發(fā)布時間：2018-11-28 18:15

【摘要】：目的：觀察補腎活血法對大鼠慢性高眼壓(elevated intraocular pressure, EIOP)模型外側膝狀體(lateral geniculate nucleus,LGN)損傷的干預作用,初步探討補腎活血法對其的作用機理。 方法：采用烙閉上鞏膜靜脈法,烙閉大鼠3支上鞏膜靜脈,建立大鼠慢性EIOP模型,隨機分為3組：空白組,模型組,給藥組。連續(xù)灌胃8周,并于8周末處死大鼠,觀察補腎活血法對EIOP大鼠眼壓(intraocular pressure, IOP)、LGN病理形態(tài)學變化以及對腦源性神經(jīng)營養(yǎng)因子(brain-derived neurotrophin factor, BDNF)表達的影響。 結果：本實驗采用的烙閉上鞏膜靜脈的造模方法使大鼠IOP明顯升高(P0.01),在造模后8周(實驗結束)IOP仍為造模前的2.5-3倍；補腎活血法對大鼠慢性EIOP模型LGN損傷的干預作用如下：輕度降低IOP；修復EIOP大鼠對LGN的損傷；提高EIOP大鼠LGN的BDNF表達。 結論：采用烙閉上鞏膜靜脈的方法建立慢性EIOP大鼠模型,操作簡單、效果穩(wěn)定,可獲得不少于兩個月的中等度EIOP;補腎活血法可以有效的保護慢性EIOP大鼠模型視功能,其作用主要表現(xiàn)在輕度降低IOP,修復EIOP大鼠對LGN的損傷,提高EIOP大鼠LGN的BDNF表達。
[Abstract]:Objective: to observe the effect of Bushen Huoxue method on (lateral geniculate nucleus,LGN injury of lateral geniculate body of chronic high intraocular pressure (elevated intraocular pressure, EIOP) model in rats, and to explore the mechanism of Bushen Huoxue method. Methods: chronic EIOP model of rats was established by cauterization of superior scleral vein and three branches of superior scleral vein of rats. The rats were randomly divided into three groups: blank group, model group and administration group. The rats were perfused continuously for 8 weeks and killed at the end of 8 weeks. The effects of Bushen Huoxue method on the pathological changes of intraocular pressure (intraocular pressure, IOP), LGN) and the expression of brain-derived neurotrophic factor (brain-derived neurotrophin factor, BDNF) in EIOP rats were observed. Results: the rat IOP was significantly increased by cauterization of scleral vein (P0.01), and the IOP was still 2.5-3 times as high as that before modeling at the end of the experiment at 8 weeks after the establishment of the model. The intervention effects of Bushen Huoxue method on LGN damage of chronic EIOP model in rats were as follows: mild decrease of LGN damage in EIOP rats repaired with IOP; and increase BDNF expression of LGN in EIOP rats. Conclusion: chronic EIOP rat model is established by cauterization of the superior scleral vein. The operation is simple, the effect is stable, and the moderate EIOP; of not less than two months can be obtained. Bushen Huoxue method can effectively protect the visual function of chronic EIOP rat model. The main effect of this method is to reduce the LGN damage caused by IOP, repair in EIOP rats and to increase the BDNF expression of LGN in EIOP rats.
【學位授予單位】：成都中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2010
【分類號】：R-332

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