【摘要】：近年來,金納米粒子因其具有很多宏觀粒子所不具備的小尺寸效應、表面效應、光學效應以及特殊的生物親和效應,已經廣泛應用于臨床醫(yī)學、材料學等領域。而且在醫(yī)學檢驗領域,金納米粒子標記技術成為目前第四大標記技術。金納米粒子所具備的特殊的物理與化學性質,在許多研究領域中表現(xiàn)出了潛在的應用價值。目前,金納米粒子的分析應用主要是通過化學修飾后作為光譜探針與其它物質結合反應,但是用不經過任何化學改性的金納米粒子作探針,結合光度法或RRS法測定藥物的分析方法報道還很少見。研究的主要內容有： 綜述了金納米粒子的制備和表征方法、光譜性質及其在分析化學中的應用和抗癌藥物6-巰基嘌呤的性質、作用及分析現(xiàn)狀等。 利用金納米粒子作探針,建立了一種分光光度法檢測微量抗癌藥物6-巰基嘌呤的新方法。6-巰基嘌呤自組裝到金納米粒子上以后,溶液的顏色由亮紅色變?yōu)樯钏{色,吸收光譜發(fā)生明顯的變化。反應前,金納米粒子在520 nm處出現(xiàn)了其典型的等離子共振吸收帶,而6-巰基嘌呤在可見區(qū)無吸收峰；反應后,520 nm吸收峰處的吸光度降低,而在680 nm處出現(xiàn)一較強吸收峰,該吸收峰為結合產物的待征吸收峰。并且,在該波長下吸光度與6-巰基嘌呤的濃度呈良好線性關系,檢出限為17μg/L。對濃度為0.25mg/L的6-巰基嘌呤進行了11次平行測定,RSD為2.9%。將該法用于檢測人尿樣中的6-巰基嘌呤含量,方法簡便、靈敏度高、抗干擾性強,檢測結果令人滿意。 以金納米粒子為探針,建立了一種共振瑞利散射光譜法檢測6-巰基嘌呤的新方法。在pH為5.50的B-R緩沖溶液中,金納米粒子與6-巰基嘌呤自組裝成體積更大的結合產物,從而使體系的共振瑞利散射光譜強度急劇增強。在實驗中,研究了其共振瑞利散射光譜特征,并且考察了影響反應的一些因素和共存物質的影響。結果表明：在最佳條件下,6-巰基嘌呤濃度在0.017～0.32 mg/L范圍內與共振瑞利散射光譜強度成線性關系,方法的靈敏度較高,其檢出限(3σ)為8μg/L,對濃度為0.12mg/L的6-巰基嘌呤進行了11次平行測定,RSD為5.1%。考察共存物質對6-MP測定的影響,發(fā)現(xiàn)該方法具有較好的選擇性。根據所確定的最佳條件,建立了一種共振瑞利散射光譜法檢測6-巰基嘌呤的新方法,并將該方法用于人尿樣中6-巰基嘌呤含量的測定,獲得較好的結果。
[Abstract]:In recent years, gold nanoparticles have been widely used in clinical medicine, materials science and other fields because of their small size effect, surface effect, optical effect and special biological affinity effect that many macroscopical particles do not have. And in the field of medical testing, gold nanoparticles labeling technology has become the fourth largest marking technology. Gold nanoparticles have special physical and chemical properties, which have shown potential application value in many research fields. At present, the analysis of gold nanoparticles is mainly through chemical modification as a spectral probe to react with other substances, but not through any chemical modification of gold nanoparticles as a probe, Analytical methods combined with photometry or RRS are rarely reported. The main contents of this paper are as follows: the preparation and characterization of gold nanoparticles, the spectral properties and their applications in analytical chemistry, the properties, functions and analytical status of the anticancer drug 6-mercaptopurine are reviewed. A new spectrophotometric method for the determination of trace antitumor drug 6-mercaptopurine was established using gold nanoparticles as a probe. After self-assembly of 6-mercaptopurine onto gold nanoparticles, the color of the solution changed from bright red to dark blue. The absorption spectrum changed obviously. Before the reaction, gold nanoparticles appeared their typical plasmon resonance absorption band at 520 nm, while 6-mercaptopurine had no absorption peak in visible region. After the reaction, the absorbance at the absorption peak of 520 nm decreased, but a strong absorption peak appeared at 680 nm, which was the absorption peak of the bound product. Moreover, the absorbance has a good linear relationship with the concentration of 6-mercaptopurine at this wavelength, and the detection limit is 17 渭 g 路L ~ (-1) 路L ~ (-1). The concentration of 6-mercaptopurine with 0.25mg/L was determined for 11 times, and RSD was 2.9. The method has been applied to the determination of 6-mercaptopurine in human urine. The method is simple, sensitive, anti-interference and satisfactory. A new method for the determination of 6-mercaptopurine by resonance Rayleigh scattering spectroscopy was developed using gold nanoparticles as probe. In B-R buffer solution with pH of 5.50, the gold nanoparticles self-assembled with 6-mercaptopurine to form a larger volume, thus the resonance Rayleigh scattering spectra of the system were greatly enhanced. In the experiment, the resonance Rayleigh scattering spectra were studied, and some factors affecting the reaction and the influence of coexisting substances were investigated. The results show that under the optimum conditions, the concentration of 6-mercaptopurine is linearly related to the resonance Rayleigh scattering intensity in the range of 0.017 ~ 0.32 mg/L. The sensitivity of the method is high, and the detection limit (3 蟽) is 8 渭 g / L. The concentration of 6-mercaptopurine with 0.12mg/L was determined for 11 times, and RSD was 5.1. The effect of coexisting substances on the determination of 6-MP was investigated, and the method was found to have good selectivity. A new method for the determination of 6-mercaptopurine by resonance Rayleigh scattering spectroscopy was established, and the method was applied to the determination of 6-mercaptopurine in human urine with good results.
【學位授予單位】：東北大學
【學位級別】：碩士
【學位授予年份】：2008
【分類號】：R341

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