【摘要】：目的探討大豆異黃酮(SI)代謝物雌馬酚對(duì)血管內(nèi)皮細(xì)胞凋亡和增殖的影響。方法體外培養(yǎng)血管內(nèi)皮細(xì)胞(ECV-304),采用不同濃度雌馬酚(0、6.25、12.50、25.00、50.00和100.00μmol/L)處理24 h,收集細(xì)胞,采用流式細(xì)胞術(shù)分析細(xì)胞內(nèi)活性氧(ROS)、細(xì)胞凋亡和細(xì)胞周期;采用免疫組織化學(xué)技術(shù)分析凋亡相關(guān)蛋白Bax、Bcl-2和caspase-3表達(dá);采用噻唑藍(lán)(MTT)法分析雌馬酚對(duì)ECV-304細(xì)胞增殖影響。結(jié)果與對(duì)照組比較,6.25μmol/L雌馬酚組ECV-304細(xì)胞內(nèi)ROS含量下降,100.00μmol/L雌馬酚組ECV-304細(xì)胞內(nèi)ROS含量升高;與對(duì)照組比較,25.00、50.00、100.00μmol/L雌馬酚組ECV-304細(xì)胞早、晚期凋亡率明顯升高,并可導(dǎo)致細(xì)胞G1期阻滯(P0.05);MTT實(shí)驗(yàn)結(jié)果顯示,0、6.25、12.50、25.00、50.00、100.00μmol/L雌馬酚組ECV-304細(xì)胞增殖抑制率分別為-(8.23±2.21)%、-(3.29±1.07)%、(6.75±1.73)%、(25.46±4.82)%和(36.93±4.66)%,低劑量雌馬酚對(duì)細(xì)胞生長(zhǎng)具有促進(jìn)作用,高劑量雌馬酚對(duì)細(xì)胞生長(zhǎng)具有抑制作用;與對(duì)照組比較,25.00μmol/L雌馬酚組作用24 h后,細(xì)胞內(nèi)Bax和caspase-3表達(dá)增加,而Bcl-2表達(dá)減少。結(jié)論低濃度雌馬酚可降低細(xì)胞內(nèi)ROS含量,促進(jìn)ECV-304細(xì)胞增殖;高濃度雌馬酚可導(dǎo)致細(xì)胞凋亡、細(xì)胞周期阻滯,抑制細(xì)胞增殖。
[Abstract]:Objective to investigate the effect of estradiol, a metabolite of soybean isoflavone (SI), on apoptosis and proliferation of vascular endothelial cells. Methods Vascular endothelial cells (ECV-304) were cultured in vitro and treated with different concentrations of estradiol (0.6.25A12.5012.50,25.0050.00 and 100.00 渭 mol/L) for 24 hours. The cells were collected and analyzed by flow cytometry for intracellular reactive oxygen species (ROS),). Apoptosis and cell cycle; The expression of apoptosis-related protein Bax,Bcl-2 and caspase-3 was analyzed by immunohistochemistry and the effect of estradiol on the proliferation of ECV-304 cells was analyzed by thiazolyl (MTT) assay. Results compared with the control group, the content of ROS in ECV-304 cells in 6.25 渭 mol/L female equisol group decreased, and the ROS content in ECV-304 cells increased in 100.00 渭 mol/L estralophen group. Compared with the control group, the ECV-304 cells in the 25.00 渭 mol/L 50.00100.00 渭 mol/L group were early, the late apoptosis rate was significantly increased, and the G1 phase arrest was induced (P0.05). The results of MTT test showed that the inhibition rate of ECV-304 cell proliferation was-(8.23 鹵2.21)%, (3.29 鹵1.07)%, (6.75 鹵1.73)% and (6.75 鹵1.73)%, respectively. (25.46 鹵4.82)% and (36.93 鹵4.66)% respectively. Low dose of estradiol promoted cell growth, and high dose of female equisol inhibited cell growth. Compared with the control group, the expression of Bax and caspase-3 increased and the expression of Bcl-2 decreased in 25.00 渭 mol/L female equallol group for 24 h. Conclusion low concentration of estradiol can reduce the content of ROS and promote the proliferation of ECV-304 cells, and high concentration of estradiol can induce cell apoptosis, arrest cell cycle and inhibit cell proliferation.
【作者單位】： 吉林醫(yī)藥學(xué)院心理學(xué)教研室;吉林醫(yī)藥學(xué)院檢驗(yàn)學(xué)院;吉林醫(yī)藥學(xué)院藥學(xué)院;
【基金】：吉林省教育廳“十二五”科學(xué)技術(shù)研究項(xiàng)目(2012335) 國(guó)家自然科學(xué)基金(82102953) 吉林省科技廳中青年科技創(chuàng)新領(lǐng)軍人才及團(tuán)隊(duì)項(xiàng)目(20130521018JH) 吉林省科技廳科技攻關(guān)重大項(xiàng)目(20140203012YY)
【分類號(hào)】：R3411

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