【摘要】：目的了解外周血淋巴細胞微核標本制片的影響因素,尋找出最佳的室內質量控制制作條件。方法比較制片過程中不同的接種血量、培養(yǎng)溫度、低滲時間以及離心速度對外周血中微核標本制作的影響,尋找最佳的室內質量控制制片效果。結果外周血微核制片過程中最佳的室內質量控制條件是接種血量是0.7×32規(guī)格注射器針頭20~25滴;培養(yǎng)溫度應該嚴格控制在(37.0±0.5)℃;最佳低滲時間是6~9min;最佳離心速度1500~2000 r/min;固定液應在使用前臨時配置,放置1 h應廢棄;玻片冷藏處理的效果比熱水處理后的較好。結論外周血淋巴細胞微核標本制片的影響因素有接種血量、培養(yǎng)溫度、低滲時間以及離心速度等,相關實驗室予以關注,以確保其制備質量,提高微核檢出準確率和讀片效率,及時準確反映輻射損傷情況,對輻射防護作出正確的評價。
[Abstract]:Objective to investigate the influencing factors of micronucleus preparation in peripheral blood lymphocytes and to find out the best indoor quality control conditions. Methods the effects of different inoculation blood volume, culture temperature, low osmotic time and centrifugation speed on the preparation of micronucleus samples in peripheral blood were compared in order to find out the best effect of indoor quality control. Results the optimal indoor quality control conditions during the preparation of peripheral blood micronucleus were as follows: blood volume of 0.7 脳 32 standard syringe needle 2025 drops, culture temperature strictly controlled at (37.0 鹵0.5) 鈩，

本文編號：2338998