發(fā)布時(shí)間：2018-11-26 19:41

【摘要】：第一部分酒精對(duì)雌鼠血清泌乳素水平及垂體濕重的影響 目的探討酒精對(duì)雌鼠血清泌乳素(PRL)水平、垂體濕重的影響,了解攝取酒精的濃度和年齡與血清PRL水平改變及垂體增生的相關(guān)性,了解飲酒與高泌乳素血癥(HP)的關(guān)系。 方法1.40只SD同齡成年雌鼠隨機(jī)分為4組,每組分別予10%,20%,30%酒精灌胃4周和8周各5只,對(duì)照組予等容積和時(shí)間蒸餾水灌胃；2.30只4周、6周、8周齡的SD雌鼠分別予以20%酒精灌胃4周,各自對(duì)照組均予等容積蒸餾水灌胃；3.各組大鼠處死后均用放射免疫分析法(RIA)檢測(cè)血清PRL水平,稱垂體濕重,分析攝取酒精的濃度和年齡與血清PRL水平、垂體濕重的相關(guān)性。 結(jié)果1.灌胃4周時(shí),30%酒精組大鼠血清PRL水平和垂體濕重明顯高于其它三組；灌胃8周時(shí),10%和20%酒精組明顯高于同期30%酒精組和對(duì)照組。組內(nèi)比較,灌胃8周時(shí),10%及20%酒精組大鼠PRL水平和垂體濕重均顯著高于灌胃4周時(shí),30%酒精組大鼠PRL水平和垂體濕重顯著低于4周時(shí),以上各差異均有統(tǒng)計(jì)學(xué)意義,P0.05。2.同濃度酒精灌胃4周后,各年齡組與相應(yīng)的對(duì)照組比較,4周齡大鼠血清PRL水平和垂體濕重顯著升高；6周齡和8周齡大鼠血清PRL水平、垂體濕重差異均無(wú)統(tǒng)計(jì)學(xué)意義,P0.05。 結(jié)論1.酒精攝入可引起雌鼠血清PRL水平發(fā)生改變,且有劑量依賴性和累積效應(yīng)：高濃度酒精可在較短時(shí)間內(nèi)使血清PRL水平升高,長(zhǎng)期低濃度酒精攝取可引起PRL水平顯著性增高。2.長(zhǎng)期大劑量酒精攝入可導(dǎo)致雌性大鼠垂體產(chǎn)生PRL的功能減退。3.較之成年期,幼年期雌性大鼠攝入酒精更易導(dǎo)致血清PRL水平升高。 第二部分?jǐn)z入酒精對(duì)孕鼠子代血清泌乳素及垂體濕重的影響 目的探討大鼠妊娠期大量攝入酒精對(duì)子代血清泌乳素水平、垂體濕重的影響。 方法6只孕鼠隨機(jī)分為2組,酒精組于懷孕第1天(P1)起給予20%酒精(2g/kg·d)灌胃至分娩前。對(duì)照組同期給予等量蒸餾水。子鼠喂養(yǎng)至4周后每組隨機(jī)抽取一半處死,余下的繼續(xù)喂養(yǎng)至8周處死。各組大鼠處死后均用放免法檢測(cè)血清泌乳素水平,并稱垂體濕重。 結(jié)果1.4周齡子鼠：酒精組的垂體濕重明顯高于對(duì)照組(P0.05),而兩組間PRL水平差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)；2.8周齡子鼠：酒精組的PRL水平和垂體濕重均較對(duì)照組顯著升高(P0.05)。 結(jié)論大鼠妊娠期大量攝入酒精可使子代血清泌乳素水平升高、垂體濕重增加。酒精可能通過(guò)刺激垂體增生,使得血泌乳素水平升高。 第三部分酒精攝入導(dǎo)致雌鼠血清泌乳素升高機(jī)制的探討 目的：探討酒精導(dǎo)致雌鼠血清泌乳素升高的機(jī)制。 方法：40只大鼠隨機(jī)分為2組,實(shí)驗(yàn)組給予20%酒精2m1灌胃,每日2次共8周,對(duì)照組給予等體積蒸餾水灌胃。檢測(cè)血清泌乳素水平、垂體濕重及免疫組化觀察垂體腺細(xì)胞增殖情況,庫(kù)倫陣列電化學(xué)高效液相色譜法檢測(cè)下丘腦勻漿去甲腎上腺素、多巴胺、5-羥色胺、r-氨基丁酸、谷氨酸水平并進(jìn)行比較。 結(jié)果：1.實(shí)驗(yàn)組血清泌乳素水平、垂體濕重均顯著高于對(duì)照組,P0.05。2.實(shí)驗(yàn)組下丘腦勻漿多巴胺、5-羥色胺、r-氨基丁酸顯著低于對(duì)照組,谷氨酸濃度顯著高于對(duì)照組, P0.05。去甲腎上腺素濃度與對(duì)照組比較無(wú)顯著性差異,P0.05。3.實(shí)驗(yàn)組垂體細(xì)胞Ki-67的表達(dá)顯著高于對(duì)照組,P0.05。 結(jié)論：酒精可以導(dǎo)致泌乳素升高,其機(jī)制可能為：1.垂體催乳細(xì)胞增殖；2.酒精刺激引起下丘腦泌乳素釋放抑制因子(PIF)如多巴胺、5-羥色胺、r-氨基丁酸等分泌減少,而泌乳素釋放因子(PRF)如谷氨酸分泌增多。
[Abstract]:The effect of first part of alcohol on serum prolactin level and pituitary-wet weight of female rats Objective To study the effect of alcohol on serum prolactin (PRL) level and pituitary wet weight in female rats, and to understand the concentration and age of intake alcohol and the changes of serum PRL level and the phase of pituitary hyperplasia. To understand the effect of drinking and hyperprolactinemia (HP) The adult female rats of the same age were randomly divided into 4 groups, each group was given 10%, 20%, 30% alcohol for 4 weeks and 8 weeks, and the control group was given equal volume and time distilled water. The control group was given equal volume distillation for 4 weeks. The serum PRL levels were detected by radioimmunoassay (RIA), and the concentration and age of the intake alcohol and the serum PRL level and the pituitary humidity were analyzed by radioimmunoassay (RIA). heavy correlation Results 1. The serum PRL level and the weight of the pituitary in the 30% alcohol group were significantly higher than those of the other three groups at 4 weeks. The 10% and 20% alcohol groups were significantly higher than that of the other three groups at 8 weeks. The levels of PRL in 10% and 20% alcohol group were significantly higher than that in the control group. The levels of PRL and the weight of the pituitary in the 30% alcohol group were significantly lower than that in the 4-week group. The serum PRL level and the pituitary-wet weight of the 4-week-old rats were significantly higher than those of the corresponding control group, and the serum PRL level and the pituitary-wet weight of the 6-week-old and 8-week-old rats were not statistically significant. Significance, P Conclusion 1. Alcohol intake can cause a change in serum PRL level in female rats with dose-dependent and cumulative effects: high-concentration alcohol can raise serum PRL level in a short time, and low-concentration alcohol intake can cause P A significant increase in the level of RL. 2. Long-term high-dose alcohol intake could lead to the pituitary production in female rats The function of the raw PRL is reduced. 3. Compared to the adult, the young female rats are more likely to take alcohol resulting in an elevated serum PRL level. The second part of the intake of alcohol to the pregnant rat Objective To study the effect of prolactinine and the wet weight of pituitary gland in the offspring of rats. Methods Six pregnant rats were randomly divided into two groups, and the alcohol group was given in the first day of pregnancy (P1). 20% alcohol (2g/ kg 路 d The same amount of distilled water was administered to the control group in the same period. The machine was killed by half, and the rest was continued for 8 weeks. All the rats were sacrificed. The serum prolactin level was detected by radioimmunoassay, and the pituitary-wet weight was weighed. The results were as follows: the weight of the pituitary in the alcohol group was significantly higher than that in the control group (P0.05), while the difference of the levels of PRL in the two groups was not significant (P0.05); and the PRL level in the 2. 8-week-old mice: the PRL of the alcohol group. The level and the wet weight of the pituitary gland were significantly higher than that in the control group (P0.05). A large amount of alcohol may increase the serum prolactin level in the offspring and the pituitary wet weight Increased. Alcohol may increase the level of prolactin by stimulating the pituitary gland. Increased. The third part of the alcohol intake resulted in the lactation of female rats Objective: To study the mechanism of the increase of serum prolactin in female rats by alcohol. Methods: 40 rats were randomly divided into 2 groups. The serum prolactin level, pituitary wet weight and immunohistochemistry were used to observe the proliferation of the pituitary gland cells, and the coulombic array electrochemical high performance liquid chromatography was used to detect the hypothalamus. Syrup of norepinephrine adenosine, dopamine, 5-hydroxytryptamine, r-aminobutyric acid, glutamate level and comparison. Results: 1. The serum prolactin level and the weight of the pituitary in the experimental group were significantly higher than that in the control group (P <0.05). The concentration of 5-hydroxytryptamine, r-aminobutyric acid was significantly lower than that of the control group, and the concentration of glutamic acid was significantly higher than that of the control group. The concentration of norepinephrine in the control group was no significant difference with the control group. The expression of Ki-67 in the pituitary cells of the experimental group It was significantly higher than that in the control group..................................................................................................................................................................................................
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2010
【分類(lèi)號(hào)】：R363

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 楊卉,林其德;高催乳素血癥的研究進(jìn)展[J];國(guó)外醫(yī)學(xué)(計(jì)劃生育分冊(cè));2004年03期

2 常青;應(yīng)用雌二醇替代治療的絕經(jīng)期婦女一次快速攝入乙醇后對(duì)泌乳素的影響[J];國(guó)外醫(yī)學(xué).婦產(chǎn)科學(xué)分冊(cè);1995年05期

3 劉長(zhǎng)云,盧映,楊利麗,杜玉華,張培森;酒精致雄性大鼠生殖細(xì)胞損傷的實(shí)驗(yàn)研究[J];中國(guó)計(jì)劃生育學(xué)雜志;2004年04期

4 楊曉明,崔慧先,薄愛(ài)華,石葛明;慢性酒精中毒大鼠腺垂體分泌細(xì)胞形態(tài)學(xué)改變的電鏡觀察[J];解剖科學(xué)進(jìn)展;2000年01期

5 崔慧先,楊曉明,康林,李雙成,石葛明;慢性酒精中毒對(duì)大鼠腺垂體LH細(xì)胞的影響[J];解剖科學(xué)進(jìn)展;2000年01期

6 項(xiàng)翠琴,汪根盛,傅慰祖,陳自強(qiáng);乙醇對(duì)大鼠腦紋狀體和海馬神經(jīng)遞質(zhì)的影響[J];環(huán)境與職業(yè)醫(yī)學(xué);2004年02期

7 王正朝;鐘靈秀;郭寧;張磊;石放雄;;慢性飲酒對(duì)小鼠卵巢功能及其對(duì)eCG反應(yīng)性的影響[J];南京農(nóng)業(yè)大學(xué)學(xué)報(bào);2008年03期

8 吳強(qiáng)恩,鄭力行,謝芳,吳同俊,阮國(guó)洪,周志俊;反相高效液相色譜熒光法測(cè)定大鼠腦組織中氨基酸類(lèi)神經(jīng)遞質(zhì)[J];復(fù)旦學(xué)報(bào)(醫(yī)學(xué)版);2005年03期

9 林金芳;;女性高泌乳素血癥診治的共識(shí)、爭(zhēng)議及循證研究方向[J];上海醫(yī)學(xué);2008年02期

10 高小玲,尹少甫,張本忠;乙醇、乙醛對(duì)紅細(xì)胞膜ATP酶活性影響的體外實(shí)驗(yàn)研究[J];中國(guó)工業(yè)醫(yī)學(xué)雜志;2004年04期

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