持久且嚴(yán)重(p0.05)；低氟普洱茶能使一年齡大鼠體重下降(p0.05)。鋁引起學(xué)習(xí)記憶功能障礙(p0.05)；氟鋁聯(lián)合比單鋁的作用嚴(yán)重(p0.01)；磚茶亦引起學(xué)習(xí)記憶功能障礙(p0.05)。氟、鋁都引起大鼠海馬SS數(shù)量下降(p0.05)；氟鋁聯(lián)合比單鋁作用嚴(yán)重(p0.01)；磚茶比單氟作用嚴(yán)重(p0.01)。高濃度氟能提高大鼠的MCV、PLT(p0.05);低氟普洱茶能降低大鼠HCT、MCV、MCHC(P0.05)。高濃度鋁可促進(jìn)氟吸收并在血清、骨、牙、腦、肝中蓄積；高濃度鋁可蓄積在血清、骨、牙、腦中(p0.05),高濃度氟可抑制鋁吸收。磚茶引起骨、牙、肝氟含量增加(p0.05)及牙、肝鋁含量增加(p0.05)；低氟普洱茶引起腎、肝鋁含量增加(p0.05)。氟鋁聯(lián)合和單鋁組大鼠腦組織出現(xiàn)腦水腫；氟鋁聯(lián)合和單氟組腎出現(xiàn)蛋白尿；氟鋁聯(lián)合和磚茶組肝出現(xiàn)小面積的點(diǎn)狀壞死灶。結(jié)論1、成功復(fù)制了磚茶型氟鋁聯(lián)合中毒大鼠動(dòng)物模型。2、氟元素針對(duì)的靶器官是骨和牙,并能引起相應(yīng)的損傷。鋁元素針對(duì)的靶器官是骨、牙及腦,能引起生長(zhǎng)發(fā)育障礙及學(xué)習(xí)記憶功能障礙。3、氟鋁同時(shí)存在時(shí),高濃度鋁可促進(jìn)氟吸收,高濃度氟可抑制鋁吸收。4、選用低氟普洱茶代替普通磚茶,可在不影響磚茶口味和色澤的前提下,預(yù)防磚茶型氟鋁聯(lián)合中毒的發(fā)牛
[Abstract]:Objective to establish chronic brick tea poisoning and low fluoride and low aluminum brick tea models in rats according to herdsman's tea drinking habits, and to observe and analyze the effects of fluoride and aluminum in brick tea on rats' weight, learning and memory, nervous system and blood system. The effects of skeletal system and the content of fluoride and aluminum in tissues were studied to explore the pathophysiological mechanism of brick-tea fluoro-aluminum poisoning. It provides a theoretical basis for the radical cure of combined fluoro-aluminum poisoning in brick tea. Methods 24 male Wistar rats and 36 female rats were randomly divided into 6 groups. Blank control group (drinking tap water), single aluminum group (drinking water containing aluminum 159mg/L), single fluoride group (drinking water containing fluoride 100mg/L), fluoride and aluminum combination group (drinking water containing aluminum 159 mg / L, fluoride containing 100mg/L), brick tea group (drinking brick tea water), Low fluoride Pu'er tea group (drinking low fluorine Pu'er tea water). The rats' weight was measured every three months, and the learning and memory behaviors of rats were evaluated at the last two months of the experiment, and the rats in each group were treated with the drink as the only source of drinking water, and the rats were given a free diet at the last two months. The contents of somatostatin in brain tissue, microstructure of brain, liver, spleen and kidney, blood routine test, serum, kidney, liver, bone, tooth and brain were measured. The experimental data were expressed as mean 鹵standard deviation (X 鹵S) and analyzed by SPSS13.0 software. The level was 偽 = 0.05. Results Aluminum could increase the body weight of rats (p0.05), the effect of fluoride-aluminum combination was slower but longer and more serious than that of single aluminum (p0.05), and low-fluorine Pu-erh tea could decrease the weight of one-year-old rats (p0.05). Aluminum induced learning and memory dysfunction (p0.05), fluoride combined with aluminum had more effect than aluminum (p0.01), brick tea also caused learning and memory dysfunction (p0.05). Fluoride and aluminum could decrease the number of SS in hippocampus of rats (p0.05), the effect of fluoride combined with aluminum was more serious than that of single aluminum (p0.01), and the effect of brick tea was more serious than that of monofluoride (p0.01). High concentration of fluoride could increase the MCV,PLT of rats (p0.05), and low fluoride Pu'er tea could decrease the HCT,MCV,MCHC of rats (P0.05). High concentration of aluminum can promote fluoride absorption and accumulate in serum, bone, tooth, brain and liver; high concentration of aluminum can accumulate in serum, bone, tooth and brain (p0.05), and high concentration of fluoride can inhibit aluminum absorption. Brick tea increased fluoride content in bone, tooth and liver (p0.05) and aluminum content in tooth and liver (p0.05), and low fluoride Pu'er tea increased aluminum content in kidney and liver (p0.05). There were brain edema in brain tissue, proteinuria in kidney and small area of punctate necrosis of liver in fluoride-aluminum group and brick-tea group in the combination of fluoride-aluminum group and single-aluminum group, and the brain edema in brain tissue, proteinuria in the kidney of fluoride-aluminum combination group and single fluoride group. Conclusion 1. The animal model of brick tea-type fluoride-aluminum poisoning rats was successfully reproduced. 2. The target organs targeted by fluoride elements were bone and tooth and could cause corresponding damage. 2. The target organs targeted by aluminum elements are bone, tooth and brain, which can cause growth disorder and learning and memory dysfunction. 3. When fluoride and aluminum exist simultaneously, high concentration of aluminum can promote the absorption of fluoride, and high concentration of fluoride can inhibit the absorption of aluminum. Using low fluorine Pu'er tea instead of ordinary brick tea can prevent the poisoning of brick tea with fluorine and aluminum on the premise of not affecting the taste and color of brick tea.
【學(xué)位授予單位】：內(nèi)蒙古醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2009
【分類號(hào)】：R599;R-332

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 鄭新;;鋁對(duì)人體健康的影響及食品中鋁含量的測(cè)定[J];重慶科技學(xué)院學(xué)報(bào)(自然科學(xué)版);2007年01期

2 吳文華;吳文俊;;普洱茶對(duì)正常飲食小鼠血脂的影響[J];蠶桑茶葉通訊;2006年03期

3 李福成,周琳業(yè),方士杰,魏贊道,肖開琪,于燕妮,曹序茂,劉家騮,陳緒光;大白鼠鋁氟聯(lián)合中毒模型復(fù)制[J];地方病通報(bào);1989年01期

4 黃長(zhǎng)青,李明健;吉林飲水型與湖北燃煤污染型病區(qū)氟骨癥骨關(guān)節(jié)損害的放射學(xué)差異[J];中國(guó)地方病防治雜志;2001年03期

5 邢德利;徐淑艷;張文嵐;李廣生;;過量攝氟對(duì)大鼠腦組織氧化侵襲與細(xì)胞凋亡的影響[J];中國(guó)地方病防治雜志;2006年01期

6 劉慶斌;王五一;劉學(xué)慧;李海龍;李樹海;英殿雙;王革;王在君;王玉華;周明仁;潘晶巖;于愛民;李永華;王麗珍;;內(nèi)蒙古鄂溫克族牧民磚茶型氟鋁聯(lián)合中毒調(diào)查[J];中國(guó)地方病防治雜志;2006年05期

7 劉學(xué)慧;李海蓉;劉慶斌;王五一;王革;劉曉波;姜志立;李樹海;王麗珍;英殿雙;周明仁;田淑彩;潘晶巖;;內(nèi)蒙古陳巴爾虎旗飲茶型氟中毒病區(qū)牧民氟、鋁總攝入量調(diào)查[J];中國(guó)地方病防治雜志;2007年02期

8 展影,郭龍,孫興旺;鋁在阿爾茨海默病中的病因?qū)W作用[J];國(guó)外醫(yī)學(xué)(醫(yī)學(xué)地理分冊(cè));2004年04期

9 李奕,曹進(jìn);茶中鋁元素的安全性評(píng)估[J];國(guó)外醫(yī)學(xué)(醫(yī)學(xué)地理分冊(cè));2005年01期

10 陳群;王治倫;;氟骨癥與飲茶[J];國(guó)外醫(yī)學(xué)(醫(yī)學(xué)地理分冊(cè));2007年03期

，

本文編號(hào)：2444928