【摘要】： 目的探討骨髓源神經(jīng)干細(xì)胞(NSCs)對神經(jīng)膠質(zhì)瘤的定向遷移能力及分布規(guī)律。方法從雄性Wistar大鼠股骨和脛骨分離純化骨髓基質(zhì)干細(xì)胞(BMSCs),加堿性成纖維細(xì)胞生長因子(bFGF)和表皮生長因子(EGF)誘導(dǎo)分化為NSCs,并進(jìn)行免疫組化鑒定。立體定向接種C6膠質(zhì)瘤細(xì)胞于Wistar大鼠腦內(nèi)基底節(jié)區(qū)建立鼠腦膠質(zhì)瘤模型,7d后將用5-溴脫氧尿嘧啶(BrdU)標(biāo)記的RSCs接種入鼠腦對側(cè)半球,對側(cè)腦室和鼠尾靜脈,NSCs移植14d后心臟灌注取腦制成石蠟切片,以蘇木素伊紅(HE)染色確定膠質(zhì)瘤性質(zhì),SABC法免疫熒光染色顯示NSCs對膠質(zhì)瘤組織的定向遷移能力、分布規(guī)律及其遷移過程中存活和分化情況等。結(jié)果對側(cè)半球組和對側(cè)腦室組均可見移植的NSCs多沿著注射途徑直線分布,并可見移植細(xì)胞沿著胼胝體,腦室,血管向?qū)?cè)膠質(zhì)瘤組織遷移。在膠質(zhì)瘤與正常組織交界處可見激發(fā)出綠色熒光的陽性NSCs,鼠尾靜脈組亦可見少量陽性細(xì)胞分布在膠質(zhì)瘤組織周圍。結(jié)論骨髓源NSCs對膠質(zhì)瘤組織具有明顯的趨化作用,能通過遷移準(zhǔn)確定位于膠質(zhì)瘤組織內(nèi)。為以骨髓源NSCs為細(xì)胞載體的靶向治療提供了實(shí)驗(yàn)依據(jù)。
[Abstract]:Objective to investigate the directional migration ability and distribution of bone marrow derived neural stem cells (NSCs) on gliomas. Methods Bone marrow stromal cells (BMSCs), basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) were isolated and purified from femur and tibia of male Wistar rats. The glioma model was established by stereotactic inoculation of C6 glioma cells in the basal ganglia of Wistar rats. After 7 days, 5-bromodeoxyuridine (5-bromodeoxyuridine) labeled RSCs was inoculated into the contralateral hemisphere, contralateral ventricle and caudal vein of the rat. After 14 days of NSCs transplantation, the brain was perfused to make paraffin sections, and the nature of gliomas was determined by hematoxylin eosin (HE) staining. SABC immunofluorescence staining showed the directional migration ability of NSCs to glioma tissues. Distribution law and survival and differentiation during migration. Results in the contralateral hemisphere group and contralateral ventricular group, most of the transplanted NSCs were distributed linearly along the injection route, and the transplanted cells migrated to the contralateral glioma tissue along the corpus callosum, ventricle and blood vessels. At the junction of glioma and normal tissues, a small number of positive cells could also be seen around the glioma tissues in the group of the tail vein of NSCs, mice, which excited green fluorescence. Conclusion NSCs derived from bone marrow has obvious chemotactic effect on glioma tissue and can be accurately located in glioma tissue by migration. It provides experimental basis for targeted therapy with bone marrow derived NSCs as cell carrier.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2008
【分類號】：R329

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