【摘要】： 重組激活基因RAGs產(chǎn)物的正確表達,對于淋巴細胞成熟后的結(jié)構(gòu)和功能有著重要的作用,是Ig和TCR多樣性的主要原因,在免疫應(yīng)答中發(fā)揮重要作用。RAGs基因的突變和異常表達可引起嚴重的免疫缺陷疾病。本文采用RT-PCR方法從人類RAGs表達細胞中擴增RAG-2編碼蛋白質(zhì)cDNA,插入到真核表達載體,構(gòu)建RAG-2的真核表達載體,并在體外轉(zhuǎn)染人類RAG-2非表達細胞系。表達載體DNA測序結(jié)果顯示與GeneBank已知序列完全一致,RAG-2基因已被正確插入到表達載體中;在轉(zhuǎn)染細胞中用RT-PCR法檢測到RAG-2 mRNA的表達。RAG-2表達載體的成功構(gòu)建,為進一步在人類淋巴細胞中表達出有功能的RAGs分子的研究奠定了基礎(chǔ)、為探討RAGs表達異常所致免疫缺陷病的基因治療方法提供理論依據(jù)。
[Abstract]:The correct expression of recombinant activated gene RAGs products plays an important role in the structure and function of lymphocytes after maturation, and is the main reason for the diversity of Ig and TCR. The mutation and abnormal expression of RAGs gene can lead to serious immune deficiency disease. RAG-2 encoding protein cDNA, was amplified from human RAGs expression cells by RT-PCR method and inserted into eukaryotic expression vector. The eukaryotic expression vector of RAG-2 was constructed and transfected into human RAG-2 non-expression cell line in vitro. The results of DNA sequencing of the expression vector showed that the RAG-2 gene had been inserted into the expression vector correctly. The expression of RAG-2 mRNA was detected by RT-PCR method in transfected cells. The successful construction of RAG-2 expression vector laid a foundation for the further study of the expression of functional RAGs molecules in human lymphocytes. To provide theoretical basis for gene therapy of immunodeficiency due to abnormal expression of RAGs.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2008
【分類號】：R392

【共引文獻】

相關(guān)期刊論文 前2條

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相關(guān)碩士學(xué)位論文 前2條

1 劉旭;人類重組激活基因-1（RAG-1）的表達研究[D];吉林大學(xué);2008年

2 張雪利;紅笛鯛重組激活基因克隆及表達分析[D];廣東海洋大學(xué);2012年

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本文編號：2364692