【摘要】： 轉基因技術對推動現(xiàn)代生物學和醫(yī)學發(fā)展產生了巨大的作用。轉基因小鼠技術已經成為了一種常規(guī)的實驗室技術,目前已經建立了數(shù)千種不同的轉基因小鼠模型,為生物醫(yī)學研究提供了有效的在體模型工具。但轉基因大鼠的研究在國內卻是進展緩慢,轉基因效率很低,為了提高轉基因大鼠的效率,本研究對不同組合PMSG和HCG對不同周齡大鼠超排后胚胎數(shù)量、胚胎質量、原核注射后存活率和胚胎移植成功率等進行了系統(tǒng)分析,通過三種不同基因轉基因大鼠模型構建成功建立了原核注射轉基因大鼠技術。在此基礎上我們又將pBC-SV40Tag注射到SD大鼠胚胎原核中,經PCR和Southern檢測成功獲得了SV40Tag陽性轉基因大鼠。RT-PCR及westhern bloting結果表明:pBC-SV40Tag轉基因大鼠不僅如預期的在胰腺組織表達SV40Tag,同時也在腦中高表達SV40Tag,并成功獲得了腦腫瘤轉基因大鼠模型。
[Abstract]:Transgenic technology plays an important role in promoting the development of modern biology and medicine. Transgenic mouse technology has become a conventional laboratory technology, and thousands of different transgenic mouse models have been established, which provides an effective in vivo model tool for biomedical research. In order to improve the efficiency of transgenic rats, the number and quality of superovulation embryos in different combinations of PMSG and HCG were studied. The survival rate after prokaryotic injection and the success rate of embryo transfer were systematically analyzed. The technique of prokaryotic injection transgenic rats was successfully established through the construction of three transgenic rat models with different genes. On this basis, we injected pBC-SV40Tag into the prokaryotic cells of SD rat embryos. SV40Tag positive transgenic rats were successfully obtained by PCR and Southern. The results of RT-PCR and westhern bloting showed that pBC-SV40Tag transgenic rats expressed SV40Tag, not only in pancreas as expected, but also in brain. The transgenic rat model of brain tumor was successfully obtained.
【學位授予單位】：揚州大學
【學位級別】：碩士
【學位授予年份】：2009
【分類號】：R739.4;R-332

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