調(diào)胃承氣湯證和小承氣湯證兔動物模型的研制
發(fā)布時間：2019-03-04 13:04
【摘要】： 目的:研制出符合中醫(yī)陽明腑實證中的調(diào)胃承氣湯證和小承氣湯證的兔動物模型,建立穩(wěn)定的造模方法,并初步制定出該證的客觀證候指標(biāo),探討承氣輕下法的治療機理及意義。 方法:將105只白兔隨機分為正常對照組(1組)、調(diào)胃承氣湯證組(2組)、小承氣湯證組(3組),2、3組再各分為病理模型組A、模型治療組B、模型驗證組C,即(1、2A、2B、2C、3A、3B、3C),共七組。正常對照組給以正常飼料喂養(yǎng),自由飲水。調(diào)胃承氣湯證組和小承氣湯證組在實驗前48小時分別給予拌有不同劑量次碳酸鉍的飼料喂養(yǎng)2天,第2天禁水24小時。然后在自然狀態(tài)下,調(diào)胃承氣湯證組和小承氣湯證組分別注射不同劑量的大腸桿菌內(nèi)毒素。其中2A、3A組于攻毒前1小時、攻毒后2小時給予生理鹽水,2B、3B組在攻毒前1小時、攻毒后2小時分別灌胃以調(diào)胃承氣湯、小承氣湯,2C、3C組則在造模的同時分別給予調(diào)胃承氣湯和小承氣湯,直至實驗結(jié)束。正常對照組給予相應(yīng)比例的生理鹽水。在實驗中,觀測家兔的精神狀態(tài)、呼吸、耳血管、體溫、腹圍等一般情況。于攻毒后6小時心臟采血,檢測各項血液指標(biāo)。隨后處死動物,取肺、肝、腎、結(jié)腸等重要臟器作病理切片,光鏡觀察。 結(jié)果:正常對照組未見明顯的變化。模型組家兔(2A、3A)體溫明顯升高,以2A組升高最多;2A、3A組家兔腹圍顯著增大,以3A組增大更明顯。模型組的實驗指標(biāo)與正常對照組相比均有顯著差異(P＜0.01或P＜0.05),其中WBC、NO、MDA和MMS含量有不同程度的增高,SOD含量明顯下降;電解質(zhì)中鈉含量略有降低,鉀含量則顯著降低;全血粘度、血漿粘度升高,紅細(xì)胞變形指數(shù)降低;TXB_2、TM值和PAI-1含量明顯升高,同時t-PA含量明顯降低。治療組(2B、3B)、驗證組(2C、3C)與模型組比較均有顯著差異(P＜0.01或P＜0.05),與正常對照組比較,部分指標(biāo)無顯著差異,部分指標(biāo)仍有顯著差異(P＜0.01或P＜0.05)。病理形態(tài)大體觀察:模型組(2A、3A)大部分家兔的肺臟表面可見點狀或片狀出血點,大腸膨隆脹氣,腸管增粗,其余臟器未見明顯病變。光鏡觀察:模型組家兔肺臟均有改變,2A組家兔的肺可見局灶性肺實變,3A組稍輕,可見肺泡間隔增寬,炎細(xì)胞浸潤、充血。腸粘膜可見不同程度的炎細(xì)胞浸潤、充血、水腫。肝臟也可見不同程度的炎細(xì)胞浸潤,腎臟基本正常。治療組與驗證組家兔僅有輕微變化,或大致正常。 結(jié)論:采用次碳酸鉍、禁水和大腸桿菌內(nèi)毒素多因素聯(lián)合造模方法,通過控制給藥劑量,制作出不同的家兔病理模型,其主要癥狀、體征和病理變化符合《傷寒論》中調(diào)胃承氣湯證、小承氣湯證,故可以認(rèn)為用此種造模方法所復(fù)制的調(diào)胃承氣湯證、小承氣湯證動物模型是成功的。2、調(diào)胃承氣湯、小承氣湯可以有效降低模型組家兔的體溫,腹圍、減輕其癥狀和體征,改善各種實驗指標(biāo)和病理改變,保護(hù)臟器組織。因此可以反證此種造模方法切實可靠,所制作的調(diào)胃承氣湯證、小承氣湯證家兔動物模型是成功的。
[Abstract]:Objective: to develop a rabbit model of regulating stomach-bearing Qi decoction and Xiao Cheng-Qi decoction in accordance with the syndrome of Yang-Ming fu organs of traditional Chinese medicine, and to establish a stable model-making method, and preliminarily draw up the objective syndrome index of this syndrome. Objective: to explore the therapeutic mechanism and significance of qi-bearing and light-lowering therapy. Methods: 105 white rabbits were randomly divided into normal control group (group 1), Tiaowei Chengqi decoction syndrome group (group 2) and Xiaoshengqi decoction group (group 3). The three groups were divided into pathological model group A, model treatment group B, model verification group C, that is, (1, 2 A, 2), and 2, 3 groups were divided into pathological model group A, model treatment group B, model verification group C, respectively. 2B, 2C, 3A, 3B, 3C), there were seven groups. The normal control group was fed with normal feed and drinking water freely. The two groups were fed with different dosages of bismuth carbonate for2 days and 24 hours respectively at 48 hours before the experiment in the Tiaowei Chengqi decoction group and Xiaochengqi decoction syndrome group. The rats were fed with different doses of bismuth carbonate for2 days and 24 hours respectively. Then, under the natural condition, different doses of E. coli endotoxin were injected into the syndrome group of Tiaowei Chengqi decoction and the group of Xiaochengqi decoction respectively. 2A, 3A group was given normal saline 1 hour before and 2 hours after challenge, and 3B group was given intragastric administration of Tiaowei Chengqi decoction, Xiaochengqi decoction, 2C2C, respectively 1 hour before and 2 hours after virus attack, and 2B, 3B group was administered intragastrically to Tiaowei Chengqi decoction, Xiaochengqi decoction, 2C, respectively. 3C group was given Tiaowei Chengqi decoction and XiaoChengQi decoction respectively at the same time, until the end of the experiment. The normal control group was given the corresponding proportion of normal saline. In the experiment, we observed the general state of mind, breathing, auricular vessels, body temperature, abdominal circumference and so on. The blood samples were collected from the heart at 6 hours after the attack, and the blood indexes were measured. Then the animals were killed, lung, liver, kidney, colon and other important organs were taken for pathological sections and observed under light microscope. Results: there was no obvious change in normal control group. The body temperature of rabbits in model group (2A, 3A) was significantly increased, especially in 2A group, and the abdominal circumference of 3A group was significantly increased, especially in 3A group. The experimental indexes in the model group were significantly different from those in the normal control group (P < 0. 01 or P < 0. 05). The contents of WBC,NO,MDA and MMS in the model group were increased to some extent, while the content of SOD was significantly decreased in the model group. Sodium content in electrolyte decreased slightly, potassium content decreased significantly, whole blood viscosity, plasma viscosity increased, erythrocyte deformability index decreased, TXB_2, TM value and PAI-1 content increased, and t-PA content decreased significantly. There were significant differences between the treatment group (2B, 3B) and the verification group (2C, 3C) compared with the model group (P < 0.01 or P < 0.05), and there were no significant differences in some indexes between the treatment group (2B, 3B) and the model group (P < 0.05). There were still significant differences in some indexes (P < 0.01 or P < 0.05). Pathological observation: in the model group (2A, 3A), punctate or lamellar bleeding spots were observed on the surface of the lungs, bulging of the large intestine, thickening of the intestinal tube, and no obvious pathological changes were observed in the other organs of the rabbits in the model group (2A, 3A). Light microscopic observation: there were changes in the lungs of rabbits in the model group. Focal consolidation could be seen in the lungs of the rabbits in the 2A group and slightly lighter in the 3A group. Widening of the alveolar septum, infiltration of inflammatory cells and hyperemia were observed in the pulmonary septum of the rabbits in the model group. Intestinal mucosa can see different degrees of inflammatory cell infiltration, congestion, edema. Different degrees of inflammatory cell infiltration can also be seen in the liver, and the kidney is basically normal. There were only slight changes, or generally normal, in the treatment group and the verification group. Conclusion: bismuth hypocarbonate, water ban and endotoxin of Escherichia coli were used to model rabbits, and different pathological models of rabbits were made by controlling the dosage of the drug, the main symptoms of which were the main symptoms. The physical signs and pathological changes accord with the syndrome of Tiaowei Chengqi decoction and XiaoChengQi decoction in the Treatise of febrile Syndrome, so it can be considered that the animal model of Tiaowei Chengqi decoction and Xiaochengqi decoction copied by this modeling method is successful. 2, Tiaowei Chengqi decoction, Xiaochengqi decoction can effectively reduce the body temperature, abdominal circumference, relieve its symptoms and signs, improve various experimental indexes and pathological changes, and protect organs and tissues in the model group. Therefore, this kind of modeling method can be proved to be effective and reliable, and the animal models of Tiaowei Chengqi decoction and Xiaochengqi decoction are successful.
【學(xué)位授予單位】：福建中醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2008
【分類號】：R-332
本文編號：2434290
[Abstract]:Objective: to develop a rabbit model of regulating stomach-bearing Qi decoction and Xiao Cheng-Qi decoction in accordance with the syndrome of Yang-Ming fu organs of traditional Chinese medicine, and to establish a stable model-making method, and preliminarily draw up the objective syndrome index of this syndrome. Objective: to explore the therapeutic mechanism and significance of qi-bearing and light-lowering therapy. Methods: 105 white rabbits were randomly divided into normal control group (group 1), Tiaowei Chengqi decoction syndrome group (group 2) and Xiaoshengqi decoction group (group 3). The three groups were divided into pathological model group A, model treatment group B, model verification group C, that is, (1, 2 A, 2), and 2, 3 groups were divided into pathological model group A, model treatment group B, model verification group C, respectively. 2B, 2C, 3A, 3B, 3C), there were seven groups. The normal control group was fed with normal feed and drinking water freely. The two groups were fed with different dosages of bismuth carbonate for2 days and 24 hours respectively at 48 hours before the experiment in the Tiaowei Chengqi decoction group and Xiaochengqi decoction syndrome group. The rats were fed with different doses of bismuth carbonate for2 days and 24 hours respectively. Then, under the natural condition, different doses of E. coli endotoxin were injected into the syndrome group of Tiaowei Chengqi decoction and the group of Xiaochengqi decoction respectively. 2A, 3A group was given normal saline 1 hour before and 2 hours after challenge, and 3B group was given intragastric administration of Tiaowei Chengqi decoction, Xiaochengqi decoction, 2C2C, respectively 1 hour before and 2 hours after virus attack, and 2B, 3B group was administered intragastrically to Tiaowei Chengqi decoction, Xiaochengqi decoction, 2C, respectively. 3C group was given Tiaowei Chengqi decoction and XiaoChengQi decoction respectively at the same time, until the end of the experiment. The normal control group was given the corresponding proportion of normal saline. In the experiment, we observed the general state of mind, breathing, auricular vessels, body temperature, abdominal circumference and so on. The blood samples were collected from the heart at 6 hours after the attack, and the blood indexes were measured. Then the animals were killed, lung, liver, kidney, colon and other important organs were taken for pathological sections and observed under light microscope. Results: there was no obvious change in normal control group. The body temperature of rabbits in model group (2A, 3A) was significantly increased, especially in 2A group, and the abdominal circumference of 3A group was significantly increased, especially in 3A group. The experimental indexes in the model group were significantly different from those in the normal control group (P < 0. 01 or P < 0. 05). The contents of WBC,NO,MDA and MMS in the model group were increased to some extent, while the content of SOD was significantly decreased in the model group. Sodium content in electrolyte decreased slightly, potassium content decreased significantly, whole blood viscosity, plasma viscosity increased, erythrocyte deformability index decreased, TXB_2, TM value and PAI-1 content increased, and t-PA content decreased significantly. There were significant differences between the treatment group (2B, 3B) and the verification group (2C, 3C) compared with the model group (P < 0.01 or P < 0.05), and there were no significant differences in some indexes between the treatment group (2B, 3B) and the model group (P < 0.05). There were still significant differences in some indexes (P < 0.01 or P < 0.05). Pathological observation: in the model group (2A, 3A), punctate or lamellar bleeding spots were observed on the surface of the lungs, bulging of the large intestine, thickening of the intestinal tube, and no obvious pathological changes were observed in the other organs of the rabbits in the model group (2A, 3A). Light microscopic observation: there were changes in the lungs of rabbits in the model group. Focal consolidation could be seen in the lungs of the rabbits in the 2A group and slightly lighter in the 3A group. Widening of the alveolar septum, infiltration of inflammatory cells and hyperemia were observed in the pulmonary septum of the rabbits in the model group. Intestinal mucosa can see different degrees of inflammatory cell infiltration, congestion, edema. Different degrees of inflammatory cell infiltration can also be seen in the liver, and the kidney is basically normal. There were only slight changes, or generally normal, in the treatment group and the verification group. Conclusion: bismuth hypocarbonate, water ban and endotoxin of Escherichia coli were used to model rabbits, and different pathological models of rabbits were made by controlling the dosage of the drug, the main symptoms of which were the main symptoms. The physical signs and pathological changes accord with the syndrome of Tiaowei Chengqi decoction and XiaoChengQi decoction in the Treatise of febrile Syndrome, so it can be considered that the animal model of Tiaowei Chengqi decoction and Xiaochengqi decoction copied by this modeling method is successful. 2, Tiaowei Chengqi decoction, Xiaochengqi decoction can effectively reduce the body temperature, abdominal circumference, relieve its symptoms and signs, improve various experimental indexes and pathological changes, and protect organs and tissues in the model group. Therefore, this kind of modeling method can be proved to be effective and reliable, and the animal models of Tiaowei Chengqi decoction and Xiaochengqi decoction are successful.
【學(xué)位授予單位】：福建中醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2008
【分類號】：R-332
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