經(jīng)前期綜合征肝氣郁證模型大鼠不同腦區(qū)ERα、ERβ的分布與表達(dá)
發(fā)布時(shí)間：2018-11-18 17:04
【摘要】：目的：檢測(cè)經(jīng)前期綜合征(premenstrual syndrome,PMS)肝氣郁證模型大鼠海馬、下丘腦相關(guān)區(qū)域內(nèi)ERα和ERβ蛋白分布、表達(dá)的變化,初步探討PMS肝氣郁證發(fā)病的中樞機(jī)制,并研究中藥經(jīng)前舒顆粒對(duì)該病證的干預(yù)作用。 方法：以曠場(chǎng)實(shí)驗(yàn)、宏觀行為藥理學(xué)、陰道涂片法篩選合適大鼠進(jìn)入實(shí)驗(yàn),采用束縛造模法復(fù)制PMS肝氣郁證大鼠模型,以曠場(chǎng)實(shí)驗(yàn)結(jié)合宏觀行為藥理學(xué)評(píng)價(jià)模型,采用免疫組織化學(xué)技術(shù)ABC法檢測(cè)正常對(duì)照組、PMS肝氣郁模型組、模型給藥組大鼠海馬CA1、CA3、下丘腦腹內(nèi)側(cè)區(qū)(VMH)中ERα和ERβ的定位分布與積分光密度值(IOD),計(jì)算IOD(ERβ)/IOD(ERα). 結(jié)果：與正常組大鼠相比,模型組大鼠體重顯著減輕(P0.01),曠場(chǎng)實(shí)驗(yàn)水平得分、垂直得分、曠場(chǎng)實(shí)驗(yàn)總分均顯著減少(P0.01),大鼠表現(xiàn)出精神萎靡,眼神呆滯,對(duì)外界刺激不敏感的狀態(tài)；而造模并給予經(jīng)前舒顆粒的大鼠較模型組大鼠體重增長顯著(P0.05),曠場(chǎng)實(shí)驗(yàn)三項(xiàng)得分均顯著增加(P0.05或P0.01)。免疫組化結(jié)果顯示,三組大鼠海馬、下丘腦中ERα和ERβ的定位分布無顯著性差異。與正常組大鼠相比,模型組大鼠海馬CA1、CA3區(qū)錐體細(xì)胞出現(xiàn)形態(tài)變化,海馬CA1、CA3、下丘腦VMH區(qū)ERα和ERβIOD顯著升高(P0.05或P0.01),IOD(ERβ)/IOD(ERα)沒有顯著性差異。造模并給藥的大鼠海馬結(jié)構(gòu)無異常；與模型組相比,海馬CA1、CA3、下丘腦VMH區(qū)ERα和ERβIOD顯著降低(P0.01),下丘腦VMH區(qū)IOD(ERβ)/IOD(ERα)顯著下降(P0.05)。 結(jié)論：采用束縛造模法可以成功制備PMS肝氣郁證大鼠模型,曠場(chǎng)實(shí)驗(yàn)結(jié)合宏觀行為學(xué)可有效評(píng)價(jià)模型。大鼠海馬CA1、CA3、下丘腦VMH區(qū)ERα、ERβ蛋白表達(dá)升高可能與PMS肝氣郁證關(guān)系密切,可能是PMS肝氣郁證發(fā)病的中樞機(jī)制之一；中藥經(jīng)前舒顆�？梢杂行Ъm正模型大鼠上述腦區(qū)ERα、ERβ蛋白表達(dá)異常升高的狀態(tài),同時(shí)下調(diào)下丘腦VMH區(qū)ERβ/ERα,可能是該藥治療PMS肝氣郁證的機(jī)制之一。
[Abstract]:Objective: to investigate the distribution and expression of ER 偽 and ER 尾 protein in hippocampus and hypothalamus of rats with liver qi depression syndrome (premenstrual syndrome,PMS), and to explore the central mechanism of PMS liver qi depression syndrome. And to study the intervention effect of Jingqianshu granule on the syndrome of the disease. Methods: open field experiment, macroscopic behavior pharmacology and vaginal smear were used to screen suitable rats to enter the experiment. The rat model of PMS liver qi depression was established by binding method. The open field experiment combined with macroscopic behavior pharmacological evaluation model was used. Localization and distribution of ER 偽 and ER 尾 in (VMH) of hypothalamic ventromedial area of hippocampus CA1,CA3, in rats of normal control group, PMS liver qi depression model group and model group by immunohistochemical technique ABC method and integrated optical density (IOD),) IOD (ER 尾 / IOD (ER 偽). Results: compared with the normal group, the rats in the model group were significantly reduced in body weight (P0.01), open field test level score, vertical score and total open field test score (P0.01). The state of being insensitive to external stimuli; Compared with the model group, the weight of the rats in the model group increased significantly (P0.05), and the scores of the three items in the open field test were significantly increased (P0.05 or P0.01). Immunohistochemical results showed that the localization of ER 偽 and ER 尾 in hippocampus and hypothalamus of the three groups had no significant difference. Compared with the normal group, the hippocampal CA1,CA3 pyramidal cells in the model group showed morphological changes, but ER 偽 and ER 尾 IOD in the VMH area of the hypothalamus of hippocampus CA1,CA3, were significantly increased (P0.05 or P0.01), IOD (ER 尾 / IOD (ER 偽). Compared with the model group, the ER 偽 and ER 尾 IOD in the hypothalamic VMH area of hippocampus CA1,CA3, decreased significantly (P0.01), and IOD (ER 尾 / IOD (ER 偽 in the VMH area of the hypothalamus decreased significantly (P0.05). Conclusion: the rat model of PMS liver qi depression can be successfully established by using the method of restraint. Open field experiment combined with macroscopical behavior can effectively evaluate the model. The increased expression of ER 偽 and ER 尾 in the hypothalamus of CA1,CA3, may be closely related to the stagnation of liver qi of PMS, and may be one of the central mechanisms of PMS syndrome of liver qi depression. Jingqianshu granule can effectively correct the abnormal increase of ER 偽 and ER 尾 protein expression in the above-mentioned brain regions of the model rats, and down-regulate ER 尾 / ER 偽 in the hypothalamic VMH area, which may be one of the mechanisms of the treatment of PMS liver qi stagnation syndrome.
【學(xué)位授予單位】：山東中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：R-332
本文編號(hào)：2340609
[Abstract]:Objective: to investigate the distribution and expression of ER 偽 and ER 尾 protein in hippocampus and hypothalamus of rats with liver qi depression syndrome (premenstrual syndrome,PMS), and to explore the central mechanism of PMS liver qi depression syndrome. And to study the intervention effect of Jingqianshu granule on the syndrome of the disease. Methods: open field experiment, macroscopic behavior pharmacology and vaginal smear were used to screen suitable rats to enter the experiment. The rat model of PMS liver qi depression was established by binding method. The open field experiment combined with macroscopic behavior pharmacological evaluation model was used. Localization and distribution of ER 偽 and ER 尾 in (VMH) of hypothalamic ventromedial area of hippocampus CA1,CA3, in rats of normal control group, PMS liver qi depression model group and model group by immunohistochemical technique ABC method and integrated optical density (IOD),) IOD (ER 尾 / IOD (ER 偽). Results: compared with the normal group, the rats in the model group were significantly reduced in body weight (P0.01), open field test level score, vertical score and total open field test score (P0.01). The state of being insensitive to external stimuli; Compared with the model group, the weight of the rats in the model group increased significantly (P0.05), and the scores of the three items in the open field test were significantly increased (P0.05 or P0.01). Immunohistochemical results showed that the localization of ER 偽 and ER 尾 in hippocampus and hypothalamus of the three groups had no significant difference. Compared with the normal group, the hippocampal CA1,CA3 pyramidal cells in the model group showed morphological changes, but ER 偽 and ER 尾 IOD in the VMH area of the hypothalamus of hippocampus CA1,CA3, were significantly increased (P0.05 or P0.01), IOD (ER 尾 / IOD (ER 偽). Compared with the model group, the ER 偽 and ER 尾 IOD in the hypothalamic VMH area of hippocampus CA1,CA3, decreased significantly (P0.01), and IOD (ER 尾 / IOD (ER 偽 in the VMH area of the hypothalamus decreased significantly (P0.05). Conclusion: the rat model of PMS liver qi depression can be successfully established by using the method of restraint. Open field experiment combined with macroscopical behavior can effectively evaluate the model. The increased expression of ER 偽 and ER 尾 in the hypothalamus of CA1,CA3, may be closely related to the stagnation of liver qi of PMS, and may be one of the central mechanisms of PMS syndrome of liver qi depression. Jingqianshu granule can effectively correct the abnormal increase of ER 偽 and ER 尾 protein expression in the above-mentioned brain regions of the model rats, and down-regulate ER 尾 / ER 偽 in the hypothalamic VMH area, which may be one of the mechanisms of the treatment of PMS liver qi stagnation syndrome.
【學(xué)位授予單位】：山東中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：R-332
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