【摘要】： 腎上腺素(EP)是哺乳動(dòng)物和人類的一種重要的神經(jīng)遞質(zhì),具有重要的生理功能,分析測(cè)定人體內(nèi)腎上腺素的含量具有重要的意義。實(shí)驗(yàn)研究表明,導(dǎo)電聚合物聚苯胺(PAn)具有比表面積高、環(huán)境穩(wěn)定性良好以及導(dǎo)電率高等特征,將其作為載體材料,沉積、分散具有催化活性的Pt金屬,制備出分散鉑聚苯胺修飾電極(Pt/PAn/GC)。本實(shí)驗(yàn)主要研究了Pt/PAn/GC電極對(duì)腎上腺素的催化作用以及影響該修飾電極催化活性的制備因素。 本文選擇EP為探針,分析影響鉑在聚苯胺膜上電沉積的因素以及聚苯胺膜的厚度對(duì)修飾電極催化性能的影響,確定沉積鉑的最佳條件為：摻雜酸為鹽酸,其濃度為2.0mol／L、掃速50mV／s。采用循環(huán)伏安法研究了EP在分散鉑聚苯胺修飾玻碳電極上pH=0.5的HCl介質(zhì)、HClO4介質(zhì)、H3PO4介質(zhì)、HNO3介質(zhì)、H2SO4介質(zhì)、鹽酸-氯化鉀溶液、高氯酸-高氯酸鈉溶液和硝酸-硝酸鉀溶液中的電化學(xué)行為。實(shí)驗(yàn)結(jié)果表明,通過循環(huán)伏安掃描法制備的Pt/PAn/GC電極對(duì)腎上腺素有較好的催化性,與聚苯胺(PAn/GC)修飾電極和裸玻碳電極(GCE)相比,Pt/PAn/GC電極對(duì)EP具有更好的催化能力。通過改變不同的掃描速度,確定了掃速和氧化峰電流(ipa)的線性關(guān)系,說明腎上腺素在分散鉑聚苯胺修飾電極上的電子轉(zhuǎn)移過程受擴(kuò)散控制。在1.5 mol/L的鹽酸-氯化鉀溶液中,氧化峰電流與EP的濃度在9.0×10-6 mol/L～2.5×10-3 mol/L范圍內(nèi)呈良好的線性關(guān)系,檢出限為1.6×10-6 mol/L�？捎糜趯�(shí)際樣品中EP的測(cè)定。 采用交流阻抗法研究了分散鉑聚苯胺修飾電極在鹽酸溶液和鹽酸-氯化鉀溶液中的電化學(xué)行為。實(shí)驗(yàn)結(jié)果表明,隨鹽酸溶液濃度的增加,聚苯胺膜的電荷傳遞電阻越來越小,電荷的傳遞速度加快；研究了分散鉑聚苯胺修飾電極、聚苯胺修飾電極和玻碳電極在鹽酸-氯化鉀溶液中的阻抗性能,結(jié)果表明,EP在聚苯胺修飾電極上的電氧化電阻比分散鉑聚苯胺修飾電極上的大,分散鉑聚苯胺修飾的電極可降低電極上EP氧化的電化學(xué)極化電阻。這與前面循環(huán)伏安法測(cè)得的結(jié)果一致。
[Abstract]:Epinephrine (EP) is an important neurotransmitter in mammals and humans and has important physiological functions. It is of great significance to analyze and determine the content of epinephrine in human body. The experimental results show that the conductive polymer Polyaniline (PAn) has the characteristics of high specific surface area, good environmental stability and high conductivity. It is used as the carrier material to deposit and disperse the Pt metal with catalytic activity. The dispersed platinum Polyaniline modified electrode (Pt/PAn/GC) was prepared. The catalytic effect of Pt/PAn/GC electrode on epinephrine and the preparation factors affecting the catalytic activity of the modified electrode were studied. In this paper, using EP as probe, the factors affecting the electrodeposition of platinum on Polyaniline film and the effect of the thickness of Polyaniline film on the catalytic performance of modified electrode are analyzed. The optimum conditions for platinum deposition are as follows: the doping acid is hydrochloric acid, the concentration is 2.0 mol / L, Sweep speed 50 MV / s. Cyclic voltammetry was used to study the HCl medium, HClO4 medium, H3PO4 medium, HNO3 medium, H2SO4 medium, hydrochloric acid-potassium chloride solution of pH=0.5 on the dispersible platinum Polyaniline modified glassy carbon electrode by cyclic voltammetry. Electrochemical behavior of perchloric acid-sodium perchlorate solution and nitric acid-potassium nitrate solution. The experimental results show that the Pt/PAn/GC electrode prepared by cyclic voltammetry has a good catalytic activity to epinephrine, compared with the (GCE) electrode modified by Polyaniline (PAn/GC) and the bare glassy carbon electrode. Pt/PAn/GC electrode has better catalytic ability to EP. The linear relationship between the scanning speed and the oxidation peak current (ipa) was determined by changing the different scanning rates. It was concluded that the electron transfer process of epinephrine at the dispersed platinum Polyaniline modified electrode was controlled by diffusion. In 1.5 mol/L hydrochloric acid-potassium chloride solution, the oxidation peak current showed a good linear relationship with the concentration of EP in the range of 9.0 脳 10-6 mol/L~2.5 脳 10-3 mol/L, and the detection limit was 1.6 脳 10-6 mol/L.. It can be used for the determination of EP in actual samples. The electrochemical behavior of dispersed platinum Polyaniline modified electrode in hydrochloric acid solution and hydrochloric acid-potassium chloride solution was studied by AC impedance method. The experimental results show that the charge transfer resistance of Polyaniline film decreases with the concentration of hydrochloric acid solution increasing, and the charge transfer rate increases. The impedance properties of dispersed platinum Polyaniline modified electrode, Polyaniline modified electrode and glassy carbon electrode in hydrochloric acid-potassium chloride solution were studied. The electrooxidation resistance of EP on Polyaniline modified electrode is larger than that on dispersed Platinum Polyaniline modified electrode, and the electrochemical polarization resistance of EP oxidation on the modified electrode can be reduced by dispersed platinum Polyaniline modified electrode. This is consistent with the results obtained by the previous cyclic voltammetry.
【學(xué)位授予單位】：曲阜師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：R341

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