豚鼠左心室流出道慢反應(yīng)自律細(xì)胞分布區(qū)域的形態(tài)結(jié)構(gòu)
發(fā)布時(shí)間：2018-11-21 09:05
【摘要】： 心室流出道(左室的主動(dòng)脈前庭和右室的動(dòng)脈圓錐),一向被認(rèn)為僅是血液的輸出路徑,因而對(duì)其形態(tài)學(xué)和生理學(xué)特點(diǎn)一直缺乏詳細(xì)研究。我室近年來研究發(fā)現(xiàn),在豚鼠、兔及大鼠的主動(dòng)脈前庭的特定部位可記錄到自律性慢反應(yīng)動(dòng)作電位,表明在這一部位存在慢反應(yīng)自律細(xì)胞。這是在傳統(tǒng)的資料中未見記載的細(xì)胞的存在,提示心室流出道可能在心臟活動(dòng)過程中具有一定未知的功能。但以往的研究多是對(duì)這種電位的性質(zhì)、離子流基礎(chǔ)等方面的分析,而對(duì)產(chǎn)生這種電位的形態(tài)學(xué)基礎(chǔ)尚未見進(jìn)一步研究。為探討心室流出道組織的結(jié)構(gòu)特點(diǎn)和神經(jīng)分布,開展了本項(xiàng)工作。 在本實(shí)驗(yàn)中,利用常規(guī)細(xì)胞Qg玻璃微電極技術(shù),引導(dǎo)和分析豚鼠左心室流出道自發(fā)慢反應(yīng)電位,并在此基礎(chǔ)上,確定慢反應(yīng)自律細(xì)胞的分布區(qū)域,并將此區(qū)域制成形態(tài)學(xué)標(biāo)本,采用HE、Masson染色,鍍銀染色和透射電鏡技術(shù),對(duì)該區(qū)域的組織標(biāo)本,進(jìn)行了光鏡和電鏡觀察。重點(diǎn)是觀察該區(qū)域細(xì)胞的結(jié)構(gòu),膠原纖維、網(wǎng)狀纖維的分布,以確定慢反應(yīng)自律細(xì)胞分布的確切部位及這種細(xì)胞的超微結(jié)構(gòu)。為進(jìn)一ki從形態(tài)學(xué)探討這種自律細(xì)胞是否受自主神經(jīng)的調(diào)控,采用不同濃度的腎上腺素能和乙酰膽堿能激動(dòng)劑對(duì)該部位自發(fā)慢反應(yīng)電位影響,在此基礎(chǔ)上,通過免疫組織化學(xué)染色,觀察了神經(jīng)肽Y(neuropeptideY,NPY)能神經(jīng)纖維的分布,以判斷該區(qū)域是否接受自主神經(jīng)纖維的支配。 在大部分左心室流出道的標(biāo)本上可記錄到自發(fā)慢反應(yīng)電位,經(jīng)對(duì)這些標(biāo)本的形態(tài)學(xué)觀察發(fā)現(xiàn),在主動(dòng)脈瓣后瓣根部和心室肌的交界處,有一類似于月牙形的淡染區(qū)域。該區(qū)域細(xì)胞密集,可成團(tuán)或單獨(dú)存在,細(xì)胞形態(tài)類似P細(xì)胞樣細(xì)胞,可能就是慢反應(yīng)自律細(xì)胞的存在部位。細(xì)胞團(tuán)周圍含有豐富的膠原纖維和網(wǎng)狀纖維,對(duì)細(xì)胞團(tuán)的自律性電活動(dòng)可能起著絕緣和保護(hù)作用。 用交感神經(jīng)遞質(zhì)去甲腎上腺素(norepinephrine ,NE)和腎上腺素(epinephrine,E)灌流時(shí),可使自發(fā)放電頻率(rate of pacemakerfiring ,RPF),動(dòng)作電位幅值(amplitude of aetion potential,APA),4相自動(dòng)去極速率(veloeity of diastolic depolarization,VDD),0相最大除極速率(maximal rate of depolarization,Vmax)和最大舒張電位(maximal diastolic potential,MDP)明顯增加,但復(fù)極50%時(shí)間(50%of duration of action potential,APD50)和復(fù)極90%時(shí)間(90% of durationof action potential,APD90)變化不明顯,改用正常液沖洗后,上述指標(biāo)逐漸恢復(fù)。用副交感神經(jīng)遞質(zhì)(ACh)灌流時(shí),可使RPF,APA,VDD,Vmax和MDP絕對(duì)值明顯下降,APD50和APD90增加明顯,改用正常液沖洗后,上述指標(biāo)有不同程度的恢復(fù)。這些結(jié)果表明,自主神經(jīng)末稍的遞質(zhì)對(duì)該部慢反應(yīng)自律細(xì)胞的活動(dòng)有明顯作用。利用免疫組織化學(xué)染色觀察發(fā)現(xiàn),在包繞細(xì)胞團(tuán)周圍的結(jié)締組織中可見大量的NPY能陽(yáng)性神經(jīng)纖維呈線狀或束狀分布,在細(xì)胞團(tuán)內(nèi)也可偶見NPY陽(yáng)性神經(jīng)纖維。根據(jù)NPY是與NE共存于交感神經(jīng)末稍的遞質(zhì),表明產(chǎn)生慢反應(yīng)電位的細(xì)胞團(tuán)可能接受交感神經(jīng)纖維的調(diào)控。 在此實(shí)驗(yàn)中,我們發(fā)現(xiàn)心室流出道存在一特殊的細(xì)胞團(tuán)結(jié)構(gòu),該細(xì)胞團(tuán)可能是產(chǎn)生慢反應(yīng)動(dòng)作電位的自律細(xì)胞的存在部位;NPY能陽(yáng)性神經(jīng)纖維的存在,提示這些部位可能接受交感神經(jīng)的支配。
[Abstract]:The ventricular outflow tract (the arterial cone of the aortic vestibule and the right ventricle of the left chamber), which has been considered to be only the output path of the blood, has been lacking in a detailed study of its morphology and physiological characteristics. In recent years, I have found that a specific site in the aortic vestibule of guinea pigs, rabbits and rats can be recorded to a self-disciplined slow response action potential, indicating a slow response to self-regulation at this site. This is the absence of the cell described in the conventional data suggesting that the ventricular outflow tract may have an unknown function during cardiac activity. However, the previous studies have been made on the analysis of the properties of such potential and the basis of ion current, and the morphological basis for this potential has not yet been further studied. In order to study the structure and nerve distribution of the ventricular outflow tract, this work was carried out. In this experiment, the spontaneous slow reaction potential of the left ventricular outflow tract of the guinea pig was guided and analyzed by using the conventional cell Qg glass microelectrode technique, and on this basis, the distribution area of the slow-response self-regulation cell was determined, and the region was made into a morphological specimen, and HE and Mass were used. On the basis of the technique of dyeing, silver-plating and transmission electron microscopy, the tissue specimens of the region were examined and the light microscope and the light microscope were carried out. The focal point was to observe the structure of the cell, the distribution of the collagen fibers and the reticulation fibers to determine the exact location of the slow response self-regulation cell distribution and the cells. The ultrastructure of neuropeptide Y (neuroppt) was observed by immunohistochemical staining. ideY, NPY) the distribution of the nerve fibers to determine if the region is receptive to the autonomic fiber The control of the dimension. The spontaneous slow response potential can be recorded on the majority of the left ventricular outflow tract, and the morphology of these specimens has been found to be similar at the junction of the root of the aortic valve and the ventricular muscle. in a crescent-shaped light-dyed region. The region is dense, can be clustered or alone, and the cell morphology is similar to that of a P-cell-like cell, which may be a slow response. The presence of self-regulatory cells. There are abundant collagen fibers and reticular fibers around the cell mass, and the self-regulatory activity of the cell group may The spontaneous discharge frequency (RPF), the action potential amplitude value (APA), and the 4-phase autopolar rate (velocity of distaolic) can be achieved with the perfusion of the sympathomimetic norepinephrine (NE) and the epinephrine (E). The maximum depolarization rate (Vmax) and the maximum diastolic potential (MDP) of the 0-phase were significantly increased, but the time of 50% time (50% of duration of action, APD 50) and the time of the complex electrode (90% of time of action, APPD90) the change is not obvious, and the change is used instead The indexes of RPF, APA, VDD, Vmax, and MDP were significantly reduced after the normal fluid was flushed. The absolute values of RPF, APA, VDD, Vmax and MDP were significantly decreased with the perfusion of the parasympathetic nerve (ACh), and the APD50 and APPD90 increased significantly, and the normal liquid was used for flushing. The indicators showed a different degree of recovery, and the results showed that the end of the autonomic nerve was slower than that of the Department. It is found that a large number of NPY-positive nerve fibers in the connective tissue around the cell mass are linear or bundle-like in the connective tissue surrounding the cell mass, NPY-positive nerve fibers can also be seen occasionally. According to NPY, the cells that co-exist with the NE at the end of the sympathetic nerve indicate that the cells that produce the slow response potential It is possible to control the sympathetic fiber. In this experiment, we found that there was a special cell mass structure in the ventricular outflow tract, which could be the site of self-regulation of the slow response action potential, and the existence of NPY-positive nerve fibers.
【學(xué)位授予單位】：河北北方學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：R322.11
本文編號(hào)：2346512
[Abstract]:The ventricular outflow tract (the arterial cone of the aortic vestibule and the right ventricle of the left chamber), which has been considered to be only the output path of the blood, has been lacking in a detailed study of its morphology and physiological characteristics. In recent years, I have found that a specific site in the aortic vestibule of guinea pigs, rabbits and rats can be recorded to a self-disciplined slow response action potential, indicating a slow response to self-regulation at this site. This is the absence of the cell described in the conventional data suggesting that the ventricular outflow tract may have an unknown function during cardiac activity. However, the previous studies have been made on the analysis of the properties of such potential and the basis of ion current, and the morphological basis for this potential has not yet been further studied. In order to study the structure and nerve distribution of the ventricular outflow tract, this work was carried out. In this experiment, the spontaneous slow reaction potential of the left ventricular outflow tract of the guinea pig was guided and analyzed by using the conventional cell Qg glass microelectrode technique, and on this basis, the distribution area of the slow-response self-regulation cell was determined, and the region was made into a morphological specimen, and HE and Mass were used. On the basis of the technique of dyeing, silver-plating and transmission electron microscopy, the tissue specimens of the region were examined and the light microscope and the light microscope were carried out. The focal point was to observe the structure of the cell, the distribution of the collagen fibers and the reticulation fibers to determine the exact location of the slow response self-regulation cell distribution and the cells. The ultrastructure of neuropeptide Y (neuroppt) was observed by immunohistochemical staining. ideY, NPY) the distribution of the nerve fibers to determine if the region is receptive to the autonomic fiber The control of the dimension. The spontaneous slow response potential can be recorded on the majority of the left ventricular outflow tract, and the morphology of these specimens has been found to be similar at the junction of the root of the aortic valve and the ventricular muscle. in a crescent-shaped light-dyed region. The region is dense, can be clustered or alone, and the cell morphology is similar to that of a P-cell-like cell, which may be a slow response. The presence of self-regulatory cells. There are abundant collagen fibers and reticular fibers around the cell mass, and the self-regulatory activity of the cell group may The spontaneous discharge frequency (RPF), the action potential amplitude value (APA), and the 4-phase autopolar rate (velocity of distaolic) can be achieved with the perfusion of the sympathomimetic norepinephrine (NE) and the epinephrine (E). The maximum depolarization rate (Vmax) and the maximum diastolic potential (MDP) of the 0-phase were significantly increased, but the time of 50% time (50% of duration of action, APD 50) and the time of the complex electrode (90% of time of action, APPD90) the change is not obvious, and the change is used instead The indexes of RPF, APA, VDD, Vmax, and MDP were significantly reduced after the normal fluid was flushed. The absolute values of RPF, APA, VDD, Vmax and MDP were significantly decreased with the perfusion of the parasympathetic nerve (ACh), and the APD50 and APPD90 increased significantly, and the normal liquid was used for flushing. The indicators showed a different degree of recovery, and the results showed that the end of the autonomic nerve was slower than that of the Department. It is found that a large number of NPY-positive nerve fibers in the connective tissue around the cell mass are linear or bundle-like in the connective tissue surrounding the cell mass, NPY-positive nerve fibers can also be seen occasionally. According to NPY, the cells that co-exist with the NE at the end of the sympathetic nerve indicate that the cells that produce the slow response potential It is possible to control the sympathetic fiber. In this experiment, we found that there was a special cell mass structure in the ventricular outflow tract, which could be the site of self-regulation of the slow response action potential, and the existence of NPY-positive nerve fibers.
【學(xué)位授予單位】：河北北方學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：R322.11
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，本文編號(hào)：2346512
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