單純皰疹病毒Ⅰ型糖蛋白B基因疫苗安全性的初步研究
發(fā)布時(shí)間：2018-11-05 16:55
【摘要】： 目的:初步檢驗(yàn)單純皰疹病毒Ⅰ型糖蛋白B基因疫苗對(duì)小鼠的安全性。 方法:隨機(jī)將昆明小鼠分為4組,即對(duì)照組、100μg組,300μg組,500μg組,每組6只,雌雄各半。各組分別股四頭肌肌肉注射1ml生理鹽水、100μg/ml、300μg/ml、500μg/ml pcDNA3-gB,每10天免疫一次,共3次。末次免疫10天后,留取1ml凝血和20ul抗凝血各一份進(jìn)行血液生理學(xué)、血液生化學(xué)檢測(cè),并對(duì)心、肝、脾、腎、腦、角膜、視網(wǎng)膜和三叉神經(jīng)節(jié)8個(gè)組織器官進(jìn)行病理切片檢查及基因整合情況的檢測(cè)。 結(jié)果:免疫不同劑量單純皰疹病毒Ⅰ型糖蛋白B基因疫苗的小鼠全部存活,并且其血液生理學(xué)、血液生化學(xué)指標(biāo)、各組織器官的病理切片結(jié)果及基因整合情況與對(duì)照組小鼠的檢測(cè)結(jié)果未見明顯差異(P0.05),不具有統(tǒng)計(jì)學(xué)意義。 結(jié)論:初步說(shuō)明單純皰疹病毒Ⅰ型糖蛋白B基因疫苗無(wú)病毒致病性,對(duì)小鼠是安全的,為下一步應(yīng)用于大型實(shí)驗(yàn)動(dòng)物及臨床奠定了基礎(chǔ)。
[Abstract]:Objective: to study the safety of herpes simplex virus type I glycoprotein B gene vaccine in mice. Methods: Kunming mice were randomly divided into four groups: control group, 100 渭 g group, 300 渭 g group and 500 渭 g group. The quadriceps femoris were intramuscularly injected with 1ml saline, 100 渭 g / ml, 300 渭 g / ml, 500 渭 g/ml pcDNA3-gB, once every 10 days, for 3 times. Ten days after the last immunization, blood physiology, blood biochemistry, heart, liver, spleen, kidney, brain, cornea were detected by 1ml coagulation and 20ul anticoagulant. Eight tissues and organs of retina and trigeminal ganglion were examined by pathological section and gene integration. Results: all the mice immunized with different doses of herpes simplex virus glycoprotein B gene vaccine survived, and their blood physiology, blood biochemistry index, The results of pathological sections and gene integration of different tissues and organs were not significantly different from those of control mice (P0.05). Conclusion: herpes simplex virus type I glycoprotein B gene vaccine has no virulence and is safe for mice. It lays a foundation for further application in large laboratory animals and clinic.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2008
【分類號(hào)】：R3921
本文編號(hào)：2312705
[Abstract]:Objective: to study the safety of herpes simplex virus type I glycoprotein B gene vaccine in mice. Methods: Kunming mice were randomly divided into four groups: control group, 100 渭 g group, 300 渭 g group and 500 渭 g group. The quadriceps femoris were intramuscularly injected with 1ml saline, 100 渭 g / ml, 300 渭 g / ml, 500 渭 g/ml pcDNA3-gB, once every 10 days, for 3 times. Ten days after the last immunization, blood physiology, blood biochemistry, heart, liver, spleen, kidney, brain, cornea were detected by 1ml coagulation and 20ul anticoagulant. Eight tissues and organs of retina and trigeminal ganglion were examined by pathological section and gene integration. Results: all the mice immunized with different doses of herpes simplex virus glycoprotein B gene vaccine survived, and their blood physiology, blood biochemistry index, The results of pathological sections and gene integration of different tissues and organs were not significantly different from those of control mice (P0.05). Conclusion: herpes simplex virus type I glycoprotein B gene vaccine has no virulence and is safe for mice. It lays a foundation for further application in large laboratory animals and clinic.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2008
【分類號(hào)】：R3921
【引證文獻(xiàn)】
相關(guān)碩士學(xué)位論文 前1條
1 劉欣;生長(zhǎng)抑素DNA免疫對(duì)小鼠體組織的影響及表達(dá)產(chǎn)物的分布規(guī)律[D];華中農(nóng)業(yè)大學(xué);2010年
，本文編號(hào)：2312705
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