【摘要】： 股骨頭缺血性壞死是臨床常見病、多發(fā)病,治療上除手術(shù)外都是基于改善股骨頭周圍血液循環(huán)。骨髓間充質(zhì)干細胞取材方便,能在體外大量擴增,具有多種分化潛能,來源于自體骨髓的細胞移植時不存在免疫排斥,同種異體的間充質(zhì)干細胞免疫原性很低,為組織損傷修復提供了新的種子細胞。 本研究采用密度梯度離心分離法對rMSCs進行分離擴增,通過形態(tài)學和成骨、成脂方向誘導分化,證實分離得到的細胞為骨髓間充質(zhì)干細胞。采用菲立磁-多聚賴氨酸對得到的rMSCs進行磁標記,標記率為100%;用1.5TMRI對標記細胞進行了體外成像研究,表明GRE T2*WI序列可以作為磁標記細胞研究的首選序列。通過注射馬血清及甲潑尼龍的方法建立了兔激素性股骨頭缺血壞死模型,該模型骨壞死處于0～I期,是研究早期骨壞死的理想模型。采用介入導管技術(shù)將骨髓間充質(zhì)干細胞移植到兔股骨頭缺血壞死模型體內(nèi),顯示移植后病變部位可見明顯骨修復,移植細胞可以在病變局部存活。在此基礎(chǔ)上采用全骨髓培養(yǎng)法對胎兒fMSCs進行分離和擴增,并進行fMSCs磁標記成像,獲得了與兔MSCs相似的結(jié)果。本研究從兔骨髓間充質(zhì)干細胞研究獲得經(jīng)驗并逐步應(yīng)用到人骨髓間充質(zhì)干細胞研究上,期望為進一步的骨髓間充質(zhì)干細胞移植治療股骨頭缺血壞死的臨床應(yīng)用做些有益的探索。
[Abstract]:Avascular necrosis of the femoral head is a common clinical disease. The treatment of avascular necrosis of femoral head is based on the improvement of peripheral blood circulation except surgery. Bone marrow mesenchymal stem cells (BMSCs) are easy to take, can be expanded in vitro, and have a variety of differentiation potential. There is no immune rejection in transplantation of cells derived from autologous bone marrow, and the immunogenicity of allogeneic mesenchymal stem cells is very low. It provides a new seed cell for tissue damage repair. In this study, rMSCs was isolated and amplified by density gradient centrifugation. The cells were identified as bone marrow mesenchymal stem cells (BMSCs) by morphology and osteogenesis. The labeled rMSCs was labeled with phenidomagnetic poly-lysine and the labeling rate was 100. The in vitro imaging of labeled cells with 1.5TMRI showed that the GRE T2*WI sequence could be the preferred sequence in the study of magnetically labeled cells. A rabbit model of steroid-induced avascular necrosis of femoral head was established by injecting horse serum and methylprednisolone. Bone marrow mesenchymal stem cells (BMSCs) were transplanted into rabbit model of avascular necrosis of femoral head by interventional catheter technique. On this basis, fetal fMSCs was isolated and amplified by whole bone marrow culture method, and fMSCs magnetic labeling imaging was performed. The results were similar to those of rabbit MSCs. In this study, we obtained experience from rabbit bone marrow mesenchymal stem cell research and applied it to human bone marrow mesenchymal stem cell research step by step, hoping to make some useful exploration for the further clinical application of bone marrow mesenchymal stem cell transplantation in the treatment of avascular necrosis of femoral head.
【學位授予單位】：吉林大學
【學位級別】：博士
【學位授予年份】：2009
【分類號】：R329

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