【摘要】：目的:研究糖尿病大鼠模型的建立及其蛋白組學的變化,從蛋白質(zhì)的分子水平上探索糖尿病發(fā)生的病理生理機制。方法:用高脂高糖飲食+鏈脲佐菌素的方法建立糖尿病大鼠模型,采用同位素標記技術(shù),利用高效液相色譜儀及四級桿-靜電場軌道阱質(zhì)譜儀,對糖尿病大鼠進行串級質(zhì)譜數(shù)據(jù)分析,對檢測出的有統(tǒng)計學意義的表達差異蛋白進行研究。結(jié)果:第2周開始,模型組與正常組的血糖差異有統(tǒng)計學意義(P0.05)。第10周,模型組血糖基本達到了糖尿病模型的標準,空腹血糖16.7 mmol/L;蛋白組學檢測共獲得有顯著差異的蛋白50個,其中上調(diào)大于1.5倍的蛋白17個,主要有MHCⅡ反式激活因子、主要尿蛋白、LIM結(jié)構(gòu)域蛋白等;下調(diào)小于0.75倍的50個,主要有肉堿乙酰轉(zhuǎn)移酶、多聚免疫球蛋白受體前體等。結(jié)論:建立了相對符合糖尿病發(fā)生發(fā)展規(guī)律的糖尿病大鼠模型,血清蛋白組學分析糖尿病發(fā)生的機制中存在免疫系統(tǒng)、糖代謝、脂代謝紊亂,從蛋白分子的水平研究糖尿病發(fā)生的病理生理基礎(chǔ),具有重要學術(shù)和臨床意義。
[Abstract]:Aim: to study the establishment of diabetic rat model and its proteomic changes, and to explore the pathophysiological mechanism of diabetes mellitus at the molecular level. Methods: the diabetic rat model was established by using high fat and high sugar diet streptozotocin. High performance liquid chromatography (HPLC) and quaternary bar electrostatic field trap mass spectrometer were used. The differentially expressed proteins in diabetic rats were analyzed by cascade mass spectrometry. Results: there was significant difference in blood glucose between the model group and the normal group at the second week (P0.05). In the 10th week, the blood glucose of the model group basically reached the standard of diabetic model, fasting blood glucose 16. 7 mmol/L; A total of 50 proteins with significant difference were obtained by proteomics analysis, 17 of which were up-regulated more than 1.5 times, mainly MHC 鈪，

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