【摘要】： 抵抗素是一種與胰島素抵抗和2型糖尿病密切相關的分泌型蛋白,在白色脂肪組織中起重要調節(jié)作用,作為新發(fā)現的基因之一,其功能尚未完全清楚。抵抗素對嚙齒類動物和人類的作用存在一定爭議,抵抗素對糖、脂代謝及脂肪組織內的炎癥反應一直是人們關注的焦點問題。目前未見文獻報道抵抗素過量表達對人脂肪干細胞有何影響及其機制。本研究分離、鑒定成人脂肪干細胞,檢測其向成熟脂肪細胞分化過程中不同階段抵抗素表達,首次應用基因工程重組技術構建攜帶人類抵抗素基因的慢病毒顆粒Lv-hR并用其感染脂肪干細胞,觀察抵抗素轉染對脂肪干細胞形態(tài)、生長和分化能力的影響。研究抵抗素對脂肪干細胞的作用及機制,闡述抵抗素與肥胖和胰島素抵抗的關系。研究結果表明：成人脂肪干細胞向成熟脂肪細胞分化過程中抵抗素表達不斷下降；抵抗素過表達可使脂肪干細胞分化受抑制,但細胞數量未見明顯改變。
[Abstract]:Resistin, a secretory protein closely related to insulin resistance and type 2 diabetes, plays an important role in the regulation of white adipose tissue. As one of the newly discovered genes, its function has not been fully understood. The role of resistin in rodents and humans has been controversial. Resistin has been the focus of attention on glucose, lipid metabolism and inflammation in adipose tissue. There is no literature about the effect of resistin overexpression on human adipose stem cells and its mechanism. In this study, adult adipose stem cells were isolated and identified, and the expression of resistin in different stages of differentiation into mature adipocytes was detected. The lentivirus particle Lv-hR carrying human resistin gene was constructed for the first time and infected with adipose stem cells. The effects of resistin transfection on the morphology, growth and differentiation of adipose stem cells were observed. To study the effect and mechanism of resistin on adipose stem cells and to elucidate the relationship between resistin and obesity and insulin resistance. The results showed that the expression of resistin decreased continuously during the differentiation of adult adipose stem cells into mature adipocytes, and the over-expression of resistin could inhibit the differentiation of adipose stem cells, but the number of adipose stem cells did not change significantly.
【學位授予單位】：吉林大學
【學位級別】：碩士
【學位授予年份】：2010
【分類號】：R329

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本文編號：2428847