發(fā)布時(shí)間：2018-12-11 16:45

【摘要】： 轉(zhuǎn)基因技術(shù)對(duì)推動(dòng)現(xiàn)代生物學(xué)和醫(yī)學(xué)發(fā)展產(chǎn)生了巨大的作用。轉(zhuǎn)基因小鼠技術(shù)已經(jīng)成為了一種常規(guī)的實(shí)驗(yàn)室技術(shù),目前已經(jīng)建立了數(shù)千種不同的轉(zhuǎn)基因小鼠模型,為生物醫(yī)學(xué)研究提供了有效的在體模型工具。但轉(zhuǎn)基因大鼠的研究在國內(nèi)卻是進(jìn)展緩慢,轉(zhuǎn)基因效率很低,為了提高轉(zhuǎn)基因大鼠的效率,本研究對(duì)不同組合PMSG和HCG對(duì)不同周齡大鼠超排后胚胎數(shù)量、胚胎質(zhì)量、原核注射后存活率和胚胎移植成功率等進(jìn)行了系統(tǒng)分析,通過三種不同基因轉(zhuǎn)基因大鼠模型構(gòu)建成功建立了原核注射轉(zhuǎn)基因大鼠技術(shù)。在此基礎(chǔ)上我們又將pBC-SV40Tag注射到SD大鼠胚胎原核中,經(jīng)PCR和Southern檢測(cè)成功獲得了SV40Tag陽性轉(zhuǎn)基因大鼠。RT-PCR及westhern bloting結(jié)果表明:pBC-SV40Tag轉(zhuǎn)基因大鼠不僅如預(yù)期的在胰腺組織表達(dá)SV40Tag,同時(shí)也在腦中高表達(dá)SV40Tag,并成功獲得了腦腫瘤轉(zhuǎn)基因大鼠模型。
[Abstract]:Transgenic technology plays an important role in promoting the development of modern biology and medicine. Transgenic mouse technology has become a conventional laboratory technology, and thousands of different transgenic mouse models have been established, which provides an effective in vivo model tool for biomedical research. In order to improve the efficiency of transgenic rats, the number and quality of superovulation embryos in different combinations of PMSG and HCG were studied. The survival rate after prokaryotic injection and the success rate of embryo transfer were systematically analyzed. The technique of prokaryotic injection transgenic rats was successfully established through the construction of three transgenic rat models with different genes. On this basis, we injected pBC-SV40Tag into the prokaryotic cells of SD rat embryos. SV40Tag positive transgenic rats were successfully obtained by PCR and Southern. The results of RT-PCR and westhern bloting showed that pBC-SV40Tag transgenic rats expressed SV40Tag, not only in pancreas as expected, but also in brain. The transgenic rat model of brain tumor was successfully obtained.
【學(xué)位授予單位】：揚(yáng)州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2009
【分類號(hào)】：R739.4;R-332

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