在皂甙和丙烯酰胺誘導(dǎo)下海兔腦神經(jīng)節(jié)及神經(jīng)連索的差異蛋白質(zhì)組研究
發(fā)布時間：2018-12-11 16:35
【摘要】： 海兔(Aplysia)歸屬于腹足綱軟體動物,它的中樞神經(jīng)系統(tǒng)(central nervoussystem,CNS)是開展分子神經(jīng)生物學(xué)研究的經(jīng)典模式之一。研究海兔CNS功能蛋白質(zhì)和多肽結(jié)構(gòu)與功能有助于科學(xué)地揭示人及高等動物神經(jīng)系統(tǒng)的活動規(guī)律和調(diào)控機制,例如:記憶形成、神經(jīng)性生理調(diào)控和神經(jīng)系統(tǒng)疾病起因等。三七(Panax notoginseng,PN)是中國傳統(tǒng)中草藥之一,其主要成分為皂甙,具有促智、延緩衰老等保護神經(jīng)系統(tǒng)功能。 采用薄層層析(TLC)、高效液相色譜(HPLC)和基質(zhì)輔助激光解吸附飛行時間質(zhì)譜(MALDI-TOF-MS)分析三七提取液(Panax notoginseng extract,PNE)組成,指出三七的主要成分為人參皂甙Rb1、Rg1、Re和三七皂甙R1,占總皂甙80%以上。 選用藍斑背綱海兔(Notarcus Ieachii cirrosus Stimpson,NLCS)的CNS為實驗材料,采用蛋白質(zhì)組技術(shù),初步鑒定了NLCS受PNE誘導(dǎo)后,其神經(jīng)連索表達差異蛋白有肌動蛋白、3-羥酯酰輔酶A脫氫酶、ABC轉(zhuǎn)運子、甲基轉(zhuǎn)移酶等。經(jīng)R1誘導(dǎo)后,其神經(jīng)連索表達的差異蛋白是凋亡抑制蛋白、26S蛋白酶體、酰基輔酶A脫氫酶和甲基轉(zhuǎn)移酶等。為了獲得更詳細(xì)的信息,對這些差異蛋白進行了亞細(xì)胞定位,通過比對分析后,作者認(rèn)為這些差異蛋白可能與學(xué)習(xí)記憶,神經(jīng)系統(tǒng)疾病有關(guān)。闡明PNE和R1的作用機制起著重要的作用,其結(jié)果可為今后開展神經(jīng)疾病預(yù)防和治療提供科學(xué)依據(jù)和新穎途徑。 丙烯酰胺(Acrylamide,ACR)是一種常見的神經(jīng)毒劑,它直接損傷神經(jīng)系統(tǒng)。三七皂甙組成能起到減緩神經(jīng)損傷的作用。選用雜斑海兔(Aplysia juliana,AJ)的CNS為實驗材料,篩選出AJ經(jīng)ACR毒害后,PNE對AJ大腦神經(jīng)節(jié)產(chǎn)生恢復(fù)(拮抗)過程中的差異蛋白質(zhì)。指出AJ的大腦神經(jīng)節(jié)經(jīng)ACR毒害后,產(chǎn)生了24個差異蛋白,但經(jīng)PNE恢復(fù)處理后,產(chǎn)生了拮抗效應(yīng),消除了多數(shù)差異蛋白質(zhì)的表達,使其表達量接近對照組,PNE+ACR和ACR+PNE的效果并不完全一樣。經(jīng)過數(shù)據(jù)庫檢索,發(fā)現(xiàn)有3個蛋白質(zhì)已經(jīng)報道與神經(jīng)系統(tǒng)損傷有關(guān),分別是熱休克蛋白20、磷酸丙糖異構(gòu)酶和短鏈脫氫酶。此研究有助于闡明ACR在腦神經(jīng)節(jié)代謝過程中所產(chǎn)生的毒性機理,以及探討PNE在保護ACR毒性效應(yīng)的方式,對實踐應(yīng)用具有指導(dǎo)意義。
[Abstract]:(Aplysia) belongs to Gastropoda mollusc and its central nervous system (central nervoussystem,CNS) is one of the classical models of molecular neurobiology. The study of the structure and function of CNS functional proteins and polypeptides in sea rabbits is helpful to reveal the activity and regulation mechanism of nervous system in human and higher animals, such as memory formation, neurophysiological regulation and the causes of nervous system diseases. Panax notoginseng (Panax notoginseng,PN) is one of the traditional Chinese herbal medicines, its main component is saponin, which has the function of promoting intelligence, delaying senescence and protecting nervous system. (TLC), high performance liquid chromatography (HPLC) and matrix assisted laser desorption time of flight mass spectrometry (MALDI-TOF-MS) were used to analyze the composition of Panax notoginseng extract (Panax notoginseng extract,PNE. It was pointed out that the main component of Panax notoginseng was ginsenoside Rb1,Rg1,. Re and Panax notoginsenoside R 1 accounted for more than 80% of the total saponins. The CNS of (Notarcus Ieachii cirrosus Stimpson,NLCS was selected as the experimental material, and the differential expression of actin and 3-hydroxyester coenzyme A dehydrogenase in the neuronal connectors of NLCS induced by PNE were preliminarily identified by proteome technique. ABC transporter, methyltransferase, etc. After induction by R1, the differentially expressed proteins were apoptosis inhibitor protein, 26s proteasome, acyl coenzyme A dehydrogenase and methyltransferase. In order to obtain more detailed information, subcellular localization of these differential proteins was carried out. After comparative analysis, the authors concluded that these differential proteins might be related to learning, memory and nervous system diseases. The mechanism of PNE and R1 plays an important role, and the results may provide scientific basis and novel approaches for the prevention and treatment of neurological diseases in the future. Acrylamide (Acrylamide,ACR) is a common nerve agent that directly damages the nervous system. The composition of Panax notoginseng saponins can attenuate the nerve injury. The CNS of Aplysia juliana,AJ rabbits was used as the experimental material to screen the differential proteins in the recovery (antagonism) of AJ brain ganglion induced by PNE after AJ was poisoned by ACR. It was pointed out that after being poisoned by ACR, 24 differentially expressed proteins were produced in AJ's cerebral ganglion, but after PNE was restored, antagonistic effect was produced, and the expression of most differentially expressed proteins was eliminated, and the expression of most differentially expressed proteins was close to that of the control group. The effects of PNE ACR and ACR PNE are not exactly the same. By searching the database, we found that three proteins have been reported to be related to nervous system injury, namely heat shock protein 20, propanoisomerase phosphate and short chain dehydrogenase. This study is helpful to clarify the toxic mechanism of ACR in the metabolism of cerebral ganglion, and to explore the way of protecting ACR toxicity by PNE, which is of guiding significance for practical application.
【學(xué)位授予單位】：廈門大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2008
【分類號】：R285.5;R363
本文編號：2372873
[Abstract]:(Aplysia) belongs to Gastropoda mollusc and its central nervous system (central nervoussystem,CNS) is one of the classical models of molecular neurobiology. The study of the structure and function of CNS functional proteins and polypeptides in sea rabbits is helpful to reveal the activity and regulation mechanism of nervous system in human and higher animals, such as memory formation, neurophysiological regulation and the causes of nervous system diseases. Panax notoginseng (Panax notoginseng,PN) is one of the traditional Chinese herbal medicines, its main component is saponin, which has the function of promoting intelligence, delaying senescence and protecting nervous system. (TLC), high performance liquid chromatography (HPLC) and matrix assisted laser desorption time of flight mass spectrometry (MALDI-TOF-MS) were used to analyze the composition of Panax notoginseng extract (Panax notoginseng extract,PNE. It was pointed out that the main component of Panax notoginseng was ginsenoside Rb1,Rg1,. Re and Panax notoginsenoside R 1 accounted for more than 80% of the total saponins. The CNS of (Notarcus Ieachii cirrosus Stimpson,NLCS was selected as the experimental material, and the differential expression of actin and 3-hydroxyester coenzyme A dehydrogenase in the neuronal connectors of NLCS induced by PNE were preliminarily identified by proteome technique. ABC transporter, methyltransferase, etc. After induction by R1, the differentially expressed proteins were apoptosis inhibitor protein, 26s proteasome, acyl coenzyme A dehydrogenase and methyltransferase. In order to obtain more detailed information, subcellular localization of these differential proteins was carried out. After comparative analysis, the authors concluded that these differential proteins might be related to learning, memory and nervous system diseases. The mechanism of PNE and R1 plays an important role, and the results may provide scientific basis and novel approaches for the prevention and treatment of neurological diseases in the future. Acrylamide (Acrylamide,ACR) is a common nerve agent that directly damages the nervous system. The composition of Panax notoginseng saponins can attenuate the nerve injury. The CNS of Aplysia juliana,AJ rabbits was used as the experimental material to screen the differential proteins in the recovery (antagonism) of AJ brain ganglion induced by PNE after AJ was poisoned by ACR. It was pointed out that after being poisoned by ACR, 24 differentially expressed proteins were produced in AJ's cerebral ganglion, but after PNE was restored, antagonistic effect was produced, and the expression of most differentially expressed proteins was eliminated, and the expression of most differentially expressed proteins was close to that of the control group. The effects of PNE ACR and ACR PNE are not exactly the same. By searching the database, we found that three proteins have been reported to be related to nervous system injury, namely heat shock protein 20, propanoisomerase phosphate and short chain dehydrogenase. This study is helpful to clarify the toxic mechanism of ACR in the metabolism of cerebral ganglion, and to explore the way of protecting ACR toxicity by PNE, which is of guiding significance for practical application.
【學(xué)位授予單位】：廈門大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2008
【分類號】：R285.5;R363
【引證文獻】
相關(guān)博士學(xué)位論文 前1條
1 應(yīng)劍;藥物活性評價、代謝分析和晶型技術(shù)在藥物發(fā)現(xiàn)中的應(yīng)用研究[D];北京協(xié)和醫(yī)學(xué)院;2011年
相關(guān)碩士學(xué)位論文 前1條
1 薛曉昀;大豆根瘤菌與促生菌復(fù)合系篩選及機理研究[D];中國農(nóng)業(yè)科學(xué)院;2011年
，本文編號：2372873
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