【摘要】： 目的:通過觀察外源性溶血磷脂酸(LPA)對缺血缺氧狀態(tài)下豚鼠心室肌細胞動作電位及肌纖維膜上ATP敏感鉀通道電流,豚鼠心室肌細胞線粒體膜電位的影響,探討LPA對缺血缺氧心肌細胞的作用及其機理。 方法:應用全細胞膜片鉗技術,觀察LPA對缺血缺氧狀態(tài)下豚鼠心室肌細胞動作電位的影響;觀察LPA對缺血缺氧狀態(tài)下豚鼠心室肌細胞肌纖維膜上ATP敏感鉀通道電流的影響;采用細胞培養(yǎng)和流式細胞技術,觀察LPA對缺血缺氧豚鼠心室肌細胞線粒體膜電位的影響。 結果:外源性LPA可以縮短缺血缺氧豚鼠心室肌細胞動作電位時程,增強缺血缺氧豚鼠心室肌細胞肌纖維膜上ATP敏感鉀通道的電流幅度,并且升高缺血缺氧的豚鼠心室肌細胞線粒體膜電位。 結論:外源性LPA通過增加缺血缺氧豚鼠心室肌細胞肌纖維膜上ATP敏感鉀通道電流,縮短動作電位時程,減少鈣離子內流,降低細胞內游離鈣濃度,保護缺血缺氧心肌細胞;同時外源性LPA可以升高缺血缺氧豚鼠心室肌細胞線粒體膜電位,防止線粒體內鈣超載,保護缺血缺氧心肌細胞。 創(chuàng)新點: 1.外源性LPA縮短缺血缺氧豚鼠心室肌細胞動作電位時程。 2.外源性LPA增強缺血缺氧豚鼠心室肌細胞肌纖維膜上ATP敏感鉀通道電流幅度。 3.外源性LPA提高缺血缺氧豚鼠心室肌細胞線粒體膜電位。
[Abstract]:Aim: to observe the effects of exogenous lysophosphatidic acid (LPA) on action potential of guinea pig ventricular myocytes, ATP sensitive potassium channel current on myofilm and mitochondrial membrane potential of guinea pig ventricular myocytes under ischemia and hypoxia. To investigate the effect and mechanism of LPA on ischemic and hypoxic cardiomyocytes. Methods: whole cell patch clamp technique was used to observe the effect of LPA on the action potential of guinea pig ventricular myocytes under ischemia and hypoxia, and to observe the effect of LPA on the current of ATP sensitive potassium channel in the myocyte membrane of guinea pig ventricular myocytes under the condition of ischemia and hypoxia. Cell culture and flow cytometry were used to observe the effect of LPA on mitochondrial membrane potential in guinea pig ventricular myocytes after ischemia and hypoxia. Results: exogenous LPA could shorten the duration of action potential of guinea pig ventricular myocytes after ischemia and hypoxia, and enhance the current amplitude of ATP sensitive potassium channel on the membrane of ventricular myocytes of ischemic hypoxia guinea pig ventricular myocytes. The mitochondrial membrane potential of guinea pig ventricular myocytes with ischemia and hypoxia was increased. Conclusion: exogenous LPA can shorten the duration of action potential, decrease the influx of calcium ion, decrease the concentration of intracellular free calcium and protect myocardial cells from ischemia and hypoxia by increasing the current of ATP sensitive potassium channel on the myocyte membrane of guinea pig ventricular myocytes after ischemia and hypoxia. At the same time, exogenous LPA could increase mitochondrial membrane potential of ventricular myocytes from ischemia and hypoxia, prevent calcium overload in mitochondria and protect myocardial cells from ischemia and hypoxia. Innovation: 1. Exogenous LPA shortens the duration of action potential in guinea pig ventricular myocytes after ischemia and hypoxia. 2. Exogenous LPA enhanced the current amplitude of ATP sensitive potassium channel in myocyte membrane of guinea pig ventricular myocytes after ischemia and hypoxia. 3. Exogenous LPA enhances mitochondrial membrane potential in guinea pig ventricular myocytes after ischemia and hypoxia.
【學位授予單位】：吉林大學
【學位級別】：碩士
【學位授予年份】：2009
【分類號】：R363

【參考文獻】

相關期刊論文 前4條

1 任永生,潘春水,伍期專,龐永政,唐朝樞,齊永芬;內皮素參與溶血磷脂酸促血管平滑肌細胞的增殖[J];北京大學學報(醫(yī)學版);2003年05期

2 陳曦;;溶血磷脂酸受體及下游信號通路在心臟細胞生長中的調節(jié)作用[J];生理學報;2007年05期

3 羅浩;江洪;曾彬;蔡軍;王騰;;溶血磷脂酸對胚胎干細胞分化心肌細胞L型鈣通道電流的影響[J];中國心臟起搏與心電生理雜志;2006年05期

4 趙智明;蔡輝;郭郡浩;;溶血磷脂酸對新生大鼠心肌成纖維細胞分泌NO、ET和TGFβ1的影響[J];中國微循環(huán);2006年03期

，

本文編號：2314681