【摘要】：目的了解外周血淋巴細(xì)胞微核標(biāo)本制片的影響因素,尋找出最佳的室內(nèi)質(zhì)量控制制作條件。方法比較制片過程中不同的接種血量、培養(yǎng)溫度、低滲時(shí)間以及離心速度對(duì)外周血中微核標(biāo)本制作的影響,尋找最佳的室內(nèi)質(zhì)量控制制片效果。結(jié)果外周血微核制片過程中最佳的室內(nèi)質(zhì)量控制條件是接種血量是0.7×32規(guī)格注射器針頭20~25滴;培養(yǎng)溫度應(yīng)該嚴(yán)格控制在(37.0±0.5)℃;最佳低滲時(shí)間是6~9min;最佳離心速度1500~2000 r/min;固定液應(yīng)在使用前臨時(shí)配置,放置1 h應(yīng)廢棄;玻片冷藏處理的效果比熱水處理后的較好。結(jié)論外周血淋巴細(xì)胞微核標(biāo)本制片的影響因素有接種血量、培養(yǎng)溫度、低滲時(shí)間以及離心速度等,相關(guān)實(shí)驗(yàn)室予以關(guān)注,以確保其制備質(zhì)量,提高微核檢出準(zhǔn)確率和讀片效率,及時(shí)準(zhǔn)確反映輻射損傷情況,對(duì)輻射防護(hù)作出正確的評(píng)價(jià)。
[Abstract]:Objective to investigate the influencing factors of micronucleus preparation in peripheral blood lymphocytes and to find out the best indoor quality control conditions. Methods the effects of different inoculation blood volume, culture temperature, low osmotic time and centrifugation speed on the preparation of micronucleus samples in peripheral blood were compared in order to find out the best effect of indoor quality control. Results the optimal indoor quality control conditions during the preparation of peripheral blood micronucleus were as follows: blood volume of 0.7 脳 32 standard syringe needle 2025 drops, culture temperature strictly controlled at (37.0 鹵0.5) 鈩，

本文編號(hào)：2338998