【摘要】：目的:探索大鼠海馬神經(jīng)干細(xì)胞離體培養(yǎng)的實(shí)驗(yàn)條件和方法,并建立一種簡便且獲得率較高的實(shí)驗(yàn)方法。方法:分別隨機(jī)選取新生1~3 d SD大鼠和孕18~19 d SD胎鼠,并提取海馬組織,采用機(jī)械吹打結(jié)合胰蛋白酶消化的方法獲得單細(xì)胞懸液,然后對(duì)培養(yǎng)出的細(xì)胞進(jìn)行鑒定和誘導(dǎo)分化,最后測定并分別比較兩組原代細(xì)胞獲得率和增殖率以及凋亡率之間的差異。結(jié)果:來源于兩種不同發(fā)育階段大鼠的海馬組織培養(yǎng)出的細(xì)胞均呈Nestin陽性和Brd U陽性的神經(jīng)球樣結(jié)構(gòu),誘導(dǎo)分化后的細(xì)胞高表達(dá)βⅢ-tublin和GFAP。其中,胎鼠組原代細(xì)胞獲得率較高、增殖能力較強(qiáng),并有統(tǒng)計(jì)學(xué)差異(P0.05);但兩組神經(jīng)干細(xì)胞的凋亡率無明顯差異(P0.05)。結(jié)論:胎鼠和新生鼠海馬組織均能培養(yǎng)出神經(jīng)干細(xì)胞,而且胎鼠組細(xì)胞產(chǎn)出率更高。
[Abstract]:Aim: to explore the experimental conditions and methods of rat hippocampal neural stem cells (NSCs) culture in vitro and to establish a simple and efficient method. Methods: single cell suspension was obtained by mechanical blow and trypsin digestion from hippocampal tissue of neonatal SD rats and 1819 SD fetal rats. The cultured cells were identified and induced to differentiate. Finally, the difference between the primary cell acquisition rate, proliferation rate and apoptosis rate was measured and compared between the two groups. Results: the cultured cells from the hippocampal tissues of two different developmental stages showed Nestin positive and Brd U positive neuron-like structures, and the differentiated cells expressed high 尾 鈪，

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