【摘要】：目的:觀察針刀干預(yù)椎動(dòng)脈型頸椎病(cervical spondylosis of vertebral artery type,CSA)模型兔肌肉組織光鏡下的形態(tài)學(xué)變化和血清一氧化氮(nitric oxide,NO)、血漿內(nèi)皮素(endothelin,ET)含量的影響,探討針刀干預(yù)椎動(dòng)脈型頸椎病的部分機(jī)制。方法:選用雌性新西蘭家兔25只,取5只作為空白對(duì)照組,剩余造模成功的15只隨機(jī)分為模型組、電針組、針刀組,各5只�？瞻捉M、模型組不干預(yù);電針組穴取"百會(huì)""風(fēng)池""頸1~3夾脊",針刺得氣后,連接華佗牌SDZ-Ⅱ型電子針灸儀,每次20 min,每天1次,10天為一療程,療程間休息1天,共2個(gè)療程;針刀組在C_3~C_5棘突旁開1.5~2 cm處予針刀治療,7天1次,共3次。觀察各組家兔頸部肌肉組織形態(tài)學(xué)變化,應(yīng)用硝酸還原酶法檢測(cè)各組血清NO的含量,采用放射免疫法檢測(cè)各組血漿ET的含量。結(jié)果:與電針組相比,光鏡下觀察針刀組干預(yù)后針切部位肌細(xì)胞輕度壞死,間質(zhì)間未見明顯的炎性細(xì)胞浸潤,針切斷裂部位趨向于正常骨骼肌及筋膜結(jié)構(gòu)結(jié)締組織修復(fù)。模型組血清NO含量低于空白對(duì)照組(P0.01),血漿ET含量高于空白對(duì)照組(P0.01);電針組與針刀組血清NO含量高于模型組(均P0.05),血漿ET含量低于模型組(均P0.05);針刀組家兔血漿ET含量明顯低于電針組(P0.05)。結(jié)論:針刀干預(yù)通過促進(jìn)炎性細(xì)胞消散,改善肌肉組織修復(fù),提升血清NO含量,降低血漿ET含量,使椎動(dòng)脈維持在一種正常的生理舒張狀態(tài),保證了腦組織的正常灌注,可能是針刀干預(yù)治療CSA的部分作用機(jī)制。
[Abstract]:Objective: to observe the morphological changes of muscle tissue and the level of serum nitric oxide (nitric oxide,NO) and plasma endothelin (endothelin,ET) in rabbits with cervical spondylosis of vertebral artery type (cervical spondylosis of vertebral artery type,CSA) treated by acupuncture knife. To explore the mechanism of acupuncture-knife intervention in cervical spondylosis of vertebral artery type. Methods: fifteen female New Zealand rabbits were randomly divided into three groups: model group (n = 15), electro-acupuncture group (n = 5), acupuncture group (n = 5) and blank control group (n = 5). Blank group, model group, non-intervention; In the electro-acupuncture group, "Baihui", "Fengchi" and "neck 1-3 clip ridge" were taken. After acupuncture, Huatuo brand SDZ- 鈪，

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